| ELISA Enzyme-linked immunosorbent assay. Read more |
Alternative names: ORCA, CENP-33
Polyclonal antibody raised in rabbit against human LRWD1 (leucine-rich repeats and WD repeat domain containing 1) using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.
| Lot | A1016-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:1,000 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LRWD1 (cat. No. CS-PA012-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:24,900.
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