RbAp 46/48 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15100033
(AC-033-100)

100 µl

$260.00
Bulk order

Alternative names: RBBP7/4, RBBP-7/4, RBAP46/48, NURF 55

Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastomabinding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.

Lot	001
Concentration	not determined
Species reactivity	Human
Type	Monoclonal
Purity	Ascites fluid
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:1,000	Fig 1

Validation Data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48
Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# AC-033-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.

Documents
Datasheet hRbAp46-48 AC-033-100 DATASHEET
Datasheet description Download

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><span>Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastomabinding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.</span></p>',
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15100033_fig1.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="200" width="77" /></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# AC-033-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.</small></p>
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			'description' => 'RbAp48 (UniProtKB/Swiss-Prot entry Q09028) is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. These include (1) the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; (2) the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; (3) the nucleosome remodelling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; (4) the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and (5) the NURF (nucleosome remodeling factor) complex.',
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<th>References</th>
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<td>Fig 1</td>
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<p><span>Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastomabinding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.</span></p>',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# AC-033-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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