| RIP RIP Read more |
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Other names: psU
Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.
| Lot | 001 |
|---|---|
| Concentration | 1.44 µg/µl |
| Species reactivity | Human, other (wide range): positive. |
| Type | Monoclonal |
| Purity | Protein A purified monoclonal antibody. |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% sodium azide. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP* | 5 µg per IP | Fig 1, 2 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.
A. 
B. 
Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU
RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. RIP using the Diagenode monoclonal antibody directed against psU
RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Pseudouridine Monoclonal Antibody datasheet DATASHEET Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA. | Download |
| Pseudouridine antibody SDS US en | Download |
| Pseudouridine antibody SDS GB en | Download |
| Pseudouridine antibody SDS ES es | Download |
| Pseudouridine antibody SDS DE de | Download |
| Pseudouridine antibody SDS JP ja | Download |
| Pseudouridine antibody SDS BE nl | Download |
| Pseudouridine antibody SDS BE fr | Download |
| Pseudouridine antibody SDS FR fr | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Pseudouridine Antibody (Diagenode Cat# C15200247 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis |
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Sample size available.', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'Pseudouridine Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. 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The presence of pseudouridine enhances the structural stability of RNA and is thought to affect rRNA processing, translation and pre-mRNA splicing.', 'clonality' => '', 'isotype' => 'IgG2b', 'lot' => '001', 'concentration' => '1.44 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein A purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>5 µg per IP</td> <td>Fig 1, 2</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'pseudouridine-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-05-15 11:50:26', 'created' => '2019-03-19 14:57:31', 'select_label' => '552 - Pseudouridine monoclonal antibody (001 - 1.44 µg/µl - Human, other (wide range): positive. - Protein A purified monoclonal antibody. - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '261', 'name' => 'C15200247', 'product_id' => '3019', 'modified' => '2019-03-19 15:29:26', 'created' => '2019-03-19 15:29:26' ) ), 'Group' => array( 'Group' => array( 'id' => '261', 'name' => 'C15200247', 'product_id' => '3019', 'modified' => '2019-03-19 15:29:26', 'created' => '2019-03-19 15:29:26' ), 'Master' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1046', 'name' => 'Pseudouridine Monoclonal Antibody datasheet', 'description' => '<p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/pseudouridine-monoclonal-antibody-50-ug.pdf', 'slug' => 'pseudouridine-monoclonal-antibody-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-19 15:40:27', 'created' => '2019-03-19 15:40:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4988', 'name' => 'Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis', 'authors' => 'Xi Wang et al.', 'description' => '<h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Background</h3> <p>Previous studies have indicated that ψ-modified small RNAs play crucial roles in tumor metastasis. However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1584', 'name' => 'Pseudouridine antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1582', 'name' => 'Pseudouridine antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1580', 'name' => 'Pseudouridine antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1579', 'name' => 'Pseudouridine antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1583', 'name' => 'Pseudouridine antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1578', 'name' => 'Pseudouridine antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1577', 'name' => 'Pseudouridine antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1581', 'name' => 'Pseudouridine antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/pseudouridine-monoclonal-antibody-50-ug' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3020', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody (sample size)', 'description' => '', 'label1' => 'Validation data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200247-10', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '0', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'pseudouridine-monoclonal-antibody-10-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( 'id' => '7592', 'product_id' => '3019', 'publication_id' => '4988' ) ) $externalLink = ' <a href="https://link.springer.com/article/10.1186/s12935-024-03482-3" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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The presence of pseudouridine enhances the structural stability of RNA and is thought to affect rRNA processing, translation and pre-mRNA splicing.', 'clonality' => '', 'isotype' => 'IgG2b', 'lot' => '001', 'concentration' => '1.44 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein A purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>5 µg per IP</td> <td>Fig 1, 2</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide.', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'Pseudouridine Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. Batch-specific data available on the website. Sample size available.', 'modified' => '2022-01-05 15:56:31', 'created' => '2019-03-19 15:22:08', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '552', 'name' => 'Pseudouridine monoclonal antibody', 'description' => 'Pseudouridine (psU) was the first modified base that was identified in RNA. It is mainly present in non-coding RNAs such as tRNA, rRNA, snRNA and snoRNA but was also found in some mRNAs in both yeast and human. The presence of pseudouridine enhances the structural stability of RNA and is thought to affect rRNA processing, translation and pre-mRNA splicing.', 'clonality' => '', 'isotype' => 'IgG2b', 'lot' => '001', 'concentration' => '1.44 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein A purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>5 µg per IP</td> <td>Fig 1, 2</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'pseudouridine-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-05-15 11:50:26', 'created' => '2019-03-19 14:57:31', 'select_label' => '552 - Pseudouridine monoclonal antibody (001 - 1.44 µg/µl - Human, other (wide range): positive. - Protein A purified monoclonal antibody. - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '261', 'name' => 'C15200247', 'product_id' => '3019', 'modified' => '2019-03-19 15:29:26', 'created' => '2019-03-19 15:29:26' ) ), 'Group' => array( 'Group' => array( 