Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use
Storage
Store at room temperature
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures
The Megaruptor 3 shearing kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The shearing kit has been especially designed for being used with the Megaruptor® 3 (B06010003) and is capable of producing reproducible DNA fragments in the range between 5 kb and higher than 100 kb.
Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use
Storage
Store at room temperature
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures
| Megaruptor® 3 FLYER Your partner in long-read sequencing Designed to provide simple and automated DNA sample prepara... | Download |
| Megaruptor® 3 Quick guide QUICK GUIDE The Megaruptor® 3 is intended for laboratory use. It is not suitable for clinical use. Use of... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Megaruptor 3 Shearing Kit (Diagenode Cat# E07010003). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '3009', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 3', 'description' => '<p><a href="https://go.diagenode.com/l/928883/2023-04-06/3jhlc"><img src="https://www.diagenode.com/img/banners/pacbio-mega-promo-2023.png" /></a></p> <p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p> <p></p> <div style="padding: 30px; background-color: #dad6e3;"> <p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p> <p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p> <p><strong>The NEW Megaruptor 3 HT will give you:</strong></p> <ul> <li><strong>High throughput:</strong> Processes 96 samples in 2 h.</li> <li><strong>Flexibility:</strong> Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.</li> <li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li> <li><strong>Reliable:</strong> enables processing of highly viscous samples.</li> <li><strong>Ergonomic:</strong> Includes a user-friendly interface and has a small footprint.</li> </ul> </div> <p></p> <center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center> <p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>', 'label1' => 'Demonstrated shearing of DNA from 5 -100 kb', 'info1' => '<h3>Excellent reproducibility and size distribution achieved with the Megaruptor 3</h3> <center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center> <p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>', 'label2' => 'Specifications for the Megaruptor', 'info2' => '<table style="width: 941px;"> <tbody> <tr style="height: 55px;"> <th class="text-center" style="width: 296px; height: 55px;"></th> <th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th> <th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Run duration</td> <td class="text-center" style="width: 255px; height: 38px;">20 min – few hours</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 5 min</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Size</td> <td class="text-center" style="width: 255px; height: 38px;">5 – 100 kb</td> <td class="text-center" style="width: 381px; height: 38px;">7 – 40 kb</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Concentration</td> <td class="text-center" style="width: 255px; height: 38px;">0 – 150 ng/μl</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Volume</td> <td class="text-center" style="width: 255px; height: 38px;">40 – 750 μl</td> <td class="text-center" style="width: 381px; height: 38px;">50 – 500 μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Speed settings</td> <td class="text-center" style="width: 255px; height: 38px;">1 – 65</td> <td class="text-center" style="width: 381px; height: 38px;">30 – 59</td> </tr> <tr style="height: 56px;"> <td style="width: 296px; height: 56px;">Throughput</td> <td class="text-center" style="width: 255px; height: 56px;">96 samples in 1 day</td> <td class="text-center" style="width: 381px; height: 56px;">96 samples in < 2h</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Shearing disposables</td> <td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td> </tr> <tr style="height: 32px;"> <td style="width: 296px; height: 32px;">Hardware</td> <td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td> </tr> </tbody> </table>', 'label3' => 'Video', 'info3' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>', 'format' => '1 unit', 'catalog_number' => 'B06010003', 'old_catalog_number' => '', 'sf_code' => 'B06010003-', 'type' => 'DIS', 'search_order' => '', 'price_EUR' => '27500', 'price_USD' => '31500', 'price_GBP' => '24500', 'price_JPY' => '4307875', 'price_CNY' => '0', 'price_AUD' => '78750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3', 'meta_title' => 'The Megaruptor 3', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-04 15:50:30', 'created' => '2018-12-20 16:38:16', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07020001', 'old_catalog_number' => '', 'sf_code' => 'E07020001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '315', 'price_JPY' => '57960', 'price_CNY' => '/', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kit', 'meta_title' => 'Megaruptor DNAFluid+ kit', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor DNAFluid+ kit', 