Alternative names: KDM5C, MRXJ, MRXSJ, SMCX, XE169
Polyclonal antibody raised in rabbit against mouse Jarid1c (jumonji, AT rich interactive domain 1C), using 3 KLH-conjugated synthetic peptides containing sequences from the inner part of the protein.
| Lot | A474-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Jarid1c (Cat. No. CS-143-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:7,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1c
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against Jarid1c (Cat. No. CS-143-100, lane 2) and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.
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