N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.
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Full name: N6N6-dimethyladenosine polyclonal antibody
Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (m66A) conjugated to BSA.
| Lot | A3001-0010 |
|---|---|
| Concentration | 2.4 µg/µl |
| Species reactivity | Human, mouse, other (wide range): positive |
| Type | Polyclonal |
| Purity | Protein A purified polyclonal antibody |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP * | 1 - 2 µg per IP | Fig 1, 2 |
| Dot Blotting | 1:400 | Fig 3 |
Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.
Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A
RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A
RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A
To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.
N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.
| m66A polyclonal antibody - Datasheet DATASHEET Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (m66A) conjugated to BSA. | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: m66A Antibody (Diagenode Cat# C15410365 Lot# A3001-0010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. 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We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.' $meta_title = 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode' $product = array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. 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We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm66a-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-18 16:40:16', 'created' => '2020-08-20 11:08:30', 'select_label' => '568 - m66A antibody (A3001-0010 - 2.4 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ) ), 'Group' => array( 'Group' => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ), 'Master' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'product' => array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '568', 'name' => 'm66A antibody', 'description' => 'N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A3001-0010', 'concentration' => '2.4 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP <sup>*</sup></td> <td>1 - 2 µg per IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.' $meta_title = 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode' $product = array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '568', 'name' => 'm66A antibody', 'description' => 'N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A3001-0010', 'concentration' => '2.4 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP <sup>*</sup></td> <td>1 - 2 µg per IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm66a-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-18 16:40:16', 'created' => '2020-08-20 11:08:30', 'select_label' => '568 - m66A antibody (A3001-0010 - 2.4 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ) ), 'Group' => array( 'Group' => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ), 'Master' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'product' => array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '568', 'name' => 'm66A antibody', 'description' => 'N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A3001-0010', 'concentration' => '2.4 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP <sup>*</sup></td> <td>1 - 2 µg per IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm66a-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-18 16:40:16', 'created' => '2020-08-20 11:08:30', 'select_label' => '568 - m66A antibody (A3001-0010 - 2.4 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/m66a-polyclonal-antibody' ) $language = 'en' $meta_keywords = 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody' $meta_description = 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.' $meta_title = 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode' $product = array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '568', 'name' => 'm66A antibody', 'description' => 'N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A3001-0010', 'concentration' => '2.4 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP <sup>*</sup></td> <td>1 - 2 µg per IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm66a-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-18 16:40:16', 'created' => '2020-08-20 11:08:30', 'select_label' => '568 - m66A antibody (A3001-0010 - 2.4 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ) ), 'Group' => array( 'Group' => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ), 'Master' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'product' => array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '568', 'name' => 'm66A antibody', 'description' => 'N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A3001-0010', 'concentration' => '2.4 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP <sup>*</sup></td> <td>1 - 2 µg per IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm66a-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-18 16:40:16', 'created' => '2020-08-20 11:08:30', 'select_label' => '568 - m66A antibody (A3001-0010 - 2.4 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/m66a-polyclonal-antibody' ) $language = 'en' $meta_keywords = 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody' $meta_description = 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.' $meta_title = 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode' $product = array( 'Product' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '568', 'name' => 'm66A antibody', 'description' => 'N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A3001-0010', 'concentration' => '2.4 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive', 'type' => 'Polyclonal', 'purity' => 'Protein A purified polyclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP <sup>*</sup></td> <td>1 - 2 µg per IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'm66a-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-18 16:40:16', 'created' => '2020-08-20 11:08:30', 'select_label' => '568 - m66A antibody (A3001-0010 - 2.4 µg/µl - Human, mouse, other (wide range): positive - Protein A purified polyclonal antibody - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ) ), 'Group' => array( 'Group' => array( 'id' => '288', 'name' => 'C15410365', 'product_id' => '3093', 'modified' => '2020-10-28 15:02:18', 'created' => '2020-10-28 15:02:18' ), 'Master' => array( 'id' => '3093', 'antibody_id' => '568', 'name' => 'm66A Antibody', 'description' => '<p>Full name: <strong><span lang="EN-GB">N6N6-dimethyladenosine polyclonal antibody</span></strong></p> <p><span lang="EN-GB">Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (<strong>m66A</strong>) conjugated to BSA.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1A-RIP.png" alt="m66A Antibody validated for RIP" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig1B.png" alt="m66A Antibody validated in RIP" /></center></div> <div class="small-8 columns"> <p><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig2-RIP.png" alt="m66A Antibody validated for RIP" /></center></div> <div class="small-6 columns"> <p><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p> </div> </div> <div class="extra-spaced"></div> <div class="row"> <div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410365-Fig3-dot.png" alt="m66A Antibody Dot Blot Validation" /></center></div> <div class="small-8 columns"> <p><strong>Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A</strong><br />To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.</p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/21 µl', 'catalog_number' => 'C15410365', 'old_catalog_number' => '', 'sf_code' => 'C15410365-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 7, 2020', 'slug' => 'm66a-polyclonal-antibody', 'meta_title' => 'm66A Antibody RIP and Dot Blotting (C15410365) | Diagenode', 'meta_keywords' => 'm66A Antibody, N6N6-dimethyladenosine polyclonal antibody', 'meta_description' => 'm66A Antibody validated in RIP and Dot Blotting. Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. Sample size available.', 'modified' => '2020-11-19 19:03:54', 'created' => '2020-08-20 11:33:43' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. 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Batch-specific data available on the website. Full name: N6N6-dimethyladenosine polyclonal antibody. 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