'modified' => '2022-06-09 14:32:19', 'created' => '2020-11-09 15:56:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1042', 'name' => 'Megaruptor® 3 ', 'description' => '<p class="p1"><strong>Your partner in long-read sequencing</strong></p> <p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>', 'image_id' => '186', 'type' => 'flyer', 'url' => 'files/flyers/megaruptor3_flyer.pdf', 'slug' => 'megaruptor3-flyer', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-05 14:23:36', 'created' => '2019-02-19 15:45:41', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1048', 'name' => 'Megaruptor<sup>®</sup> 3 Quick guide', 'description' => '<p>The Megaruptor<sup>®</sup> 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1794', 'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg', 'alt' => 'megaruptor 3 shearing kit', 'modified' => '2019-03-07 13:38:16', 'created' => '2019-03-07 13:38:16', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/megaruptor-3-DNAFluid-kits-bundle' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. 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Not for use in diagnostic or therapeutic procedures</p> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07010003', 'old_catalog_number' => '', 'sf_code' => 'E07010003-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '300', 'price_JPY' => '57960', 'price_CNY' => '', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-shearing-kit', 'meta_title' => 'Megaruptor Shearing kit', 'meta_keywords' => '', 'meta_description' => 'Megaruptor Shearing kit', 'modified' => '2023-03-31 13:53:54', 'created' => '2019-03-07 12:37:06', 'ProductsGroup' => array( 'id' => '407', 'product_id' => '3018', 'group_id' => '355' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/megaruptor-3"><img src="/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010003</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3009" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 3</strong>. 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<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3009"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option 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<option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option 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The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. 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It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( 'id' => '2751', 'product_id' => '3018', 'document_id' => '1048' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '3009', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 3', 'description' => '<p><a href="https://go.diagenode.com/l/928883/2023-04-06/3jhlc"><img src="https://www.diagenode.com/img/banners/pacbio-mega-promo-2023.png" /></a></p> <p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p> <p></p> <div style="padding: 30px; background-color: #dad6e3;"> <p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p> <p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p> <p><strong>The NEW Megaruptor 3 HT will give you:</strong></p> <ul> <li><strong>High throughput:</strong> Processes 96 samples in 2 h.</li> <li><strong>Flexibility:</strong> Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.</li> <li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li> <li><strong>Reliable:</strong> enables processing of highly viscous samples.</li> <li><strong>Ergonomic:</strong> Includes a user-friendly interface and has a small footprint.</li> </ul> </div> <p></p> <center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center> <p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>', 'label1' => 'Demonstrated shearing of DNA from 5 -100 kb', 'info1' => '<h3>Excellent reproducibility and size distribution achieved with the Megaruptor 3</h3> <center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center> <p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>', 'label2' => 'Specifications for the Megaruptor', 'info2' => '<table style="width: 941px;"> <tbody> <tr style="height: 55px;"> <th class="text-center" style="width: 296px; height: 55px;"></th> <th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th> <th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Run duration</td> <td class="text-center" style="width: 255px; height: 38px;">20 min – few hours</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 5 min</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Size</td> <td class="text-center" style="width: 255px; height: 38px;">5 – 100 kb</td> <td class="text-center" style="width: 381px; height: 38px;">7 – 40 kb</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Concentration</td> <td class="text-center" style="width: 255px; height: 38px;">0 – 150 ng/μl</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Volume</td> <td class="text-center" style="width: 255px; height: 38px;">40 – 750 μl</td> <td class="text-center" style="width: 381px; height: 38px;">50 – 500 μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Speed settings</td> <td class="text-center" style="width: 255px; height: 38px;">1 – 65</td> <td