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Other names: VICKZ2, IMP-2, IMP2
Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse, rat: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C. |
| Storage Buffer | PBS containing 50% glycerol, does not contain a preservative. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP | 15 µg per 107 cells | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
| IP | 5 µg per 2.5x106 cells | Fig 3 |
Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2
Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2
Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2
Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP2 (cat. No. C15410350, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP2 protein was subsequently detected by western blot with the IGF2BP2 antibody as described above.
| RIP RIP Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| IGF2BP2 polyclonal antibody datasheet DATASHEET Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Bin... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: IGF2BP2 Antibody (Diagenode Cat# C15410350 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP2 (cat. No. C15410350, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP2 protein was subsequently detected by western blot with the IGF2BP2 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410350', 'old_catalog_number' => '', 'sf_code' => 'C15410350-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 22, 2018', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'modified' => '2022-01-05 15:45:36', 'created' => '2018-06-28 14:44:14', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '533', 'name' => 'IGF2BP2 polyclonal antibody', 'description' => 'IGF2BP2 (UniProtKB/Swiss-Prot entry Q9Y6M1) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the mRNA of certain genes, IGF2, ACTB and MYC. thereby regulating translation. Binding of IFG2BP2 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP2 plays an important role in metabolism and has been associated with susceptibility to diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:25:10', 'created' => '2018-06-27 15:25:10', 'select_label' => '533 - IGF2BP2 polyclonal antibody (001 - 1 μg/μl - Human, mouse, rat: positive. 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Other names: VICKZ2, IMP-2, IMP2' $meta_title = 'IGF2BP2 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4921', 'product_id' => '2977', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1017', 'name' => 'IGF2BP2 polyclonal antibody datasheet', 'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/igf2bp2-polyclonal-antibody-100-ug.pdf', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 15:56:41', 'created' => '2018-07-11 15:56:41', 'ProductsDocument' => array( 'id' => '2656', 'product_id' => '2977', 'document_id' => '1017' ) )include - 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP2 (cat. No. C15410350, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP2 protein was subsequently detected by western blot with the IGF2BP2 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410350', 'old_catalog_number' => '', 'sf_code' => 'C15410350-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 22, 2018', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'modified' => '2022-01-05 15:45:36', 'created' => '2018-06-28 14:44:14', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '533', 'name' => 'IGF2BP2 polyclonal antibody', 'description' => 'IGF2BP2 (UniProtKB/Swiss-Prot entry Q9Y6M1) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the mRNA of certain genes, IGF2, ACTB and MYC. thereby regulating translation. Binding of IFG2BP2 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP2 plays an important role in metabolism and has been associated with susceptibility to diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:25:10', 'created' => '2018-06-27 15:25:10', 'select_label' => '533 - IGF2BP2 polyclonal antibody (001 - 1 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2' $meta_title = 'IGF2BP2 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4921', 'product_id' => '2977', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1017', 'name' => 'IGF2BP2 polyclonal antibody datasheet', 'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/igf2bp2-polyclonal-antibody-100-ug.pdf', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 15:56:41', 'created' => '2018-07-11 15:56:41', 'ProductsDocument' => array( 'id' => '2656', 'product_id' => '2977', 'document_id' => '1017' ) )include - 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP2 (cat. No. C15410350, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP2 protein was subsequently detected by western blot with the IGF2BP2 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410350', 'old_catalog_number' => '', 'sf_code' => 'C15410350-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 22, 2018', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'modified' => '2022-01-05 15:45:36', 'created' => '2018-06-28 14:44:14', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '533', 'name' => 'IGF2BP2 polyclonal antibody', 'description' => 'IGF2BP2 (UniProtKB/Swiss-Prot entry Q9Y6M1) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the mRNA of certain genes, IGF2, ACTB and MYC. thereby regulating translation. Binding of IFG2BP2 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP2 plays an important role in metabolism and has been associated with susceptibility to diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:25:10', 'created' => '2018-06-27 15:25:10', 'select_label' => '533 - IGF2BP2 polyclonal antibody (001 - 1 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2' $meta_title = 'IGF2BP2 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4921', 'product_id' => '2977', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '1017', 'name' => 'IGF2BP2 polyclonal antibody datasheet', 'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/igf2bp2-polyclonal-antibody-100-ug.pdf', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 15:56:41', 'created' => '2018-07-11 15:56:41', 'ProductsDocument' => array( 'id' => '2656', 'product_id' => '2977', 'document_id' => '1017' ) )include - 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP2 (cat. No. C15410350, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP2 protein was subsequently detected by western blot with the IGF2BP2 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410350', 'old_catalog_number' => '', 'sf_code' => 'C15410350-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 22, 2018', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP2 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2', 'modified' => '2022-01-05 15:45:36', 'created' => '2018-06-28 14:44:14', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '533', 'name' => 'IGF2BP2 polyclonal antibody', 'description' => 'IGF2BP2 (UniProtKB/Swiss-Prot entry Q9Y6M1) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the 5’ UTR of the mRNA of certain genes, IGF2, ACTB and MYC. thereby regulating translation. Binding of IFG2BP2 recruits the target mRNA to cytoplasmic protein-RNA complexes (mRNPs) which allows for mRNA transport and transient storage. Further, it modulates the rate of translation and protects the mRNA from degradation. IGF2BP2 plays an important role in metabolism and has been associated with susceptibility to diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:25:10', 'created' => '2018-06-27 15:25:10', 'select_label' => '533 - IGF2BP2 polyclonal antibody (001 - 1 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: VICKZ2, IMP-2, IMP2' $meta_title = 'IGF2BP2 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2977', 'antibody_id' => '533', 'name' => 'IGF2BP2 Antibody', 'description' => '<p><strong>Other names:</strong> VICKZ2, IMP-2, IMP2</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP2 (cat. No. C15410350) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP2 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410350-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP2</strong><br />Whole cell extracts from K562, 293T, HeLa, NIH3T3 and Rat1 cells (lanes 1, 2, 3, 4 and 5, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP2 (cat. No. C15410350) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1017', 'name' => 'IGF2BP2 polyclonal antibody datasheet', 'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP2 (Insulin Like Growth Factor 2 mRNA Binding Protein 2), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/igf2bp2-polyclonal-antibody-100-ug.pdf', 'slug' => 'igf2bp2-polyclonal-antibody-100-ug-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-07-11 15:56:41', 'created' => '2018-07-11 15:56:41', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - 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APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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