| Datasheet Mouse GAPDH promoter pp1045 DATASHEET Datasheet description | Download |
The primer pair Cat. No. C17021045 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from mouse. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).
| Datasheet Mouse GAPDH promoter pp1045 DATASHEET Datasheet description | Download |
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mGlu1 Receptors Monopolize the Synaptic Control of Cerebellar Purkinje Cells by Epigenetically Down-Regulating mGlu5 Receptors. |
High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development |
Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse |
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The cognate mGlu5 receptor is absent in mature PCs and shows low expression levels in the adult cerebellar cortex. Here we found that mGlu5 receptors were heavily expressed by PCs in the early postnatal life, when mGlu1α receptors were barely detectable. The developmental decline of mGlu5 receptors coincided with the appearance of mGlu1α receptors in PCs, and both processes were associated with specular changes in CpG methylation in the corresponding gene promoters. It was the mGlu1 receptor that drove the elimination of mGlu5 receptors from PCs, as shown by data obtained with conditional mGlu1α receptor knockout mice and with targeted pharmacological treatments during critical developmental time windows. The suppressing activity of mGlu1 receptors on mGlu5 receptor was maintained in mature PCs, suggesting that expression of mGlu1α and mGlu5 receptors is mutually exclusive in PCs. 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HoxA5 is a member of Hox gene family playing a central role during axial body patterning and morphogenesis. DNA modification studies have shown that the function of Hox genes is partly governed by the methylation-mediated gene expression regulation. Therefore the study aimed to investigate the role of epigenetic events in regulation of tissue-specific expression pattern of HoxA5 gene during mammalian development. The methodology adopted were sodium bisulfite genomic DNA sequencing, quantitative real-time PCR and chromatin-immunoprecipitation (ChIP). Methylation profiling of HoxA5 gene promoter shows higher methylation in adult as compared to fetus in various somatic tissues of mouse being highest in adult spleen. However q-PCR results show higher expression during fetal stages being highest in fetal intestine followed by brain, liver and spleen. These results clearly indicate a strict correlation between DNA methylation and tissue-specific gene expression. The findings of chromatin-immunoprecipitation (ChIP) have also reinforced that epigenetic event like DNA methylation plays important role in the regulation of tissue specific expression of HoxA5.</p>', 'date' => '2017-01-02', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/28363633', 'doi' => '', 'modified' => '2017-05-22 09:48:38', 'created' => '2017-05-22 09:48:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2979', 'name' => 'Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse', 'authors' => 'Pamnani M et al.', 'description' => '<p>Sox9 and Oct4 are two important regulatory factors involved in mammalian development. Sox9, a member of the group E Sox transcription factor family, has a crucial role in the development of the genitourinary system, while Oct4, commonly known as octamer binding transcription factor 4, belongs to class V of the transcription family. The expression of these two proteins exhibits a dynamic pattern with regard to their expression sites and levels. The aim of this study was to investigate the role of de novo methylation in the regulation of the tissue- and site-specific expression of these proteins. The dynamics of the de novo methylation of 15 CpGs and six CpGs in Sox9 and Oct4 respectively, was studied with sodium bisulfite genomic DNA sequencing in mouse testis at different developmental stages. Consistent methylation of three CpGs was observed in adult ovary in which the expression of Sox9 was feeble, while the level of methylation in somatic tissue was greater in Oct4 compared to germinal tissue. The promoter-chromatin status of Sox9 was also studied with a chromatin immune-precipitation assay.</p>', 'date' => '2016-07-04', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27381637', 'doi' => '10.1590/1678-4685-GMB-2015-0172', 'modified' => '2016-07-11 12:31:08', 'created' => '2016-07-11 12:31:08', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/mouse-gapdh-promoter-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2596', 'antibody_id' => null, 'name' => 'Mouse GAPDH promoter primer pair', 'description' => '<p><span>The primer pair cat # pp-1044-050, 500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from mouse. 