| Datasheet p53 sp083 DATASHEET Datasheet description | Download |
| Datasheet p53 - C15410083 DATASHEET Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugate... | Download |
Synthetic peptide corresponding to the C-terminal region of human p53.
This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments.
For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).
| Datasheet p53 sp083 DATASHEET Datasheet description | Download |
| Datasheet p53 - C15410083 DATASHEET Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugate... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: p53 peptide (Diagenode Cat# C16000083). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'meta_title' => 'p53 peptide', 'product' => array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'p53 peptide' $meta_title = 'p53 peptide' $product = array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. 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ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '358', 'name' => 'Datasheet p53 sp083', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_sp083.pdf', 'slug' => 'datasheet-p53-sp083', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/p53-polyclonal-antibody-classic-50-ug-50-ul"><img src="/img/product/antibodies/chipseq-grade-ab-icon.png" alt="ChIP-seq Grade" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15410083</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2240" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2240" id="CartAdd/2240Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2240" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> p53 Antibody</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="p53-polyclonal-antibody-classic-50-ug-50-ul" data-reveal-id="cartModal-2240" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">p53 Antibody</h6> </div> </div> </li> ' $related = array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '1961', 'product_id' => '2461', 'related_id' => '2240' ), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-083-050)</span></small>' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '822', 'product_id' => '2461', 'document_id' => '644' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'meta_title' => 'p53 peptide', 'product' => array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'p53 peptide' $meta_title = 'p53 peptide' $product = array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '358', 'name' => 'Datasheet p53 sp083', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_sp083.pdf', 'slug' => 'datasheet-p53-sp083', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/p53-polyclonal-antibody-classic-50-ug-50-ul"><img src="/img/product/antibodies/chipseq-grade-ab-icon.png" alt="ChIP-seq Grade" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15410083</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2240" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2240" id="CartAdd/2240Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2240" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> p53 Antibody</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="p53-polyclonal-antibody-classic-50-ug-50-ul" data-reveal-id="cartModal-2240" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">p53 Antibody</h6> </div> </div> </li> ' $related = array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '1961', 'product_id' => '2461', 'related_id' => '2240' ), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-083-050)</span></small>' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '822', 'product_id' => '2461', 'document_id' => '644' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'meta_title' => 'p53 peptide', 'product' => array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'p53 peptide' $meta_title = 'p53 peptide' $product = array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '358', 'name' => 'Datasheet p53 sp083', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_sp083.pdf', 'slug' => 'datasheet-p53-sp083', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/p53-polyclonal-antibody-classic-50-ug-50-ul"><img src="/img/product/antibodies/chipseq-grade-ab-icon.png" alt="ChIP-seq Grade" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15410083</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2240" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2240" id="CartAdd/2240Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2240" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> p53 Antibody</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="p53-polyclonal-antibody-classic-50-ug-50-ul" data-reveal-id="cartModal-2240" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">p53 Antibody</h6> </div> </div> </li> ' $related = array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '1961', 'product_id' => '2461', 'related_id' => '2240' ), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-083-050)</span></small>' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '822', 'product_id' => '2461', 'document_id' => '644' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'meta_title' => 'p53 peptide', 'product' => array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'p53 peptide' $meta_title = 'p53 peptide' $product = array( 'Product' => array( 'id' => '2461', 'antibody_id' => null, 'name' => 'p53 peptide', 'description' => '<p><span>Synthetic peptide corresponding to the C-terminal region of human p53. </span><br /><span>This peptide was used as immunogen to raise the Diagenode ChIP grade antibody directed against p53 (Cat. No. pAb-083-050). The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about p53: see overview in the Technical Data Sheet of the corresponding Diagenode antibody (Cat. No. pAb-083-050).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000083', 'old_catalog_number' => 'sp-083-050', 'sf_code' => 'C16000083-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-peptide-50-ug-50-ul', 'meta_title' => 'p53 peptide', 'meta_keywords' => '', 'meta_description' => 'p53 peptide', 'modified' => '2015-07-27 10:55:31', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. Sample size available.', 'modified' => '2021-12-23 12:22:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '358', 'name' => 'Datasheet p53 sp083', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_sp083.pdf', 'slug' => 'datasheet-p53-sp083', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '644', 'name' => 'Datasheet p53 - C15410083', 'description' => '<p class="p1">Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_p53_pAb-083-050.pdf', 'slug' => 'datasheet-p53-pab-083-050', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-08-09 11:18:09', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/p53-polyclonal-antibody-classic-50-ug-50-ul"><img src="/img/product/antibodies/chipseq-grade-ab-icon.png" alt="ChIP-seq Grade" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15410083</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2240" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2240" id="CartAdd/2240Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2240" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> p53 Antibody</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('p53 Antibody', 'C15410083', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="p53-polyclonal-antibody-classic-50-ug-50-ul" data-reveal-id="cartModal-2240" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">p53 Antibody</h6> </div> </div> </li> ' $related = array( 'id' => '2240', 'antibody_id' => '312', 'name' => 'p53 Antibody', 'description' => '<p><span>Alternative names: <strong>TP53</strong>, <strong>P53</strong>, <strong>TRP53</strong>, <strong>LSF1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human <strong>p53 (tumor protein p53)</strong>, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083-chip.jpg" alt="p53 Antibody ChIP Grade" caption="false" width="400" height="304" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP assays were performed using human U2OS cells, treated with camptothecin, the Diagenode antibody against p53 (Cat. No. C15410083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. qPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-A.jpg" alt="p53 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-B.jpg" alt="p53 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-C.jpg" alt="p53 Antibody for ChIP-seq assay " style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410083_ChIPSeq-D.jpg" alt="p53 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against p53</strong><br /> ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 µg of the Diagenode antibody against p53 (Cat. No. C15410083) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_ELISA.jpg" alt="p53 Antibody ELISA validation " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human p53 (Cat. No. C15410083), in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:308,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410083_WB.jpg" alt="p53 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against p53</strong><br /> Nuclear extracts of HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against p53 (Cat. No. C15410083) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>The transcription factor p53 (UniProt/Swiss-Prot entry P04637) is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg / 28 µl', 'catalog_number' => 'C15410083', 'old_catalog_number' => 'pAb-083-050', 'sf_code' => 'C15410083-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'p53-polyclonal-antibody-classic-50-ug-50-ul', 'meta_title' => 'p53 Antibody - ChIP-seq Grade (C15410083) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'p53 (Tumor protein p53) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Alternative names: TP53, P53, TRP53, LSF1. 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