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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
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<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
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<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
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<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<td>Western Blotting</td>
<td>1:4,000</td>
<td>Fig 1</td>
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<tr>
<td>Immunoprecipitation</td>
<td>5 μg/IP</td>
<td>Fig 2</td>
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<td>Immunofluorescence</td>
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<td>Fig 3</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="CRISPR/Cas9 Antibody validated in WB " caption="false" width="284" height="289" /></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-IF.jpg" alt="CRISPR/Cas9 Antibody validated in IF" caption="false" width="284" height="91" /></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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'name' => '<i>S. aureus</i> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
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<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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'description' => '<p>Previous studies from others and us have demonstrated that CRISPR genome editing could offer a promising therapeutic strategy to restore dystrophin expression and function in the skeletal muscle and heart of Duchenne muscular dystrophy (DMD) mouse models. However, the long-term efficacy and safety of CRISPR genome-editing therapy for DMD has not been well established. We packaged both SaCas9 and guide RNA (gRNA) together into one AAVrh.74 vector, injected two such vectors (targeting intron 20 and intron 23, respectively) into mdx pups at day 3 and evaluated the mice at 19 months. We found that AAVrh.74-mediated life-long CRISPR genome editing in mdx mice restored dystrophin expression and improved cardiac function without inducing serious adverse effects. PCR analysis and targeted deep sequencing showed that the DSBs were mainly repaired by the precise ligation of the two cut sites. Serological and histological examination of major vital organs did not reveal any signs of tumor development or other deleterious defects arising from CRISPR genome editing. These results support that in vivo CRISPR genome editing could be developed as a safe therapeutic treatment for DMD and potentially other diseases.</p>',
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
<ul>
<li>Excellent results in <strong>WB</strong>,<strong> IP</strong> and<strong> IF</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200230-WB.jpg" alt="WB figure 1" /></p>
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<div class="small-8 columns">
<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
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<div class="small-7 columns">
<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
</ul>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
</tbody>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against S. aureus CRISPR/ Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus CRISPR/Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2, 4 and 6) using 5 μg of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230). The immunoprecipitated proteins were subsequently analysed by Western blot. The results obtained with the Cas9 antibody are shown in lane 3 and 4. The negative control (IP with beads only) is shown in lane 5 and 6, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against S. aureus CRISPR/Cas9</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/Cas9 antibody (cat. No. C15200230) diluted 1:400 in blocking solution at 4°C o/n, followed by incubation with an anti-mouse secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<h6 style="height:60px">S. aureus CRISPR/Cas9 Antibody (N-terminal)</h6>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-WB.jpg" alt="WB figure 1" /></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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