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Sox9, a member of the group E Sox transcription factor family, has a crucial role in the development of the genitourinary system, while Oct4, commonly known as octamer binding transcription factor 4, belongs to class V of the transcription family. The expression of these two proteins exhibits a dynamic pattern with regard to their expression sites and levels. The aim of this study was to investigate the role of de novo methylation in the regulation of the tissue- and site-specific expression of these proteins. The dynamics of the de novo methylation of 15 CpGs and six CpGs in Sox9 and Oct4 respectively, was studied with sodium bisulfite genomic DNA sequencing in mouse testis at different developmental stages. Consistent methylation of three CpGs was observed in adult ovary in which the expression of Sox9 was feeble, while the level of methylation in somatic tissue was greater in Oct4 compared to germinal tissue. The promoter-chromatin status of Sox9 was also studied with a chromatin immune-precipitation assay.</p>', 'date' => '2016-07-04', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27381637', 'doi' => '10.1590/1678-4685-GMB-2015-0172', 'modified' => '2016-07-11 12:31:08', 'created' => '2016-07-11 12:31:08', 'ProductsPublication' => array( 'id' => '1427', 'product_id' => '2595', 'publication_id' => '2979' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/27381637" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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The cognate mGlu5 receptor is absent in mature PCs and shows low expression levels in the adult cerebellar cortex. Here we found that mGlu5 receptors were heavily expressed by PCs in the early postnatal life, when mGlu1α receptors were barely detectable. The developmental decline of mGlu5 receptors coincided with the appearance of mGlu1α receptors in PCs, and both processes were associated with specular changes in CpG methylation in the corresponding gene promoters. It was the mGlu1 receptor that drove the elimination of mGlu5 receptors from PCs, as shown by data obtained with conditional mGlu1α receptor knockout mice and with targeted pharmacological treatments during critical developmental time windows. The suppressing activity of mGlu1 receptors on mGlu5 receptor was maintained in mature PCs, suggesting that expression of mGlu1α and mGlu5 receptors is mutually exclusive in PCs. These findings add complexity to the the finely tuned mechanisms that regulate PC biology during development and in the adult life and lay the groundwork for an in-depth analysis of the role played by mGlu5 receptors in PC maturation.</p>', 'date' => '2018-09-06', 'pmid' => 'http://www.pubmed.gov/30190524', 'doi' => '10.1038/s41598-018-31369-7', 'modified' => '2018-12-31 11:36:04', 'created' => '2018-12-04 09:51:07', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3183', 'name' => 'High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development', 'authors' => 'Sinha P. et al.', 'description' => '<p>Homeobox genes encode a group of DNA binding regulatory proteins whose key function occurs in the spatial-temporal organization of genome during embryonic development and differentiation. The role of these Hox genes during ontogenesis makes it an important model for research. HoxA5 is a member of Hox gene family playing a central role during axial body patterning and morphogenesis. DNA modification studies have shown that the function of Hox genes is partly governed by the methylation-mediated gene expression regulation. Therefore the study aimed to investigate the role of epigenetic events in regulation of tissue-specific expression pattern of HoxA5 gene during mammalian development. The methodology adopted were sodium bisulfite genomic DNA sequencing, quantitative real-time PCR and chromatin-immunoprecipitation (ChIP). Methylation profiling of HoxA5 gene promoter shows higher methylation in adult as compared to fetus in various somatic tissues of mouse being highest in adult spleen. However q-PCR results show higher expression during fetal stages being highest in fetal intestine followed by brain, liver and spleen. These results clearly indicate a strict correlation between DNA methylation and tissue-specific gene expression. The findings of chromatin-immunoprecipitation (ChIP) have also reinforced that epigenetic event like DNA methylation plays important role in the regulation of tissue specific expression of HoxA5.</p>', 'date' => '2017-01-02', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/28363633', 'doi' => '', 'modified' => '2017-05-22 09:48:38', 'created' => '2017-05-22 09:48:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2979', 'name' => 'Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse', 'authors' => 'Pamnani M et al.', 'description' => '<p>Sox9 and Oct4 are two important regulatory factors involved in mammalian development. 