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Other names: RBBP-5, RBQ3, SWD1
Polyclonal antibody raised in rabbit against human RBBP5 (RB Binding Protein 5), using a synthetic peptide containing a sequence from the C-terminus of the protein1.
| Lot | A300-109A2 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal, ChIP grade, ChIP-seq grade |
| Purity | Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. |
| Host | Rabbit |
| Storage Conditions | Store at 4°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 2 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:1,000 | Fig 3 |
| IP | 10 μg per IP | Fig 4 |
| IHC | 1:1,000 | Fig 5 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
1Manufactured by Bethyl Laboratories, Inc., Texas, USA
Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5
ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).
Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5
Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).
Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5
FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.
RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| IHC Immunohistochemistry Read more |
| RBBP5 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human RBBP5 (RB Binding Protein 5), using a syntheti... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| SDS C15410342 RBBP5 Antibody US en | Download |
| SDS C15410342 RBBP5 Antibody GB en | Download |
| SDS C15410342 RBBP5 Antibody BE fr | Download |
| SDS C15410342 RBBP5 Antibody ES es | Download |
| SDS C15410342 RBBP5 Antibody DE de | Download |
| SDS C15410342 RBBP5 Antibody JP ja | Download |
| SDS C15410342 RBBP5 Antibody BE nl | Download |
| SDS C15410342 RBBP5 Antibody FR fr | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: RBBP5 Antibody (Diagenode Cat# C15410342 Lot# A300-109A2). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. 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Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.' $meta_title = 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode' $product = array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. 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Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ) ), 'Group' => array( 'Group' => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ), 'Master' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '986', 'name' => 'RBBP5 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human RBBP5 (RB Binding Protein 5), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_RBBP5_C15410342.pdf', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 11:18:45', 'created' => '2018-01-03 11:18:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3772', 'name' => 'SDS C15410342 RBBP5 Antibody US en', 'language' => 'en', 'url' => 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '516', 'name' => 'RBBP5 polyclonal antibody', 'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A300-109A2', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. 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Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.' $meta_title = 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode' $product = array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '516', 'name' => 'RBBP5 polyclonal antibody', 'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A300-109A2', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ) ), 'Group' => array( 'Group' => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ), 'Master' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. 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It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '516', 'name' => 'RBBP5 polyclonal antibody', 'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A300-109A2', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/rbbp5-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.' $meta_title = 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode' $product = array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '516', 'name' => 'RBBP5 polyclonal antibody', 'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A300-109A2', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ) ), 'Group' => array( 'Group' => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ), 'Master' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. 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Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '516', 'name' => 'RBBP5 polyclonal antibody', 'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A300-109A2', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/rbbp5-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.' $meta_title = 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode' $product = array( 'Product' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410342', 'old_catalog_number' => '', 'sf_code' => 'C15410342-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 25, 2017', 'slug' => 'rbbp5-polyclonal-antibody-100-ul', 'meta_title' => 'RBBP5 Antibody - ChIP-seq Grade (C15410342) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'RBBP5 (RB Binding Protein 5) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IHC. Batch-specific data available on the website. Other names: RBBP-5, RBQ3, SWD1.', 'modified' => '2024-01-17 19:15:14', 'created' => '2017-12-21 10:52:54', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '516', 'name' => 'RBBP5 polyclonal antibody', 'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A300-109A2', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>10 μg per IP</td> <td>Fig 4</td> </tr> <tr> <td>IHC</td> <td>1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'rbbp5-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-30 14:25:11', 'created' => '2017-12-21 10:47:00', 'select_label' => '516 - RBBP5 polyclonal antibody (A300-109A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ) ), 'Group' => array( 'Group' => array( 'id' => '343', 'name' => 'C15410342', 'product_id' => '2921', 'modified' => '2020-12-30 14:30:41', 'created' => '2020-12-30 14:30:41' ), 'Master' => array( 'id' => '2921', 'antibody_id' => '516', 'name' => 'RBBP5 Antibody', 'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p> <p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '3086', 'product_id' => '2921', 'document_id' => '38' ) ) $sds = array( 'id' => '3777', 'name' => 'SDS C15410342 RBBP5 Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/RBBP5/SDS-C15410342-RBBP5_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-17 19:14:45', 'created' => '2024-01-17 19:14:45', 'ProductsSafetySheet' => array( 'id' => '6173', 'product_id' => '2921', 'safety_sheet_id' => '3777' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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