class="text-center" style="width: 381px; height: 38px;">30 – 59</td> </tr> <tr style="height: 56px;"> <td style="width: 296px; height: 56px;">Throughput</td> <td class="text-center" style="width: 255px; height: 56px;">96 samples in 1 day</td> <td class="text-center" style="width: 381px; height: 56px;">96 samples in < 2h</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Shearing disposables</td> <td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td> </tr> <tr style="height: 32px;"> <td style="width: 296px; height: 32px;">Hardware</td> <td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td> </tr> </tbody> </table>', 'label3' => 'Video', 'info3' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>', 'format' => '1 unit', 'catalog_number' => 'B06010003', 'old_catalog_number' => '', 'sf_code' => 'B06010003-', 'type' => 'DIS', 'search_order' => '', 'price_EUR' => '27500', 'price_USD' => '31500', 'price_GBP' => '24500', 'price_JPY' => '4307875', 'price_CNY' => '0', 'price_AUD' => '78750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3', 'meta_title' => 'The Megaruptor 3', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-04 15:50:30', 'created' => '2018-12-20 16:38:16', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07020001', 'old_catalog_number' => '', 'sf_code' => 'E07020001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '315', 'price_JPY' => '57960', 'price_CNY' => '/', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kit', 'meta_title' => 'Megaruptor DNAFluid+ kit', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor DNAFluid+ kit', 'modified' => '2022-06-09 14:32:19', 'created' => '2020-11-09 15:56:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1042', 'name' => 'Megaruptor® 3 ', 'description' => '<p class="p1"><strong>Your partner in long-read sequencing</strong></p> <p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>', 'image_id' => '186', 'type' => 'flyer', 'url' => 'files/flyers/megaruptor3_flyer.pdf', 'slug' => 'megaruptor3-flyer', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-05 14:23:36', 'created' => '2019-02-19 15:45:41', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1048', 'name' => 'Megaruptor<sup>®</sup> 3 Quick guide', 'description' => '<p>The Megaruptor<sup>®</sup> 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1794', 'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg', 'alt' => 'megaruptor 3 shearing kit', 'modified' => '2019-03-07 13:38:16', 'created' => '2019-03-07 13:38:16', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/megaruptor-3-DNAFluid-kits-bundle' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. 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columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3137" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3137" id="CartAdd/3137Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3137" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Megaruptor 3 DNAFluid+ Kit</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Megaruptor 3 DNAFluid+ Kit', 'E07020001', '390', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Megaruptor 3 DNAFluid+ Kit', 'E07020001', '390', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="megaruptor-3-DNAFluid-kit" data-reveal-id="cartModal-3137" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor 3 DNAFluid+ Kit</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/megaruptor-3-DNAFluid-kits-bundle"><img src="/img/product/shearing_technologies/E0701003-mega3-shearing-kit.jpg" alt="megaruptor 3 shearing kit" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07030001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3219" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3219" id="CartAdd/3219Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" 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class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="megaruptor-3-DNAFluid-kits-bundle" data-reveal-id="cartModal-3219" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor Shearing Kit/DNAFluid+ Bundle</h6> </div> </div> </li> ' $related = array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41', 'ProductsRelated' => array( 'id' => '4826', 'product_id' => '3018', 'related_id' => '3219' ), 'Image' => array( (int) 0 => array( 'id' => '1794', 'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg', 'alt' => 'megaruptor 3 shearing kit', 'modified' => '2019-03-07 13:38:16', 'created' => '2019-03-07 13:38:16', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07020001', 'old_catalog_number' => '', 'sf_code' => 'E07020001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '315', 'price_JPY' => '57960', 'price_CNY' => '/', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kit', 'meta_title' => 'Megaruptor DNAFluid+ kit', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor DNAFluid+ kit', 'modified' => '2022-06-09 14:32:19', 'created' => '2020-11-09 15:56:07', 'ProductsGroup' => array( 'id' => '406', 'product_id' => '3137', 'group_id' => '355' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1048', 'name' => 'Megaruptor<sup>®</sup> 3 Quick guide', 'description' => '<p>The Megaruptor<sup>®</sup> 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( 'id' => '2751', 'product_id' => '3018', 'document_id' => '1048' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '3009', 'antibody_id' => null, 'name' => 'Megaruptor<sup>®</sup> 3', 'description' => '<p><a href="https://go.diagenode.com/l/928883/2023-04-06/3jhlc"><img src="https://www.diagenode.com/img/banners/pacbio-mega-promo-2023.png" /></a></p> <p>The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from <strong>5 kb - 100 kb</strong>. