Alternative names: PAP, mtPAP
Polyclonal antibody raised in rabbit against human PAPD1 (PAP associated domain containing 1), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
| Lot | A415-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPD1 (Cat. No. CS-132-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:47,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD1
Whole cell extracts of U2OS cells (80 μg) were analysed by Western blot using the Diagenode antibody against PAPD1 (Cat. No. CS-132-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.
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