RFX5 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15410326

100 μg/100 μl

$440.00
Bulk order

Polyclonal antibody raised in rabbit against RFX5 (Regulatory Factor X 5), using a KLH conjugated synthetic peptide from the central part of the protein.

Lot	001
Concentration	1 μg/μl
Species reactivity	Human
Type	Polyclonal
Purity	Purified by ion exchange chromatography
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:2,500	Fig 1

Validation data

Figure 1. Western blot analysis using the Diagenode antibody directed against RFX5
Nuclear extracts from Raji B cells (35 μg) were analysed by Western blot using the Diagenode antibody against RFX (Cat. No. C15410326) diluted 2,500. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Target description
RFX5 (UniProt/Swiss-Prot entry P48382) binds to the X box of MHC-II promoters thereby activating transcription, and mediates the cooperative binding between RFX and NFY. Deficiency of RFX5 leads to a lack of MHC-II expression which is characterized by a severe immunodeficiency syndrome called MHC-II deficiency.

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against RFX5</strong><br />Nuclear extracts from Raji B cells (35 &mu;g) were analysed by Western blot using the Diagenode antibody against RFX (Cat. No. C15410326) diluted 2,500. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
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<th>Suggested dilution</th>
<th>References</th>
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<td>Western Blotting</td>
<td>1:2,500</td>
<td>Fig 1</td>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against RFX5</strong><br />Nuclear extracts from Raji B cells (35 &mu;g) were analysed by Western blot using the Diagenode antibody against RFX (Cat. No. C15410326) diluted 2,500. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against RFX5</strong><br />Nuclear extracts from Raji B cells (35 &mu;g) were analysed by Western blot using the Diagenode antibody against RFX (Cat. No. C15410326) diluted 2,500. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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	'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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RFX5 polyclonal antibody - C15410326 DATASHEET Polyclonal antibody raised in rabbit against RFX5 (Regulatory Factor X 5), using a KLH conjugated...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

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