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The findings of chromatin-immunoprecipitation (ChIP) have also reinforced that epigenetic event like DNA methylation plays important role in the regulation of tissue specific expression of HoxA5.</p>', 'date' => '2017-01-02', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/28363633', 'doi' => '', 'modified' => '2017-05-22 09:48:38', 'created' => '2017-05-22 09:48:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2979', 'name' => 'Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse', 'authors' => 'Pamnani M et al.', 'description' => '<p>Sox9 and Oct4 are two important regulatory factors involved in mammalian development. 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The cognate mGlu5 receptor is absent in mature PCs and shows low expression levels in the adult cerebellar cortex. Here we found that mGlu5 receptors were heavily expressed by PCs in the early postnatal life, when mGlu1α receptors were barely detectable. The developmental decline of mGlu5 receptors coincided with the appearance of mGlu1α receptors in PCs, and both processes were associated with specular changes in CpG methylation in the corresponding gene promoters. It was the mGlu1 receptor that drove the elimination of mGlu5 receptors from PCs, as shown by data obtained with conditional mGlu1α receptor knockout mice and with targeted pharmacological treatments during critical developmental time windows. The suppressing activity of mGlu1 receptors on mGlu5 receptor was maintained in mature PCs, suggesting that expression of mGlu1α and mGlu5 receptors is mutually exclusive in PCs. These findings add complexity to the the finely tuned mechanisms that regulate PC biology during development and in the adult life and lay the groundwork for an in-depth analysis of the role played by mGlu5 receptors in PC maturation.</p>', 'date' => '2018-09-06', 'pmid' => 'http://www.pubmed.gov/30190524', 'doi' => '10.1038/s41598-018-31369-7', 'modified' => '2018-12-31 11:36:04', 'created' => '2018-12-04 09:51:07', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3183', 'name' => 'High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development', 'authors' => 'Sinha P. et al.', 'description' => '<p>Homeobox genes encode a group of DNA binding regulatory proteins whose key function occurs in the spatial-temporal organization of genome during embryonic development and differentiation. The role of these Hox genes during ontogenesis makes it an important model for research. HoxA5 is a member of Hox gene family playing a central role during axial body patterning and morphogenesis. DNA modification studies have shown that the function of Hox genes is partly governed by the methylation-mediated gene expression regulation. Therefore the study aimed to investigate the role of epigenetic events in regulation of tissue-specific expression pattern of HoxA5 gene during mammalian development. The methodology adopted were sodium bisulfite genomic DNA sequencing, quantitative real-time PCR and chromatin-immunoprecipitation (ChIP). Methylation profiling of HoxA5 gene promoter shows higher methylation in adult as compared to fetus in various somatic tissues of mouse being highest in adult spleen. However q-PCR results show higher expression during fetal stages being highest in fetal intestine followed by brain, liver and spleen. These results clearly indicate a strict correlation between DNA methylation and tissue-specific gene expression. The findings of chromatin-immunoprecipitation (ChIP) have also reinforced that epigenetic event like DNA methylation plays important role in the regulation of tissue specific expression of HoxA5.</p>', 'date' => '2017-01-02', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/28363633', 'doi' => '', 'modified' => '2017-05-22 09:48:38', 'created' => '2017-05-22 09:48:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2979', 'name' => 'Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse', 'authors' => 'Pamnani M et al.', 'description' => '<p>Sox9 and Oct4 are two important regulatory factors involved in mammalian development. Sox9, a member of the group E Sox transcription factor family, has a crucial role in the development of the genitourinary system, while Oct4, commonly known as octamer binding transcription factor 4, belongs to class V of the transcription family. The expression of these two proteins exhibits a dynamic pattern with regard to their expression sites and levels. The aim of this study was to investigate the role of de novo methylation in the regulation of the tissue- and site-specific expression of these proteins. The dynamics of the de novo methylation of 15 CpGs and six CpGs in Sox9 and Oct4 respectively, was studied with sodium bisulfite genomic DNA sequencing in mouse testis at different developmental stages. Consistent methylation of three CpGs was observed in adult ovary in which the expression of Sox9 was feeble, while the level of methylation in somatic tissue was greater in Oct4 compared to germinal tissue. The promoter-chromatin status of Sox9 was also studied with a chromatin immune-precipitation assay.