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Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5
Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.
| Lot | A63-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:1,000 - 1:5,000 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.
Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet PADI4 CS-075-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: PADI4 Antibody (Diagenode Cat# C15310075 Lot# A63-001 ). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.', 'meta_title' => 'PADI4 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. 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PADI4 also citrullinates EP300/P300 at ‘Arg-2142’, which favours its interaction with NCOA2/ GRIP.', 'clonality' => '', 'isotype' => '', 'lot' => 'A63-001 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000 - 1:5,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.' $meta_title = 'PADI4 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310075', 'old_catalog_number' => 'CS-075-100', 'sf_code' => 'C15310075-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'padi4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'PADI4 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:02:32', 'created' => '0000-00-00 00:00:00', 'select_label' => '209 - PADI4 polyclonal antibody (A63-001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ) ), 'Group' => array( 'Group' => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ), 'Master' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '430', 'name' => 'Datasheet PADI4 CS-075-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_PADI4_CS-075-100.pdf', 'slug' => 'datasheet-padi4-cs-075-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.', 'meta_title' => 'PADI4 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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PADI4 also citrullinates EP300/P300 at ‘Arg-2142’, which favours its interaction with NCOA2/ GRIP.', 'clonality' => '', 'isotype' => '', 'lot' => 'A63-001 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000 - 1:5,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:02:32', 'created' => '0000-00-00 00:00:00', 'select_label' => '209 - PADI4 polyclonal antibody (A63-001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/padi4-polyclonal-antibody-pioneer-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.' $meta_title = 'PADI4 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310075', 'old_catalog_number' => 'CS-075-100', 'sf_code' => 'C15310075-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'padi4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'PADI4 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.', 'modified' => '2022-01-05 15:53:57', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '209', 'name' => 'PADI4 polyclonal antibody', 'description' => 'Protein arginine deiminases catalyse the conversion of arginine residues in proteins to citrulline. PADI4 (UniProt/ Swiss-Prot entry Q9UM07) catalyses the citrullination-deimination of Arg-8 and Arg-17 from histone H3 and Arg-3 from histone H4. This prevents their methylation by CARM1 and HRMT1L2/PRMT1, thereby repressing transcription. PADI4 also citrullinates EP300/P300 at ‘Arg-2142’, which favours its interaction with NCOA2/ GRIP.', 'clonality' => '', 'isotype' => '', 'lot' => 'A63-001 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000 - 1:5,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:02:32', 'created' => '0000-00-00 00:00:00', 'select_label' => '209 - PADI4 polyclonal antibody (A63-001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ) ), 'Group' => array( 'Group' => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ), 'Master' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.', 'modified' => '2022-01-05 15:53:57', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. 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These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Sample size available', 'modified' => '2022-01-05 15:54:10', 'created' => '2019-07-18 14:59:17', 'ProductsGroup' => array( 'id' => '300', 'product_id' => '3033', 'group_id' => '265' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/0-1-ml-bioruptor-microtubes-120-tubes"><img src="/img/product/shearing_technologies/C30010015_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010015</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2629" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/2629" id="CartAdd/2629Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2629" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.1 ml Bioruptor<sup>®</sup> Pico Microtubes</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'C30010015', '225', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'C30010015', '225', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-1-ml-bioruptor-microtubes-120-tubes" data-reveal-id="cartModal-2629" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">0.1 ml Bioruptor® Pico Microtubes</h6> </div> </div> </li> ' $related = array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.', 'meta_title' => 'PADI4 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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PADI4 also citrullinates EP300/P300 at ‘Arg-2142’, which favours its interaction with NCOA2/ GRIP.', 'clonality' => '', 'isotype' => '', 'lot' => 'A63-001 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000 - 1:5,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.' $meta_title = 'PADI4 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:02:32', 'created' => '0000-00-00 00:00:00', 'select_label' => '209 - PADI4 polyclonal antibody (A63-001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ) ), 'Group' => array( 'Group' => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ), 'Master' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'PADI4 (Protein-arginine deiminase type-4) Polyclonal Antibody validated in ELISA and WB. Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.', 'meta_title' => 'PADI4 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. 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PADI4 also citrullinates EP300/P300 at ‘Arg-2142’, which favours its interaction with NCOA2/ GRIP.', 'clonality' => '', 'isotype' => '', 'lot' => 'A63-001 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000 - 1:5,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5. Sample size available.' $meta_title = 'PADI4 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:02:32', 'created' => '0000-00-00 00:00:00', 'select_label' => '209 - PADI4 polyclonal antibody (A63-001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ) ), 'Group' => array( 'Group' => array( 'id' => '265', 'name' => 'C15310075', 'product_id' => '2075', 'modified' => '2019-07-18 14:59:43', 'created' => '2019-07-18 14:59:43' ), 'Master' => array( 'id' => '2075', 'antibody_id' => '209', 'name' => 'PADI4 Antibody', 'description' => '<p><span>Alternative names: <strong>PAD</strong>, <strong>HL-60 PAD</strong>, <strong>PDI4</strong>, <strong>PADI5</strong>, <strong>PDI5</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). 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