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p> <p></p> <div style="padding: 30px; background-color: #dad6e3;"> <p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p> <p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p> <p><strong>The NEW Megaruptor 3 HT will give you:</strong></p> <ul> <li><strong>High throughput:</strong> Processes 96 samples in 2 h.</li> <li><strong>Flexibility:</strong> Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.</li> <li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li> <li><strong>Reliable:</strong> enables processing of highly viscous samples.</li> <li><strong>Ergonomic:</strong> Includes a user-friendly interface and has a small footprint.</li> </ul> </div> <p></p> <center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center> <p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>', 'label1' => 'Demonstrated shearing of DNA from 5 -100 kb', 'info1' => '<h3>Excellent reproducibility and size distribution achieved with the Megaruptor 3</h3> <center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center> <p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>', 'label2' => 'Specifications for the Megaruptor', 'info2' => '<table style="width: 941px;"> <tbody> <tr style="height: 55px;"> <th class="text-center" style="width: 296px; height: 55px;"></th> <th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th> <th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Run duration</td> <td class="text-center" style="width: 255px; height: 38px;">20 min – few hours</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 5 min</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Size</td> <td class="text-center" style="width: 255px; height: 38px;">5 – 100 kb</td> <td class="text-center" style="width: 381px; height: 38px;">7 – 40 kb</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Concentration</td> <td class="text-center" style="width: 255px; height: 38px;">0 – 150 ng/μl</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Volume</td> <td class="text-center" style="width: 255px; height: 38px;">40 – 750 μl</td> <td class="text-center" style="width: 381px; height: 38px;">50 – 500 μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Speed settings</td> <td class="text-center" style="width: 255px; height: 38px;">1 – 65</td> <td class="text-center" style="width: 381px; height: 38px;">30 – 59</td> </tr> <tr style="height: 56px;"> <td style="width: 296px; height: 56px;">Throughput</td> <td class="text-center" style="width: 255px; height: 56px;">96 samples in 1 day</td> <td class="text-center" style="width: 381px; height: 56px;">96 samples in < 2h</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Shearing disposables</td> <td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td> </tr> <tr style="height: 32px;"> <td style="width: 296px; height: 32px;">Hardware</td> <td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td> </tr> </tbody> </table>', 'label3' => 'Video', 'info3' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>', 'format' => '1 unit', 'catalog_number' => 'B06010003', 'old_catalog_number' => '', 'sf_code' => 'B06010003-', 'type' => 'DIS', 'search_order' => '', 'price_EUR' => '27500', 'price_USD' => '31500', 'price_GBP' => '24500', 'price_JPY' => '4307875', 'price_CNY' => '0', 'price_AUD' => '78750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3', 'meta_title' => 'The Megaruptor 3', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-04 15:50:30', 'created' => '2018-12-20 16:38:16', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07020001', 'old_catalog_number' => '', 'sf_code' => 'E07020001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '315', 'price_JPY' => '57960', 'price_CNY' => '/', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kit', 'meta_title' => 'Megaruptor DNAFluid+ kit', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor DNAFluid+ kit', 'modified' => '2022-06-09 14:32:19', 'created' => '2020-11-09 15:56:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1042', 'name' => 'Megaruptor® 3 ', 'description' => '<p class="p1"><strong>Your partner in long-read sequencing</strong></p> <p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>', 'image_id' => '186', 'type' => 'flyer', 'url' => 'files/flyers/megaruptor3_flyer.pdf', 'slug' => 'megaruptor3-flyer', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-05 14:23:36', 'created' => '2019-02-19 15:45:41', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1048', 'name' => 'Megaruptor<sup>®</sup> 3 Quick guide', 'description' => '<p>The Megaruptor<sup>®</sup> 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1794', 'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg', 'alt' => 'megaruptor 3 shearing kit', 'modified' => '2019-03-07 13:38:16', 'created' => '2019-03-07 13:38:16', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/megaruptor-3-DNAFluid-kits-bundle' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. 