iDeal ChIP-FFPE Kit

• User-friendly, fast, and low toxicity deparaffinization workflow (unlike xylene-based methods)
• Easy recovery of re-hydrated tissue with DiaFilter columns
• Fast chromatin preparation without enzymatic digestion
• Quality: Mild de-crosslinking preserves dsDNA
• Eluted DNA suitable for qPCR analysis or other downstream applications

Successful chromatin shearing using the Bioruptor Pico and iDeal ChIP-FFPE Kit.

Figure 1. Sections of FFPE Mouse liver (stored for 3 years) were processed accordingly to the iDeal ChIP-FFPE protocol. The recovered sheared DNA was analysed using Fragment Analyzer (High Sensitivity NGS Fragment Analysis Kit (DNF-473). The Fragment Analyzer trace shows the fragment size distribution between 100-400 bp ideal for ChIP experiments.

Excellent Histone ChIP results from FFPE samples

Figure 2. Chromatin immunoprecipitation analysis using H3K27ac antibody. ChIP was performed on Mouse liver FFPE samples accordingly to the iDeal ChIP-FFPE protocol using 1 µg of H3K27ac antibody (Diagenode, Cat. no. C15410174)

Excellent TF ChIP results from FFPE samples

Figure 3. Chromatin immunoprecipitation analysis using CTCF antibody. ChIP was performed on Mouse liver FFPE samples accordingly to the iDeal ChIP-FFPE protocol using 1 µl of CTCF antibody (Diagenode, Cat. no. C15410210).

The Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) is the kit compatible with the iDeal ChIP-FFPE kit, recommended for the optimization of chromatin shearing, a critical step for ChIP.

Highly specific ChIP-grade antibodies, provided with batch-specific validation data.

Check out the list of available Primer pairs designed for high specificity to specific genomic regions.