MBD3 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15310079
(CS-079-100)

100 µl

$260.00
Bulk order

Polyclonal antibody raised in rabbit against human MBD3 (Methyl-CpG-binding domain protein 3), using three different KLH-conjugated synthetic peptides.

Lot	A127-004
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100 - 1:500	Fig 1
Western Blotting	1:500	Fig 2

Validation Data

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD3 (cat# CS-079-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.

Figure 2. Western blot analysis using the Diagenode antibody directed against MBD3
Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 μg) with the Diagenode antibody against human MBD3 (Cat. No. CS-079-100), diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (diluted 1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). The location of tagged MBD3 is indicated with an arrow.

Documents
Datasheet MBD3 CS-079-100 DATASHEET
Datasheet description Download

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			'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-JP-ja-GHS_1_0.pdf',
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			'name' => 'MBD3 Antibody SDS BE nl',
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			'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-BE-nl-GHS_1_0.pdf',
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			'modified' => '2022-10-03 12:04:21',
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			'name' => 'MBD3 Antibody SDS BE fr',
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			'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-BE-fr-GHS_1_0.pdf',
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			'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-ES-es-GHS_1_0.pdf',
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			'created' => '2022-10-03 12:04:21',
			'ProductsSafetySheet' => array(
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$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
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	'created' => '2015-01-07 09:20:00',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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	'modified' => '2022-10-03 12:04:21',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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	'meta_keywords' => '',
	'meta_description' => 'MBD3  (Methyl-CpG-binding domain protein 3) Polyclonal Antibody  validated  in WB and ELISA.  Batch-specific data available on the website. ',
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig1.jpg" alt="ELISA" width="303" height="286" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
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</div>
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<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig2.jpg" alt="Western blot" width="305" height="168" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD3<br /></strong> <strong>Figure 2A:</strong> Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 &mu;g) with the Diagenode antibody against human MBD3 (Cat. No. CS-079-100), diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. <br /><strong>Figure 2B:</strong> TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (diluted 1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). The location of tagged MBD3 is indicated with an arrow.</small></p>
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<th>References</th>
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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD3 (cat# CS-079-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.</small></p>
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<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig2.jpg" alt="Western blot" width="305" height="168" /></p>
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<th>References</th>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig1.jpg" alt="ELISA" width="303" height="286" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD3 (cat# CS-079-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.</small></p>
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</div>
<div class="row">
<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig2.jpg" alt="Western blot" width="305" height="168" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD3<br /></strong> <strong>Figure 2A:</strong> Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 &mu;g) with the Diagenode antibody against human MBD3 (Cat. No. CS-079-100), diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. <br /><strong>Figure 2B:</strong> TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (diluted 1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). The location of tagged MBD3 is indicated with an arrow.</small></p>
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			'meta_description' => 'MBD3  (Methyl-CpG-binding domain protein 3) Polyclonal Antibody  validated  in WB and ELISA.  Batch-specific data available on the website. ',
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			'description' => 'MBD3 (UniProtKB/Swiss-Prot entry O95983) is a transcriptional repressor which plays a role in gene silencing. Surprisingly, MBD3 does not seem to bind methylated DNA by itself, but acts by the recruitment of histone deacetylases and DNA methyltransferases. MBD3 also forms a heterodimer with MBD2 and is part of the NuRD and the MeCP1 complex. Further, MBD3 interacts with HDAC1, MTA2, DNMT1, p66-alpha and p66-beta.',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>ELISA</td>
<td>1:100 - 1:500</td>
<td>Fig 1</td>
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<td>Fig 2</td>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig1.jpg" alt="ELISA" width="303" height="286" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD3 (cat# CS-079-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310079-MBD3-fig2.jpg" alt="Western blot" width="305" height="168" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD3<br /></strong> <strong>Figure 2A:</strong> Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 &mu;g) with the Diagenode antibody against human MBD3 (Cat. No. CS-079-100), diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. <br /><strong>Figure 2B:</strong> TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (diluted 1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). The location of tagged MBD3 is indicated with an arrow.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD3 (cat# CS-079-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD3<br /></strong> <strong>Figure 2A:</strong> Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 &mu;g) with the Diagenode antibody against human MBD3 (Cat. No. CS-079-100), diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. <br /><strong>Figure 2B:</strong> TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (diluted 1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). The location of tagged MBD3 is indicated with an arrow.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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			'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2022-10-03 12:04:21',
			'created' => '2022-10-03 12:04:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2660',
			'name' => 'MBD3 Antibody SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2022-10-03 12:04:21',
			'created' => '2022-10-03 12:04:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(CS-079-100)</span></small>'
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '3111',
		'product_id' => '2077',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '595',
	'name' => 'Datasheet MBD3 CS-079-100',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_MBD3_CS-079-100.pdf',
	'slug' => 'datasheet-mbd3-cs-079-100',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '696',
		'product_id' => '2077',
		'document_id' => '595'
	)
)
$sds = array(
	'id' => '2660',
	'name' => 'MBD3 Antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/MBD3/SDS-C15310079-MBD3_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2022-10-03 12:04:21',
	'created' => '2022-10-03 12:04:21',
	'ProductsSafetySheet' => array(
		'id' => '4468',
		'product_id' => '2077',
		'safety_sheet_id' => '2660'
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)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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