'id' => '261', 'name' => 'C15200247', 'product_id' => '3019', 'modified' => '2019-03-19 15:29:26', 'created' => '2019-03-19 15:29:26' ), 'Master' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1046', 'name' => 'Pseudouridine Monoclonal Antibody datasheet', 'description' => '<p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/pseudouridine-monoclonal-antibody-50-ug.pdf', 'slug' => 'pseudouridine-monoclonal-antibody-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-19 15:40:27', 'created' => '2019-03-19 15:40:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4988', 'name' => 'Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis', 'authors' => 'Xi Wang et al.', 'description' => '<h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Background</h3> <p>Previous studies have indicated that ψ-modified small RNAs play crucial roles in tumor metastasis. However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1584', 'name' => 'Pseudouridine antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1582', 'name' => 'Pseudouridine antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1580', 'name' => 'Pseudouridine antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1579', 'name' => 'Pseudouridine antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1583', 'name' => 'Pseudouridine antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1578', 'name' => 'Pseudouridine antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1577', 'name' => 'Pseudouridine antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1581', 'name' => 'Pseudouridine antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/pseudouridine-monoclonal-antibody-50-ug' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3020', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody (sample size)', 'description' => '', 'label1' => 'Validation data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200247-10', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '0', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'pseudouridine-monoclonal-antibody-10-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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Sample size available', 'modified' => '2022-01-05 16:17:23', 'created' => '2019-03-19 15:29:12', 'ProductsGroup' => array( 'id' => '296', 'product_id' => '3020', 'group_id' => '261' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '5017', 'product_id' => '3019', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'eng' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '1046', 'name' => 'Pseudouridine Monoclonal Antibody datasheet', 'description' => '<p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/pseudouridine-monoclonal-antibody-50-ug.pdf', 'slug' => 'pseudouridine-monoclonal-antibody-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-19 15:40:27', 'created' => '2019-03-19 15:40:27', 'ProductsDocument' => array( 'id' => '2747', 'product_id' => '3019', 'document_id' => '1046' ) ) $sds = array( 'id' => '1581', 'name' => 'Pseudouridine antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( 'id' => '2752', 'product_id' => '3019', 'safety_sheet_id' => '1581' ) ) $publication = array( 'id' => '4988', 'name' => 'Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis', 'authors' => 'Xi Wang et al.', 'description' => '<h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Background</h3> <p>Previous studies have indicated that ψ-modified small RNAs play crucial roles in tumor metastasis. However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( 'id' => '7592', 'product_id' => '3019', 'publication_id' => '4988' ) ) $externalLink = ' <a href="https://link.springer.com/article/10.1186/s12935-024-03482-3" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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The presence of pseudouridine enhances the structural stability of RNA and is thought to affect rRNA processing, translation and pre-mRNA splicing.', 'clonality' => '', 'isotype' => 'IgG2b', 'lot' => '001', 'concentration' => '1.44 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein A purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>5 µg per IP</td> <td>Fig 1, 2</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'Pseudouridine Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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The presence of pseudouridine enhances the structural stability of RNA and is thought to affect rRNA processing, translation and pre-mRNA splicing.', 'clonality' => '', 'isotype' => 'IgG2b', 'lot' => '001', 'concentration' => '1.44 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein A purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>5 µg per IP</td> <td>Fig 1, 2</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'pseudouridine-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-05-15 11:50:26', 'created' => '2019-03-19 14:57:31', 'select_label' => '552 - Pseudouridine monoclonal antibody (001 - 1.44 µg/µl - Human, other (wide range): positive. - Protein A purified monoclonal antibody. - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '261', 'name' => 'C15200247', 'product_id' => '3019', 'modified' => '2019-03-19 15:29:26', 'created' => '2019-03-19 15:29:26' ) ), 'Group' => array( 'Group' => array( 'id' => '261', 'name' => 'C15200247', 'product_id' => '3019', 'modified' => '2019-03-19 15:29:26', 'created' => '2019-03-19 15:29:26' ), 'Master' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. Batch-specific data available on the website. Sample size available.', 'modified' => '2022-01-05 15:56:31', 'created' => '2019-03-19 15:22:08' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. 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However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( 'id' => '7592', 'product_id' => '3019', 'publication_id' => '4988' ) ) $externalLink = ' <a href="https://link.springer.com/article/10.1186/s12935-024-03482-3" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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The presence of pseudouridine enhances the structural stability of RNA and is thought to affect rRNA processing, translation and pre-mRNA splicing.', 'clonality' => '', 'isotype' => 'IgG2b', 'lot' => '001', 'concentration' => '1.44 µg/µl', 'reactivity' => 'Human, other (wide range): positive.', 'type' => 'Monoclonal', 'purity' => 'Protein A purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP*</td> <td>5 µg per IP</td> <td>Fig 1, 2</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 2-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'pseudouridine-monoclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-05-15 11:50:26', 'created' => '2019-03-19 14:57:31', 'select_label' => '552 - Pseudouridine monoclonal antibody (001 - 1.44 µg/µl - Human, other (wide range): positive. - Protein A purified monoclonal antibody. - Mouse)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/pseudouridine-monoclonal-antibody-50-ug' ) $language = 'en' $meta_keywords = '' $meta_description = 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. Batch-specific data available on the website. Sample size available.' $meta_title = 'Pseudouridine Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '3019', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody', 'description' => '<p><strong>Other names:</strong> psU</p> <p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-1.