| Datasheet MethylTaq DNA polymerase DATASHEET Datasheet description | Download |
MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymerase that completely lacks any activity below 74°C thus avoiding non-specific priming at low temperature. This highly robust enzyme produces excellent results in demanding applications and it is recommended for PCR after hMeDIP (Cat. No. C02010031) or after Bisulfite Conversion (Cat. No. C02030030).
| Datasheet MethylTaq DNA polymerase DATASHEET Datasheet description | Download |
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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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It enables <span>to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by <strong>PCR amplification</strong> or <strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <h2>How it works</h2> <p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged. <span>The DNA is then amplified by PCR where the uracils are converted to thymines. </span></p> <p style="text-align: center;"><span></span></p> <p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> <h2>Advantages</h2> <ul class="nobullet" style="font-size: 19px;"> <li><i class="fa fa-arrow-circle-right"></i><strong> </strong><strong>Single nucleotide</strong> resolution</li> <li><i class="fa fa-arrow-circle-right"></i><strong> Gene-specific </strong>and <strong>genome-wide</strong><span> analyses</span></li> <li><i class="fa fa-arrow-circle-right"></i><strong> NGS</strong><span> </span>compatible</li> </ul> <h2>Downstream analysis techniques</h2> <ul class="square"> <li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit V2</a></li> <li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li> </ul> <p></p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bisulfite-conversion', 'cookies_tag_id' => null, 'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing', 'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '56', 'position' => '6', 'parent_id' => '12', 'name' => 'PCR enzymes and nucleotides', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'pcr-enzymes-and-nucleotides', 'cookies_tag_id' => null, 'meta_keywords' => 'PCR enzymes and nucleotides, methylated DNA ,DNA methylation', 'meta_description' => 'Diagenode's PCR enzymes and nucleotides Optimized for amplification of methylated DNA ', 'meta_title' => 'PCR enzymes and nucleotides for DNA methylation | Diagenode', 'modified' => '2019-07-03 10:42:59', 'created' => '2015-07-08 09:57:30', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '87', 'position' => '1', 'parent_id' => '86', 'name' => 'PCR enzymes and Mixes', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'PCR-enzymes-and-mixes', 'cookies_tag_id' => null, 'meta_keywords' => 'PCR enzymes and RT-PCR Mastermix ', 'meta_description' => 'Diagenode offers DNA polymerase and RT-PCR Mastermix ', 'meta_title' => 'PCR enzymes and RT-PCR Mastermix | Diagenode ', 'modified' => '2016-01-21 15:01:47', 'created' => '2015-09-16 23:10:49', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '54', 'position' => '1', 'parent_id' => '12', 'name' => 'Bisulfite conversion', 'description' => '<div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p>Sodium bisulfite conversion of genomic DNA is the most commonly used method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by <strong>PCR amplification</strong> or <strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <h2>How it works</h2> <p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged. <span>The DNA is then amplified by PCR where the uracils are converted to thymines. </span></p> <p style="text-align: center;"><span></span></p> <p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> <h2>Advantages</h2> <ul class="nobullet" style="font-size: 19px;"> <li><i class="fa fa-arrow-circle-right"></i><strong> </strong><strong>Single nucleotide</strong> resolution</li> <li><i class="fa fa-arrow-circle-right"></i><strong> Gene-specific </strong>and <strong>genome-wide</strong><span> analyses</span></li> <li><i class="fa fa-arrow-circle-right"></i><strong> NGS</strong><span> </span>compatible</li> </ul> <h2>Downstream analysis techniques</h2> <ul class="square"> <li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit V2</a></li> <li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li> </ul> <p></p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bisulfite-conversion', 'cookies_tag_id' => null, 'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing', 'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '56', 'position' => '6', 'parent_id' => '12', 'name' => 'PCR enzymes and nucleotides', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'pcr-enzymes-and-nucleotides', 'cookies_tag_id' => null, 'meta_keywords' => 'PCR enzymes and nucleotides, methylated DNA ,DNA methylation', 'meta_description' => 'Diagenode's PCR enzymes and nucleotides Optimized for amplification of methylated DNA ', 'meta_title' => 'PCR enzymes and nucleotides for DNA methylation | Diagenode', 'modified' => '2019-07-03 10:42:59', 'created' => '2015-07-08 09:57:30', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '87', 'position' => '1', 'parent_id' => '86', 'name' => 'PCR enzymes and Mixes', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'PCR-enzymes-and-mixes', 'cookies_tag_id' => null, 'meta_keywords' => 'PCR enzymes and RT-PCR Mastermix ', 'meta_description' => 'Diagenode offers DNA polymerase and RT-PCR Mastermix ', 'meta_title' => 'PCR enzymes and RT-PCR Mastermix | Diagenode ', 'modified' => '2016-01-21 15:01:47', 'created' => '2015-09-16 23:10:49', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '54', 'position' => '1', 'parent_id' => '12', 'name' => 'Bisulfite conversion', 'description' => '<div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p>Sodium bisulfite conversion of genomic DNA is the most commonly used method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by <strong>PCR amplification</strong> or <strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <h2>How it works</h2> <p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged. <span>The DNA is then amplified by PCR where the uracils are converted to thymines. </span></p> <p style="text-align: center;"><span></span></p> <p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> <h2>Advantages</h2> <ul class="nobullet" style="font-size: 19px;"> <li><i class="fa fa-arrow-circle-right"></i><strong> </strong><strong>Single nucleotide</strong> resolution</li> <li><i class="fa fa-arrow-circle-right"></i><strong> Gene-specific </strong>and <strong>genome-wide</strong><span> analyses</span></li> <li><i class="fa fa-arrow-circle-right"></i><strong> NGS</strong><span> </span>compatible</li> </ul> <h2>Downstream analysis techniques</h2> <ul class="square"> <li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit V2</a></li> <li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li> </ul> <p></p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bisulfite-conversion', 'cookies_tag_id' => null, 'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing', 'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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It enables <span>to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by <strong>PCR amplification</strong> or <strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <h2>How it works</h2> <p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged. <span>The DNA is then amplified by PCR where the uracils are converted to thymines. </span></p> <p style="text-align: center;"><span></span></p> <p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p> <h2>Advantages</h2> <ul class="nobullet" style="font-size: 19px;"> <li><i class="fa fa-arrow-circle-right"></i><strong> </strong><strong>Single nucleotide</strong> resolution</li> <li><i class="fa fa-arrow-circle-right"></i><strong> Gene-specific </strong>and <strong>genome-wide</strong><span> analyses</span></li> <li><i class="fa fa-arrow-circle-right"></i><strong> NGS</strong><span> </span>compatible</li> </ul> <h2>Downstream analysis techniques</h2> <ul class="square"> <li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit V2</a></li> <li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li> </ul> <p></p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bisulfite-conversion', 'cookies_tag_id' => null, 'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing', 'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. 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