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Polyclonal antibody raised in rabbit against OCT4 (Octamer-Binding Protein 4), using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.
| Lot | A2089P |
|---|---|
| Concentration | 1.5 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 5 μg/ChIP | Fig 1, 2 |
| ELISA | 1:1,000 - 1:10,000 | Fig 3 |
| Western Blotting | 1:1,000 | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4
ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4
ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D).
Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000.
Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4
Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ELISA Enzyme-linked immunosorbent assay. Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Datasheet OCT4 C15410305 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: OCT4 Antibody (sample size) (Diagenode Cat# C15410305-10 Lot# A2089P). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal. |
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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. 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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. 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Batch-specific data available on the website.', 'modified' => '2021-12-23 12:22:44', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '512', 'name' => 'Datasheet OCT4 C15410305', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_OCT4_C15410305.pdf', 'slug' => 'datasheet-oct4-c15410305', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2959', 'name' => 'Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.', 'authors' => 'Holm F et al.', 'description' => '<p>Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2318', 'name' => 'OCT4 Antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2320', 'name' => 'OCT4 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2315', 'name' => 'OCT4 Antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2319', 'name' => 'OCT4 Antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-JP-ja-GHS_1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2314', 'name' => 'OCT4 Antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2313', 'name' => 'OCT4 Antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2317', 'name' => 'OCT4 Antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2316', 'name' => 'OCT4 Antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/oct4-polylconal-antibody-pioneer-50-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. 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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. 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In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( 'id' => '4831', 'product_id' => '3148', 'publication_id' => '2959' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26621726" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. 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A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15410305-10', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:22:56', 'created' => '2020-11-25 16:42:01', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '622', 'name' => 'OCT4 Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'A2089P', 'concentration' => '1.5 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:1,000 - 1:10,000</td> <td>Fig 3</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-25 16:39:10', 'created' => '2020-11-25 16:39:10', 'select_label' => '622 - OCT4 Antibody - ChIP-seq Grade (sample size) (A2089P - 1.5 µg/µl - Human - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '327', 'name' => 'C15410305', 'product_id' => '2424', 'modified' => '2020-11-25 16:42:21', 'created' => '2020-11-25 16:42:21' ), 'Master' => array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website.', 'modified' => '2021-12-23 12:22:44', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '512', 'name' => 'Datasheet OCT4 C15410305', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_OCT4_C15410305.pdf', 'slug' => 'datasheet-oct4-c15410305', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2959', 'name' => 'Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.', 'authors' => 'Holm F et al.', 'description' => '<p>Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. 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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. 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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. 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In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( 'id' => '4831', 'product_id' => '3148', 'publication_id' => '2959' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26621726" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'product' => array( 'Product' => array( 'id' => '3148', 'antibody_id' => '622', 'name' => 'OCT4 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15410305-10', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:22:56', 'created' => '2020-11-25 16:42:01', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '622', 'name' => 'OCT4 Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'A2089P', 'concentration' => '1.5 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:1,000 - 1:10,000</td> <td>Fig 3</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-25 16:39:10', 'created' => '2020-11-25 16:39:10', 'select_label' => '622 - OCT4 Antibody - ChIP-seq Grade (sample size) (A2089P - 1.5 µg/µl - Human - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/oct4-polylconal-antibody-pioneer-50-mg' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3148', 'antibody_id' => '622', 'name' => 'OCT4 Antibody (sample size)', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15410305-10', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:22:56', 'created' => '2020-11-25 16:42:01', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '622', 'name' => 'OCT4 Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'A2089P', 'concentration' => '1.5 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:1,000 - 1:10,000</td> <td>Fig 3</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-25 16:39:10', 'created' => '2020-11-25 16:39:10', 'select_label' => '622 - OCT4 Antibody - ChIP-seq Grade (sample size) (A2089P - 1.5 µg/µl - Human - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '327', 'name' => 'C15410305', 'product_id' => '2424', 'modified' => '2020-11-25 16:42:21', 'created' => '2020-11-25 16:42:21' ), 'Master' => array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. 