ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A1224-004 |
---|---|
Concentration | not determined |
Species reactivity | Human |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:500 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human KDM1B (cat. No. CS- PA028-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:14,250.
Datasheet KDM1B CS-PA028-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: KDM1B Antibody (Diagenode Cat# C15310248 Lot# A1224-004). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human KDM1B (cat. No. CS- PA028-100). The plates were coated with the peptides used for immunization of the rabbit. 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Batch-specific data available on the website. Alternative names: AOF1, LSD2, C6orf193', 'modified' => '2022-01-05 15:50:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '229', 'name' => 'KDM1B polyclonal antibody', 'description' => 'KDM1B (UniProtKB/Swiss-Prot entry Q8NB78) is a histone demethylase that demethylates lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. KDM1B is able to demethylate both mono- and di-methylated lysine 4 of histone H3, but has no effect on tri-methylated lysine 4, mono-, di- or tri-methylated lysine 9, mono-, di- or tri-methylated lysine 27, mono-, di- or tri-methylated lysine 36 of histone H3, or on mono-, di- or tri-methylated lysine 20 of histone H4. KDM1B is also required for de novo DNA methylation of a subset of imprinted genes during oogenesis. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1224-004', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'KDM1B (Lysine (K)-specific demethylase 1B) Polyclonal Antibody validated in ELISA. Batch-specific data available on the website. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human KDM1B (cat. No. CS- PA028-100). The plates were coated with the peptides used for immunization of the rabbit. 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It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1224-004', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '737', 'name' => 'Datasheet KDM1B CS-PA028-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_KDM1B_CS-PA028-100.pdf', 'slug' => 'datasheet-kdm1b-cs-pa028-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. 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