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<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/megaruptor-3-DNAFluid-kits-bundle"><img src="/img/product/shearing_technologies/E0701003-mega3-shearing-kit.jpg" alt="megaruptor 3 shearing kit" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07030001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3219" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3219" id="CartAdd/3219Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" 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class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="megaruptor-3-DNAFluid-kits-bundle" data-reveal-id="cartModal-3219" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor Shearing Kit/DNAFluid+ Bundle</h6> </div> </div> </li> ' $related = array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. 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The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. 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Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for <strong>8 samples to be processed simultaneously </strong>without additional user input. A <a href="https://www.diagenode.com/p/megaruptor-cassette-12">12 sample cassette</a> <span style="font-weight: 400;">is also available for purchase separately for increased throughput. </span>Just set the desired parameters and the automated system takes care of the rest. <span style="font-weight: 400;">The shearing with the Megaruptor leads to optimal long-read sequencing using <strong>PacBio®'s</strong>, and <strong>Oxford Nanopore™</strong> technologies' systems.</span></p> <p></p> <div style="padding: 30px; background-color: #dad6e3;"> <p style="font-size: 1.2em; color: #2b2967;"><strong>Discover the NEW Megaruptor 3 HT</strong></p> <p>The <strong>Megaruptor 3 HT</strong> offers an <strong>ultrafast protocol</strong> for increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the <strong>Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb</strong>.</p> <p><strong>The NEW Megaruptor 3 HT will give you:</strong></p> <ul> <li><strong>High throughput:</strong> Processes 96 samples in 2 h.</li> <li><strong>Flexibility:</strong> Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.</li> <li><strong>Unmatched quality:</strong> Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.</li> <li><strong>Reliable:</strong> enables processing of highly viscous samples.</li> <li><strong>Ergonomic:</strong> Includes a user-friendly interface and has a small footprint.</li> </ul> </div> <p></p> <center><a href="https://go.diagenode.com/dnafluid" class="tiny details button">Validation of the DNA Fluid+ Kit on viscous samples with the Circulomics Nanobind CBB Big DNA Kit.</a></center><center></center><center><iframe width="700" height="394" src="https://www.youtube.com/embed/VaWEK1vjbOY?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center> <p><a href="https://www.diagenode.com/en/documents/pacbio-megaruptor-application-note"><img src="https://www.diagenode.com/img/banners/pacbio-mega-banner.png" /></a></p>', 'label1' => 'Demonstrated shearing of DNA from 5 -100 kb', 'info1' => '<h3>Excellent reproducibility and size distribution achieved with the Megaruptor 3</h3> <center><img src="https://www.diagenode.com/img/product/shearing_technologies/mega3-results.png" /></center> <p><strong>A</strong>: Fragment Analyzer profiles of human genomic DNA samples (25 ng/μl; 200 μl/sample) sheared to 6, 10 and 30 kb. <strong>B</strong>: DNA samples sheared at different speed settings of the Megaruptor were analyzed by Pulsed Field Gel Electrophoresis (PFGE) in 1% agarose gel. (NS: Not Sheared)</p>', 'label2' => 'Specifications for the Megaruptor', 'info2' => '<table style="width: 941px;"> <tbody> <tr style="height: 55px;"> <th class="text-center" style="width: 296px; height: 55px;"></th> <th class="text-center" style="width: 255px; height: 55px; background-color: #2b2967; color: white;">Megaruptor 3 regular</th> <th class="text-center" style="width: 381px; height: 55px; background-color: #af3db2; color: white;">Megaruptor 3 HT with ultrafast protocol</th> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Run duration</td> <td class="text-center" style="width: 255px; height: 38px;">20 min – few hours</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 5 min</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Size</td> <td class="text-center" style="width: 255px; height: 38px;">5 – 100 kb</td> <td class="text-center" style="width: 381px; height: 38px;">7 – 40 kb</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Concentration</td> <td class="text-center" style="width: 255px; height: 38px;">0 – 150 ng/μl</td> <td class="text-center" style="width: 381px; height: 38px;">1 – 50 ng/μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Volume</td> <td