LSD1 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15410125
(pAb-125-050)

50 µg/75 µl

$360.00
Bulk order

Alternative names: BHC110, AOF2, EC1, KDM1

Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.

Lot	A56-001
Concentration	0.67 µg/µl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100	Fig 1
Western Blotting	1:1,000	Fig 2, Fig 3
Immunoprecipitation	2 μg/IP	Fig 3

Validation Data

Figure 1 Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.

Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1
Western blot was performed using respectively 150 μg (lane 1) or 50 μg (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left.

Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1
293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 μg of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410125_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>1:100</td>
<td>Fig 1</td>
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<td>Fig 2, Fig 3</td>
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410125_fig3.jpg" alt="Immunoprecipitation and Western blot analysis" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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<td>Fig 1</td>
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<td>Fig 2, Fig 3</td>
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<p><span>Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.</span></p>',
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410125_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410125_fig3.jpg" alt="Immunoprecipitation and Western blot analysis" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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<th>References</th>
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<td>1:100</td>
<td>Fig 1</td>
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<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, Fig 3</td>
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<td>Immunoprecipitation</td>
<td>2 &mu;g/IP</td>
<td>Fig 3</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<li>Highly sensitive and specific</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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		'modified' => '2022-01-05 15:52:09',
		'created' => '2015-06-29 14:08:20',
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		'id' => '286',
		'name' => 'LSD1 polyclonal antibody',
		'description' => 'LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex which uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated ‘Lys-4’ of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating ‘Lys-9’ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. As a consequence, LSD1 is involved in different processes such as embryonic development, cell differentiation and proliferation, and stem and cancer cell biology.',
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		'lot' => 'A56-001 ',
		'concentration' => '0.67 &micro;g/&micro;l',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, Fig 3</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>2 &mu;g/IP</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'Epigenetic Antibodies Brochure',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet LSD1 pAb-125-050 DATASHEET Datasheet description	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

LSD1 Antibody SDS GB en	Download
LSD1 Antibody SDS US en	Download
LSD1 Antibody SDS DE de	Download
LSD1 Antibody SDS JP ja	Download
LSD1 Antibody SDS BE nl	Download
LSD1 Antibody SDS BE fr	Download
LSD1 Antibody SDS FR fr	Download
LSD1 Antibody SDS ES es	Download

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