LwaCas13a Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15100149

100 µl

$500.00
Bulk order

Other format

C15100149-20

20 µl

Monoclonal antibody raised in mouse against the Cas13a protein from Leptotrichia wadei. (CRISPR associated protein 13, Jennifer Doudna form) using a recombinant protein containing the N-terminus of the protein.

Lot	001
Concentration	Not determined
Species reactivity	Leptotrichia wadei
Type	Monoclonal
Purity	Concentrated cell culture supernatant.
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	TBS containing 0.02 % Na-azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western blotting	1:500	Fig 1

Validation data

Western blot analysis using the Diagenode monoclonal antibody directed against LwaCas13a
Western blot was performed on 20 µg protein extracts from HEK293 cells (lane 1), HEK293 cells spiked with 1 ng recombinant LwaCas13a protein (lane 2) and HEK293 cells transiently expressing full-length Cas13b (lane 3), using the Diagenode antibody against LwaCas13a (Cat. No. C15100149). The antibody was diluted 1:500 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.
Target Description
CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPRassociated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequencespecific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a socalled class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13a is one of the proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.

Documents
LwaCas13a monoclonal antibody datasheet DATASHEET
Monoclonal antibody raised in mouse against the Cas13a protein from Leptotrichia wadei. (CRISPR a... Download

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<p>Use Diagenode's new C2C2 (Cas13a) polyclonal antibody to determine precision and success metrics in CRISPR experiments.</p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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			'description' => '<p>Monoclonal antibody raised in mouse against the <strong>Cas13a protein from Leptotrichia wadei.</strong> (CRISPR associated protein 13, Jennifer Doudna form) using a recombinant protein containing the N-terminus of the protein.</p>',
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15100149_figure-1_wb.png" alt="LwaCas13a Antibody validated for Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Western blot analysis using the Diagenode monoclonal antibody directed against LwaCas13a</strong><br />Western blot was performed on 20 &micro;g protein extracts from HEK293 cells (lane 1), HEK293 cells spiked with 1 ng recombinant LwaCas13a protein (lane 2) and HEK293 cells transiently expressing full-length Cas13b (lane 3), using the Diagenode antibody against LwaCas13a (Cat. No. C15100149). The antibody was diluted 1:500 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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