class="text-center" style="width: 255px; height: 38px;">40 – 750 μl</td> <td class="text-center" style="width: 381px; height: 38px;">50 – 500 μl</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Speed settings</td> <td class="text-center" style="width: 255px; height: 38px;">1 – 65</td> <td class="text-center" style="width: 381px; height: 38px;">30 – 59</td> </tr> <tr style="height: 56px;"> <td style="width: 296px; height: 56px;">Throughput</td> <td class="text-center" style="width: 255px; height: 56px;">96 samples in 1 day</td> <td class="text-center" style="width: 381px; height: 56px;">96 samples in < 2h</td> </tr> <tr style="height: 38px;"> <td style="width: 296px; height: 38px;">Shearing disposables</td> <td class="text-center" style="width: 636px; height: 38px;" colspan="2">Multiple orifice hydrodynamic pore</td> </tr> <tr style="height: 32px;"> <td style="width: 296px; height: 32px;">Hardware</td> <td class="text-center" style="width: 636px; height: 32px;" colspan="2">State-of-the-art electronics<br />Large memory for new features<br />Extensive fine-tuning options<br />Robust titanium pins</td> </tr> </tbody> </table>', 'label3' => 'Video', 'info3' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center>', 'format' => '1 unit', 'catalog_number' => 'B06010003', 'old_catalog_number' => '', 'sf_code' => 'B06010003-', 'type' => 'DIS', 'search_order' => '', 'price_EUR' => '27500', 'price_USD' => '31500', 'price_GBP' => '24500', 'price_JPY' => '4307875', 'price_CNY' => '0', 'price_AUD' => '78750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3', 'meta_title' => 'The Megaruptor 3', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-04 15:50:30', 'created' => '2018-12-20 16:38:16', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07020001', 'old_catalog_number' => '', 'sf_code' => 'E07020001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '315', 'price_JPY' => '57960', 'price_CNY' => '/', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kit', 'meta_title' => 'Megaruptor DNAFluid+ kit', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor DNAFluid+ kit', 'modified' => '2022-06-09 14:32:19', 'created' => '2020-11-09 15:56:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '68', 'position' => '1', 'parent_id' => '8', 'name' => 'Megaruptor accessories', 'description' => '<p><span>The Megaruptor</span><sup><span>® </span></sup><span>uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the <strong>Hydrotube</strong> through an array of uniform pores - the <strong>Hydropore</strong>. The resulting turbulent flow stretches and breaks the DNA strands. The Megaruptor<sup>®</sup> accessories ensures that DNA will reach a minimum and uniform length.</span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'megaruptor-accessories', 'cookies_tag_id' => null, 'meta_keywords' => ' Megaruptor®,DNA sample,shearing Technologies', 'meta_description' => 'Megaruptor® uses the principle of mechanical shearing to fragment DNA. As DNA in solution is pumped from the Hydrotube through an array of uniform pores - the Hydropore. ', 'meta_title' => 'Accessories for Megaruptor® | Diagenode', 'modified' => '2020-07-06 13:00:14', 'created' => '2015-07-23 11:48:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1042', 'name' => 'Megaruptor® 3 ', 'description' => '<p class="p1"><strong>Your partner in long-read sequencing</strong></p> <p class="p1">Designed to provide simple and automated <span class="s1">DNA sample preparation</span>, the Megaruptor gives state-ofthe-art shearing performance which is independent of the source, concentration, temperature, or salt content of a DNA sample, providing researcher with ultimate flexibility and ease of use. The shearing with the Megaruptor leads to optimal long-read sequencing using <span class="s1">PacBio</span><span class="s2">®</span>, and <span class="s1">Oxford Nanopore™ </span>technologies.</p>', 'image_id' => '186', 'type' => 'flyer', 'url' => 'files/flyers/megaruptor3_flyer.pdf', 'slug' => 'megaruptor3-flyer', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-05 14:23:36', 'created' => '2019-02-19 15:45:41', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1048', 'name' => 'Megaruptor<sup>®</sup> 3 Quick guide', 'description' => '<p>The Megaruptor<sup>®</sup> 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1794', 'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg', 'alt' => 'megaruptor 3 shearing kit', 'modified' => '2019-03-07 13:38:16', 'created' => '2019-03-07 13:38:16', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/megaruptor-3-DNAFluid-kits-bundle' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41' ), (int) 1 => array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. 