</p>', 'date' => '2016-07-04', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27381637', 'doi' => '10.1590/1678-4685-GMB-2015-0172', 'modified' => '2016-07-11 12:31:08', 'created' => '2016-07-11 12:31:08', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/mouse-gapdh-promoter-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2596', 'antibody_id' => null, 'name' => 'Mouse GAPDH promoter primer pair', 'description' => '<p><span>The primer pair cat # pp-1044-050, 500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from mouse. 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It includes control DNA and primers to assess the effiency of the assay. </span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</p> <p><em>Looking for hMeDIP-seq protocol? <a href="https://go.diagenode.com/l/928883/2022-01-07/2m1ht" target="_blank" title="Contact us">Contact us</a></em></p> <p><span></span></p> <p><span></span></p>', 'label1' => 'Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>hmeDNA and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010031', 'old_catalog_number' => 'AF-110-0016', 'sf_code' => 'C02010031-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2023-04-20 16:12:48', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '4711', 'product_id' => '2595', 'related_id' => '1882' ), 'Image' => array( (int) 0 => array( 'id' => '1778', 'name' => 'product/kits/methyl-kit-icon.png', 'alt' => 'Methylation kit icon', 'modified' => '2019-04-23 15:17:01', 'created' => '2018-03-15 15:52:12', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1045-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2596', 'antibody_id' => null, 'name' => 'Mouse GAPDH promoter primer pair', 'description' => '<p><span>The primer pair cat # pp-1044-050, 500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from mouse. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17021045-500', 'old_catalog_number' => 'pp-1045-500', 'sf_code' => 'C17021045-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mouse-gapdh-promoter-primer-pair-500-ul', 'meta_title' => 'Mouse GAPDH promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Mouse GAPDH promoter primer pair', 'modified' => '2016-02-19 22:13:36', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '162', 'product_id' => '2596', 'group_id' => '151' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '248', 'name' => 'Datasheet Mouse GAPDH promoter pp1045', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Mouse_GAPDH_promoter_pp1045.pdf', 'slug' => 'datasheet-mouse-gapdh-promoter-pp1045', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '765', 'product_id' => '2595', 'document_id' => '248' ) ) $publication = array( 'id' => '2979', 'name' => 'Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse', 'authors' => 'Pamnani M et al.', 'description' => '<p>Sox9 and Oct4 are two important regulatory factors involved in mammalian development. Sox9, a member of the group E Sox transcription factor family, has a crucial role in the development of the genitourinary system, while Oct4, commonly known as octamer binding transcription factor 4, belongs to class V of the transcription family. The expression of these two proteins exhibits a dynamic pattern with regard to their expression sites and levels. The aim of this study was to investigate the role of de novo methylation in the regulation of the tissue- and site-specific expression of these proteins. The dynamics of the de novo methylation of 15 CpGs and six CpGs in Sox9 and Oct4 respectively, was studied with sodium bisulfite genomic DNA sequencing in mouse testis at different developmental stages. Consistent methylation of three CpGs was observed in adult ovary in which the expression of Sox9 was feeble, while the level of methylation in somatic tissue was greater in Oct4 compared to germinal tissue. The promoter-chromatin status of Sox9 was also studied with a chromatin immune-precipitation assay.</p>', 'date' => '2016-07-04', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27381637', 'doi' => '10.1590/1678-4685-GMB-2015-0172', 'modified' => '2016-07-11 12:31:08', 'created' => '2016-07-11 12:31:08', 'ProductsPublication' => array( 'id' => '1427', 'product_id' => '2595', 'publication_id' => '2979' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/27381637" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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