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Polyclonal antibody raised in rabbit against Myc (v-myc myelocytomatosis viral oncogene homolog) acetylated at lysine 323 (MycK323ac) using a KLH-conjugated synthetic peptide.
Lot
A506-0041
Concentration
0.8 μg/μl
Species reactivity
Human: positive. Other species: not tested.
Type
Polyclonal
Purity
Affinity purified polyclonal antibody.
Host
Rabbit
Storage Conditions
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer
PBS containing 0.05% azide and 0.05% ProClin 300.
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MycK323ac (Cat. No. C15410346). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,000.
Figure 2. Immunofluorescence using the Diagenode antibody directed against MycK323ac HeLa cells were stained with the Diagenode antibody against MycK323ac (cat. No. C15410346) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the MycK323ac antibody (middle) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
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