Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique molecular identifiers (UMI) for library multiplexing with the Premium RRBS kit V2 and other BS-seq applications. The use of UDIs (unique i5 and i7 barcodes) is highly recommended to mitigate errors introduced by read misassignment. The use of UMIs (9-base) is hilghy recommended for quantitave analysis or low-frequency variant detection.
Read more about Diagenode's BS-seq tools.
UMIs incorporate a unique barcode onto each molecule within a given RRBS library. By incorporating individual barcodes on each original DNA fragment, variant alleles present in the original DNA sample (true variants) can be distinguished from errors introduced during library preparation or sequencing.
Figure 1. Premium RRBS V2 construct. Integrated UMIs enable removal of PCR duplicates during the data analysis.
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