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Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '512', 'name' => 'Datasheet OCT4 C15410305', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_OCT4_C15410305.pdf', 'slug' => 'datasheet-oct4-c15410305', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2959', 'name' => 'Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.', 'authors' => 'Holm F et al.', 'description' => '<p>Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2318', 'name' => 'OCT4 Antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2320', 'name' => 'OCT4 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2315', 'name' => 'OCT4 Antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2319', 'name' => 'OCT4 Antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-JP-ja-GHS_1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2314', 'name' => 'OCT4 Antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2313', 'name' => 'OCT4 Antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2317', 'name' => 'OCT4 Antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2316', 'name' => 'OCT4 Antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/oct4-polylconal-antibody-pioneer-50-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website.', 'modified' => '2021-12-23 12:22:44', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3148', 'antibody_id' => '622', 'name' => 'OCT4 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15410305-10', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:22:56', 'created' => '2020-11-25 16:42:01', 'ProductsGroup' => array( 'id' => '367', 'product_id' => '3148', 'group_id' => '327' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. 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In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( 'id' => '4831', 'product_id' => '3148', 'publication_id' => '2959' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26621726" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15410305-10', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:22:56', 'created' => '2020-11-25 16:42:01', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '622', 'name' => 'OCT4 Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'A2089P', 'concentration' => '1.5 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:1,000 - 1:10,000</td> <td>Fig 3</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-25 16:39:10', 'created' => '2020-11-25 16:39:10', 'select_label' => '622 - OCT4 Antibody - ChIP-seq Grade (sample size) (A2089P - 1.5 µg/µl - Human - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '327', 'name' => 'C15410305', 'product_id' => '2424', 'modified' => '2020-11-25 16:42:21', 'created' => '2020-11-25 16:42:21' ), 'Master' => array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website.', 'modified' => '2021-12-23 12:22:44', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '512', 'name' => 'Datasheet OCT4 C15410305', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_OCT4_C15410305.pdf', 'slug' => 'datasheet-oct4-c15410305', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2959', 'name' => 'Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.', 'authors' => 'Holm F et al.', 'description' => '<p>Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2318', 'name' => 'OCT4 Antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2320', 'name' => 'OCT4 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2315', 'name' => 'OCT4 Antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2319', 'name' => 'OCT4 Antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-JP-ja-GHS_1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2314', 'name' => 'OCT4 Antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2313', 'name' => 'OCT4 Antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2317', 'name' => 'OCT4 Antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2316', 'name' => 'OCT4 Antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/oct4-polylconal-antibody-pioneer-50-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website.', 'modified' => '2021-12-23 12:22:44', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3148', 'antibody_id' => '622', 'name' => 'OCT4 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15410305-10', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'oct4-polylconal-antibody-pioneer-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:22:56', 'created' => '2020-11-25 16:42:01', 'ProductsGroup' => array( 'id' => '367', 'product_id' => '3148', 'group_id' => '327' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2424', 'antibody_id' => '407', 'name' => 'OCT4 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against <strong>OCT4 (Octamer-Binding Protein 4)</strong>, using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chip.png" alt="OCT4 Antibody ChIP Grade" caption="false" width="288" height="209" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig1.png" alt="OCT4 Antibody ChIP-seq Grade" caption="false" width="288" height="34" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig2.png" alt="OCT4 Antibody for ChIP-seq" caption="false" width="288" height="52" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig3.png" alt="OCT4 Antibody for ChIP-seq assay" caption="false" width="288" height="38" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-chipseq-fig4.png" alt="OCT4 Antibody validated in ChIP-seq" caption="false" width="288" height="57" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4</strong><br /> ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-ELISA.png" alt="OCT4 Antibody for ELISA validation" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410305-WB.png" alt="OCT4 Antibody validated in Western Blot" caption="false" width="123" height="167" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4</strong><br /> Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>OCT4 (UniProtKB/Swiss-Prot entry Q01860) is a transcription factor that plays a key role in embryonic development and stem cell pluripotency. During embryonic development OCT4 forms a trimeric complex with SOX2 and controls the expression of a number of genes important for embryogenesis such as YES1, FGF4, UTF1 and ZFP206. Aberrant expression of OCT4 in adult tissues is associated with tumorigenesis. A translocation with the Ewing’s sarcoma gene on chromosome 21 also leads to tumor formation.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410305', 'old_catalog_number' => '', 'sf_code' => 'C15410305-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'oct4-polylconal-antibody-pioneer-50-mg', 'meta_title' => 'OCT4 Antibody - ChIP-seq Grade (C15410305) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'OCT4 (Octamer-Binding Protein 4) Polylconal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website.', 'modified' => '2021-12-23 12:22:44', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '5359', 'product_id' => '3148', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '512', 'name' => 'Datasheet OCT4 C15410305', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_OCT4_C15410305.pdf', 'slug' => 'datasheet-oct4-c15410305', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '3023', 'product_id' => '3148', 'document_id' => '512' ) ) $sds = array( 'id' => '2316', 'name' => 'OCT4 Antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/OCT4/SDS-C15410305-OCT4_Antibody-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 11:57:21', 'created' => '2022-08-29 11:57:21', 'ProductsSafetySheet' => array( 'id' => '4007', 'product_id' => '3148', 'safety_sheet_id' => '2316' ) ) $publication = array( 'id' => '2959', 'name' => 'Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.', 'authors' => 'Holm F et al.', 'description' => '<p>Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination.</p>', 'date' => '2015-12-15', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26621726', 'doi' => ' 10.1073/pnas.1506943112', 'modified' => '2016-06-23 10:25:24', 'created' => '2016-06-23 10:25:24', 'ProductsPublication' => array( 'id' => '4831', 'product_id' => '3148', 'publication_id' => '2959' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26621726" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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