1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.
Full name: 1-methylinosine polyclonal antibody
Polyclonal antibody raised in rabbit against 1-methylinosine (m1I) conjugated to BSA.
| Lot | A2972-0010 |
|---|---|
| Concentration | 2.1 µg/µl |
| Species reactivity | Human, mouse, other (wide range): positive |
| Type | Polyclonal |
| Purity | Protein A purified polyclonal antibody |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Dot Blot | 1:400 | Fig 1 |
Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I
To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.
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