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15200247-rna-2.png" alt="RNA immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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RNA immunoprecipitation using the Diagenode monoclonal antibody directed against psU</strong><br />RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 5 µg of the Diagenode monoclonal antibody against psU (Cat. No. C15200247) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the psU antibody (right) The left panel shows the input. Figure 1B shows the gel image for the psU antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15200247-rip.png" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. RIP using the Diagenode monoclonal antibody directed against psU</strong><br />RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode antibody against psU (Cat. No. C15200247). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18 and 28s rRNA genes, for the RPL19 and ATP5E mRNA, shown to contain a psU residue and for the MB gene, used as a negative control. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15200247', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 27, 2019', 'slug' => 'pseudouridine-monoclonal-antibody-50-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1046', 'name' => 'Pseudouridine Monoclonal Antibody datasheet', 'description' => '<p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/pseudouridine-monoclonal-antibody-50-ug.pdf', 'slug' => 'pseudouridine-monoclonal-antibody-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-19 15:40:27', 'created' => '2019-03-19 15:40:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4988', 'name' => 'Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis', 'authors' => 'Xi Wang et al.', 'description' => '<h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Background</h3> <p>Previous studies have indicated that ψ-modified small RNAs play crucial roles in tumor metastasis. However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1584', 'name' => 'Pseudouridine antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1582', 'name' => 'Pseudouridine antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1580', 'name' => 'Pseudouridine antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1579', 'name' => 'Pseudouridine antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1583', 'name' => 'Pseudouridine antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1578', 'name' => 'Pseudouridine antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1577', 'name' => 'Pseudouridine antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1581', 'name' => 'Pseudouridine antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/pseudouridine-monoclonal-antibody-50-ug' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3020', 'antibody_id' => '552', 'name' => 'Pseudouridine Antibody (sample size)', 'description' => '', 'label1' => 'Validation data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200247-10', 'old_catalog_number' => '', 'sf_code' => 'C15200247-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '0', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'pseudouridine-monoclonal-antibody-10-ug', 'meta_title' => 'Pseudouridine Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Pseudouridine (psU) Monoclonal Antibody validated in RIP. 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Batch-specific data available on the website. Sample size available', 'modified' => '2022-01-05 16:17:23', 'created' => '2019-03-19 15:29:12', 'ProductsGroup' => array( 'id' => '296', 'product_id' => '3020', 'group_id' => '261' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '5017', 'product_id' => '3019', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'eng' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '1046', 'name' => 'Pseudouridine Monoclonal Antibody datasheet', 'description' => '<p>Monoclonal antibody raised in mouse against pseudouridine (psU) conjugated to BSA.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/pseudouridine-monoclonal-antibody-50-ug.pdf', 'slug' => 'pseudouridine-monoclonal-antibody-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-03-19 15:40:27', 'created' => '2019-03-19 15:40:27', 'ProductsDocument' => array( 'id' => '2747', 'product_id' => '3019', 'document_id' => '1046' ) ) $sds = array( 'id' => '1581', 'name' => 'Pseudouridine antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Pseudouridine/SDS-C15200247-Pseudouridine_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-30 16:57:10', 'created' => '2021-08-30 16:57:10', 'ProductsSafetySheet' => array( 'id' => '2752', 'product_id' => '3019', 'safety_sheet_id' => '1581' ) ) $publication = array( 'id' => '4988', 'name' => 'Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis', 'authors' => 'Xi Wang et al.', 'description' => '<h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Background</h3> <p>Previous studies have indicated that ψ-modified small RNAs play crucial roles in tumor metastasis. However, the ψ-modified small RNAs during metastasis of PTC are still unclear.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Methods</h3> <p>We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Results</h3> <p>Our results demonstrated that PUS7 was down-regulated in PTC and was closely associated with metastasis. Moreover, the ψ modification of pre-miR-8082 was found to be decreased, resulting in down-expression of pre-miR-8082 and miR-8082, leading to the loss of the inhibitory effect on CD47, thereby promoting tumor migration.</p> <h3 class="c-article__sub-heading" data-test="abstract-sub-heading">Conclusions</h3> <p>Our study demonstrates that PUS7 promotes the inhibition of CD47 and inhibits metastasis of PTC cells by regulating the ψ modification of pre-miR-8082. These results suggest that PUS7 and ψ pre-miR-8082 may serve as potential targets and diagnostic markers for PTC metastasis.</p>', 'date' => '2024-10-14', 'pmid' => 'https://link.springer.com/article/10.1186/s12935-024-03482-3', 'doi' => 'https://doi.org/10.1186/s12935-024-03482-3', 'modified' => '2024-10-18 11:41:05', 'created' => '2024-10-18 11:41:05', 'ProductsPublication' => array( 'id' => '7592', 'product_id' => '3019', 'publication_id' => '4988' ) ) $externalLink = ' <a href="https://link.springer.com/article/10.1186/s12935-024-03482-3" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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