Diagenode

Megaruptor® 2

 DNA Shearing, RNA shearing and Chromatin shearing
Tight fragment size distribution
Hydropores eliminate clogging
High quality libraires
Tuneable to between 3 - 75 kb
1 to 2 samples in series
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Catalog Number
Format
B06010002
1 unit

The Megaruptor® 2 was designed to provide the best experience with the fragmentation of DNA from 3 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly interface allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design. The shearing with the Megaruptor leads to optimal long-read sequencing using PacBio®'s, and Oxford Nanopore™ technologies' systems.

TESTIMONIAL

Established in March 15th 2000, DNA Link, Inc. has developed as one of the leading full genomic service provider with its extensive 17 years of experience. DNA Link has been involved in numerous genomic researches including many national projects, and contributed to the expansion of the genomic industry.

On one hand, DNA Link was the very first company to introduce the RS II system from Pacific Bioscience in Asia, and has been approved as one of the eight global certified service providers for PacBio. Since 2012, DNA Link has been providing quality results that are controlled under strict criteria and successfully assisted numerous research projects.

PacBio platforms’ strongest upside is that it generates the longest reads among all sequencing platforms, and constructing long-read libraries using high molecular weight DNA is the most crucial part of utilizing the platforms. Within the PacBio library preparation process, shearing the DNA molecules accordingly to the library size is the initial step for a successful library construction. Sufficient library yield can be obtained only if the DNA molecules are sheared within the targeted size.

DNA Link has been using the Megaruptor instrument from Diagenode for this shearing process, as it is easy to use and has less contamination risk for using disposable hydropores, which contributes to the overall efficiency of the laboratory logistics.

Read our new Nature Methods publication about the Megaruptor 2 and best NGS library preparation practices

Hyeyoon, Jang - Lab Manager - Seoul, Korea

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