1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.
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Lot | A2972-0010 |
---|---|
Concentration | 2.1 µg/µl |
Species reactivity | Human, mouse, other (wide range): positive |
Type | Polyclonal |
Purity | Protein A purified polyclonal antibody |
Host | Rabbit |
Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
Storage Buffer | PBS containing 0.05% azide |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
Dot Blot | 1:400 | Fig 1 |
Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I
To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.
1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.
m1I polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against 1-methylinosine (m1I) conjugated to BSA. | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: m1I Antibody (Diagenode Cat# C15410366 Lot# A2972-0010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Batch-specific data available on the website. Full name: 1-methylinosine polyclonal antibody. 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Sample size available.', 'meta_title' => 'm1I Antibody Dot Blot (C15410366) | Diagenode', 'product' => array( 'Product' => array( 'id' => '3092', 'antibody_id' => '567', 'name' => 'm1I Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">1-methylinosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against <strong>1-methylinosine (m1I)</strong> conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div> <div class="small-6 columns"> <p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. 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Batch-specific data available on the website. Full name: 1-methylinosine polyclonal antibody. Sample size available.' $meta_title = 'm1I Antibody Dot Blot (C15410366) | Diagenode' $product = array( 'Product' => array( 'id' => '3092', 'antibody_id' => '567', 'name' => 'm1I Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">1-methylinosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against <strong>1-methylinosine (m1I)</strong> conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div> <div class="small-6 columns"> <p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. 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Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. 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Batch-specific data available on the website. Full name: 1-methylinosine polyclonal antibody. Sample size available.' $meta_title = 'm1I Antibody Dot Blot (C15410366) | Diagenode' $product = array( 'Product' => array( 'id' => '3092', 'antibody_id' => '567', 'name' => 'm1I Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">1-methylinosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against <strong>1-methylinosine (m1I)</strong> conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div> <div class="small-6 columns"> <p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'm1I Antibody validated in Dot Blot. Batch-specific data available on the website. Full name: 1-methylinosine polyclonal antibody. Sample size available.', 'meta_title' => 'm1I Antibody Dot Blot (C15410366) | Diagenode', 'product' => array( 'Product' => array( 'id' => '3092', 'antibody_id' => '567', 'name' => 'm1I Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">1-methylinosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against <strong>1-methylinosine (m1I)</strong> conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div> <div class="small-6 columns"> <p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. 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Batch-specific data available on the website. Full name: 1-methylinosine polyclonal antibody. Sample size available.' $meta_title = 'm1I Antibody Dot Blot (C15410366) | Diagenode' $product = array( 'Product' => array( 'id' => '3092', 'antibody_id' => '567', 'name' => 'm1I Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">1-methylinosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against <strong>1-methylinosine (m1I)</strong> conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div> <div class="small-6 columns"> <p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15410366', 'old_catalog_number' => '', 'sf_code' => 'C15410366-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm1I-polyclonal-antibody', 'meta_title' => 'm1I Antibody Dot Blot (C15410366) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'm1I Antibody validated in Dot Blot. Batch-specific data available on the website. Full name: 1-methylinosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:01:31', 'created' => '2020-08-19 17:07:39', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '567', 'name' => 'm1I Antibody', 'description' => '1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2972-0010', 'concentration' => '2.1 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Dot Blot</td> <td>1:400</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm1I-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-10 15:32:10', 'created' => '2020-08-19 16:58:05', 'select_label' => '567 - m1I Antibody (A2972-0010 - 2.1 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '287', 'name' => 'C15410366', 'product_id' => '3092', 'modified' => '2020-09-10 15:40:50', 'created' => '2020-09-10 15:40:50' ) ), 'Group' => array( 'Group' => array( 'id' => '287', 'name' => 'C15410366', 'product_id' => '3092', 'modified' => '2020-09-10 15:40:50', 'created' => '2020-09-10 15:40:50' ), 'Master' => array( 'id' => '3092', 'antibody_id' => '567', 'name' => 'm1I Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">1-methylinosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against <strong>1-methylinosine (m1I)</strong> conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div> <div class="small-6 columns"> <p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15410366', 'old_catalog_number' => '', 'sf_code' => 'C15410366-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm1I-polyclonal-antibody', 'meta_title' => 'm1I Antibody Dot Blot (C15410366) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'm1I Antibody validated in Dot Blot. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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