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Not for use in diagnostic or therapeutic procedures</p> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<center><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></center> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07010003', 'old_catalog_number' => '', 'sf_code' => 'E07010003-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '300', 'price_JPY' => '57960', 'price_CNY' => '', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-shearing-kit', 'meta_title' => 'Megaruptor Shearing kit', 'meta_keywords' => '', 'meta_description' => 'Megaruptor Shearing kit', 'modified' => '2023-03-31 13:53:54', 'created' => '2019-03-07 12:37:06', 'ProductsGroup' => array( 'id' => '407', 'product_id' => '3018', 'group_id' => '355' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/megaruptor-3"><img src="/img/product/shearing_technologies/B06010003_megaruptor3.jpg" alt="Megaruptor 3" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B06010003</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3009" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Megaruptor<sup>®</sup> 3</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/en/quotes/quote?id=3009" id="Quote-3009" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3009" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="TWk1dCtpUGJTQi9ZcWxaKzhhaElOQT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="megaruptor-3" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 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<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3009"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha672aaac354b65"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3009" class="alert button expand" form="Quote-3009" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3009 #state-3009").parent().parent().show(); } else { $("#Quote-3009 #state-3009").parent().parent().hide(); $("#Quote-3009 #state-3009").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3009" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="megaruptor-3" data-reveal-id="quoteModal-3009" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor® 3</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/megaruptor-3-DNAFluid-kit"><img src="/img/product/shearing_technologies/E0701003-mega3-shearing-kit.jpg" alt="megaruptor 3 shearing kit" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3137" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3137" id="CartAdd/3137Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3137" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Megaruptor 3 DNAFluid+ Kit</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Megaruptor 3 DNAFluid+ Kit', 'E07020001', '390', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Megaruptor 3 DNAFluid+ Kit', 'E07020001', '390', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="megaruptor-3-DNAFluid-kit" data-reveal-id="cartModal-3137" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor 3 DNAFluid+ Kit</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/megaruptor-3-DNAFluid-kits-bundle"><img src="/img/product/shearing_technologies/E0701003-mega3-shearing-kit.jpg" alt="megaruptor 3 shearing kit" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">E07030001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3219" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/3219" id="CartAdd/3219Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3219" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Megaruptor Shearing Kit/DNAFluid+ Bundle</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Megaruptor Shearing Kit/DNAFluid+ Bundle', 'E07030001', '580', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Megaruptor Shearing Kit/DNAFluid+ Bundle', 'E07030001', '580', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="megaruptor-3-DNAFluid-kits-bundle" data-reveal-id="cartModal-3219" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Megaruptor Shearing Kit/DNAFluid+ Bundle</h6> </div> </div> </li> ' $related = array( 'id' => '3219', 'antibody_id' => null, 'name' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07030001', 'old_catalog_number' => '', 'sf_code' => 'E07030001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '580', 'price_USD' => '580', 'price_GBP' => '455', 'price_JPY' => '90855', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kits-bundle', 'meta_title' => 'Megaruptor Shearing Kit/DNAFluid+ Bundle', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor Shearing Kit - DNAFluid+ Bundle', 'modified' => '2023-03-31 13:55:03', 'created' => '2022-06-09 14:27:41', 'ProductsRelated' => array( 'id' => '4826', 'product_id' => '3018', 'related_id' => '3219' ), 'Image' => array( (int) 0 => array( 'id' => '1794', 'name' => 'product/shearing_technologies/E0701003-mega3-shearing-kit.jpg', 'alt' => 'megaruptor 3 shearing kit', 'modified' => '2019-03-07 13:38:16', 'created' => '2019-03-07 13:38:16', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3137', 'antibody_id' => null, 'name' => 'Megaruptor 3 DNAFluid+ Kit', 'description' => '<h2 style="text-align: left;">The DNAFluid+ Kit for viscous DNA</h2> <p><span style="font-weight: 400;">The DNAFluid+ Kit for viscous DNA eliminates the challenges of using </span><b>highly viscous extracted DNA samples </b><span style="font-weight: 400;">for any downstream steps and QC. The</span><span style="font-weight: 400;"> </span><b><i>DNAFluid+ Kit </i></b><span style="font-weight: 400;">consists of proprietary "<span>Hydropore-Syringe" and "Hydro Tube" </span>shearing accessories that reduce the viscosity of DNA by pre-conditioning high molecular weight DNA prior to shearing on the </span><b><i>Megaruptor 3. </i></b><span style="font-weight: 400;">This combination of the DNAFluid+ Kit and the Megaruptor 3 together achieve effective, automated homogenization of highly viscous DNA samples while keeping the integrity of the DNA and full control of the DNA size prior to any downstream applications. </span></p> <p><br /> <a href="https://www.diagenode.com/files/products/kits/Application_Note-DNAFluid.pdf"><img src="https://www.diagenode.com/img/banners/banner-appnote-dnafluid.png" /></a></p>', 'label1' => 'Data', 'info1' => '<h3>Validation data: DNAFluid+ Kit for viscous DNA</h3> <p><span>Validation of the DNA Fluid+ Kit on viscous samples is shown below. HMW DNA was extracted from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit. First, the sample was pre-sheared using the DNA Fluid+ Kit (speed 59) and then diluted to 50 ng/uL and sheared using the Long Hydropore (speed 31) on the Megaruptor 3 system. PacBio HiFi sequencing was performed on the sheared DNA using a 30 hr movie, SMRTbell Express Template Prep Kit 2.0, Binding Kit 2.0, and Sequel II Sequencing Kit 2.0, and Circulomics SRE XS size selection. These results show that Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition DNA for Long Hydropore shearing without clogging.</span></p> <h2 class="card-title text-lg">DNA Fluid+ Kit effectively pre-conditions HMW DNA to allow consistent shearing across samples of varying viscosity.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid1.png" /></p> <p><span>Fig.1. The Femto Pulse traces show fragment size distributions of: 1) native HMW DNA isolated from GM12878 cells using the Circulomics Nanobind CBB Big DNA Kit (black curve), 2) after pre-shearing with the Diagenode DNA Fluid+ Kit (blue curve) and 3) after shearing to ~18 kb with the Diagenode Long Hydropore using Megaruptor3 (red curve). Pre-shearing the HMW DNA enables more consistent shearing performance across samples of varying viscosity.</span></p> <h2 class="card-title text-lg">Excellent read length distribution of sequencing results using DNA treated with DNA Fluid+ Kit.</h2> <p><img src="https://www.diagenode.com/img/product/shearing_technologies/DNAFluid2.png" width="600" height="449" /></p> <p class="text-xs">Fig. 2. After shearing the gDNA that was pre-conditioned with the Diagenode DNA Fluid+ Kit, HiFi read length distribution of GM12878 SMRTbell library shows excellent read length distribution. Sequencing was performed on the PacBio Sequel II system.</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Characteristics', 'info2' => '<p>The Megaruptor 3 DNAFluid+ kit includes ready-to-use assemblies of Hydropore-Syringe and Hydro Tubes for 16 samples. The kit has been especially designed for being used with the Megaruptor<sup>®</sup> 3 (B06010003). Please note, the DNAFluid+ Kit pre-conditions viscous DNA. Shearing is accomplished with the use of the Megaruptor 3 in conjunction with this kit.</p> <p>Sterile Hydropores, Syringes, and Hydro Tubes are intended for single use</p> <p><strong>Storage</strong><br /> Store at room temperature</p> <p><strong>Precautions</strong><br /> This product is for research use only. Not for use in diagnostic or therapeutic procedures</p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'Video', 'info3' => '<p style="text-align: center;"><iframe width="700" height="394" src="https://www.youtube.com/embed/zVhqnkQL8zo?rel=0" frameborder="0" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="allowfullscreen"></iframe></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '16 samples', 'catalog_number' => 'E07020001', 'old_catalog_number' => '', 'sf_code' => 'E07020001-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '370', 'price_USD' => '390', 'price_GBP' => '315', 'price_JPY' => '57960', 'price_CNY' => '/', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'China', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'megaruptor-3-DNAFluid-kit', 'meta_title' => 'Megaruptor DNAFluid+ kit', 'meta_keywords' => 'homogenization, viscous, HMW, DNA', 'meta_description' => 'Megaruptor DNAFluid+ kit', 'modified' => '2022-06-09 14:32:19', 'created' => '2020-11-09 15:56:07', 'ProductsGroup' => array( 'id' => '406', 'product_id' => '3137', 'group_id' => '355' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1048', 'name' => 'Megaruptor<sup>®</sup> 3 Quick guide', 'description' => '<p>The Megaruptor<sup>®</sup> 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.</p>', 'image_id' => '185', 'type' => 'Quick guide', 'url' => 'files/products/shearing_technology/megaruptor/megaruptor-quick-guide.pdf', 'slug' => 'megaruptor-3-quick-guide', 'meta_keywords' => 'Megaruptor 3 Quick guide', 'meta_description' => 'Megaruptor 3 Quick guide', 'modified' => '2019-03-21 16:04:50', 'created' => '2019-03-21 16:04:50', 'ProductsDocument' => array( 'id' => '2751', 'product_id' => '3018', 'document_id' => '1048' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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