Alternative names: TRX2, WBP7, MLL4
Polyclonal antibody raised in rabbit against mouse Mll2 (Myeloid/lymphoid or mixed-lineage leukemia protein 2), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal and one peptide containing a sequence from the central part of the protein, respectively.
| Lot | A268-004 |
|---|---|
| Concentration | Not determined |
| Species reactivity | Mouse: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Whole antiserum from rabbit containing 0.05% azide. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:200 - 1:2,000 | Fig 1 |
| Western Blotting | 1:500 | Fig 2, (1) |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Mll2 (Cat. No. CS-099-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:32,500.
Figure 2. Western blot analysis using the Diagenode antibody directed against Mll2
Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Mll2 (Cat. No. CS-099-100), diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of Mll2 is indicated on the right (270 kDa). Cells homozygous for the targeted conditional mll2 allele (ΔMll2) show a dramatic reduction of Mll2 protein after partial recombination, whereas Mll2 protein was detected in ΔMll1, ΔMll4 and WT E14Tg2a cells.
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