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Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.
| Lot | 41906 |
|---|---|
| Concentration | 1.32 μg/μl |
| Species reactivity | Human, mouse, rat: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP* | 5 µg/ChIP | Fig 1 |
| Western blotting | 1:500 - 1:1,000 | Fig 2, 3 |
| Immunoprecipitation | 5 µg/IP | Fig 4 |
| IF | 1:500 | Fig 5 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3
ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.
Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3
Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3
Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3
Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.
Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33
HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.
SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
| Datasheet SMYD3 C15410253 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: SMYD3 Antibody (sample size) (Diagenode Cat# C15410253-25 Lot# 41906). Click here to copy to clipboard. Using our products in your publication? Let us know! |
SMYD3: a new regulator of the early steps of adipocyte differentiation |
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' $meta_title = 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode' $product = array( 'Product' => array( 'id' => '2359', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody (sample size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410253-25', 'old_catalog_number' => '', 'sf_code' => 'C15410253-32380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '130', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '20365', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smyd3-polyclonal-antibody-classic-25-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, IHC, IP and IF. 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Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP*</td> <td>5 µg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:500 - 1:1,000</td> <td>Fig 2, 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>5 µg/IP</td> <td>Fig 4</td> </tr> <tr> <td>IF</td> <td>1:500</td> <td>Fig 5</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-04 15:39:16', 'created' => '2015-07-28 12:39:00', 'select_label' => '424 - SMYD3 polyclonal antibody (41906 - 1.32 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '92', 'name' => 'C15410253', 'product_id' => '2360', 'modified' => '2016-02-18 22:03:07', 'created' => '2016-02-18 22:03:07' ), 'Master' => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. 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Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '605', 'name' => 'Datasheet SMYD3 C15410253', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMYD3_C15410253.pdf', 'slug' => 'datasheet-smyd3-c15410253', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/smyd3-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '2359', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody (sample size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410253-25', 'old_catalog_number' => '', 'sf_code' => 'C15410253-32380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '130', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '20365', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smyd3-polyclonal-antibody-classic-25-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, IHC, IP and IF. ', 'modified' => '2022-01-05 15:05:11', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '99', 'product_id' => '2359', 'group_id' => '92' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4164', 'product_id' => '2359', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1951', 'product_id' => '2359', 'document_id' => '38' ) ) $publication = array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( 'id' => '7055', 'product_id' => '2359', 'publication_id' => '4839' ) ) $externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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' $meta_title = 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode' $product = array( 'Product' => array( 'id' => '2359', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody (sample size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. 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The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-04 15:39:16', 'created' => '2015-07-28 12:39:00', 'select_label' => '424 - SMYD3 polyclonal antibody (41906 - 1.32 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '92', 'name' => 'C15410253', 'product_id' => '2360', 'modified' => '2016-02-18 22:03:07', 'created' => '2016-02-18 22:03:07' ), 'Master' => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '605', 'name' => 'Datasheet SMYD3 C15410253', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMYD3_C15410253.pdf', 'slug' => 'datasheet-smyd3-c15410253', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/smyd3-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. 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', 'modified' => '2022-01-05 15:05:11', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '99', 'product_id' => '2359', 'group_id' => '92' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4164', 'product_id' => '2359', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1951', 'product_id' => '2359', 'document_id' => '38' ) ) $publication = array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( 'id' => '7055', 'product_id' => '2359', 'publication_id' => '4839' ) ) $externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP*</td> <td>5 µg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:500 - 1:1,000</td> <td>Fig 2, 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>5 µg/IP</td> <td>Fig 4</td> </tr> <tr> <td>IF</td> <td>1:500</td> <td>Fig 5</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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' $meta_title = 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode' $product = array( 'Product' => array( 'id' => '2359', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody (sample size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410253-25', 'old_catalog_number' => '', 'sf_code' => 'C15410253-32380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '130', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '20365', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smyd3-polyclonal-antibody-classic-25-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, IHC, IP and IF. ', 'modified' => '2022-01-05 15:05:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '424', 'name' => 'SMYD3 polyclonal antibody', 'description' => 'SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.', 'clonality' => '', 'isotype' => '', 'lot' => '41906', 'concentration' => '1.32 μg/μl', 'reactivity' => 'Human, mouse, rat: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP*</td> <td>5 µg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:500 - 1:1,000</td> <td>Fig 2, 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>5 µg/IP</td> <td>Fig 4</td> </tr> <tr> <td>IF</td> <td>1:500</td> <td>Fig 5</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-04 15:39:16', 'created' => '2015-07-28 12:39:00', 'select_label' => '424 - SMYD3 polyclonal antibody (41906 - 1.32 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '92', 'name' => 'C15410253', 'product_id' => '2360', 'modified' => '2016-02-18 22:03:07', 'created' => '2016-02-18 22:03:07' ), 'Master' => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '605', 'name' => 'Datasheet SMYD3 C15410253', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMYD3_C15410253.pdf', 'slug' => 'datasheet-smyd3-c15410253', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/smyd3-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. 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', 'modified' => '2022-01-05 15:05:11', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '99', 'product_id' => '2359', 'group_id' => '92' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. 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The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( 'id' => '7055', 'product_id' => '2359', 'publication_id' => '4839' ) ) $externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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' $meta_title = 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode' $product = array( 'Product' => array( 'id' => '2359', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody (sample size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410253-25', 'old_catalog_number' => '', 'sf_code' => 'C15410253-32380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '130', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '20365', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smyd3-polyclonal-antibody-classic-25-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, IHC, IP and IF. 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Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP*</td> <td>5 µg/ChIP</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:500 - 1:1,000</td> <td>Fig 2, 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>5 µg/IP</td> <td>Fig 4</td> </tr> <tr> <td>IF</td> <td>1:500</td> <td>Fig 5</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-05-04 15:39:16', 'created' => '2015-07-28 12:39:00', 'select_label' => '424 - SMYD3 polyclonal antibody (41906 - 1.32 μg/μl - Human, mouse, rat: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '92', 'name' => 'C15410253', 'product_id' => '2360', 'modified' => '2016-02-18 22:03:07', 'created' => '2016-02-18 22:03:07' ), 'Master' => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. 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Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '605', 'name' => 'Datasheet SMYD3 C15410253', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMYD3_C15410253.pdf', 'slug' => 'datasheet-smyd3-c15410253', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/smyd3-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '2359', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody (sample size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410253-25', 'old_catalog_number' => '', 'sf_code' => 'C15410253-32380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '130', 'price_USD' => '160', 'price_GBP' => '125', 'price_JPY' => '20365', 'price_CNY' => '', 'price_AUD' => '400', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smyd3-polyclonal-antibody-classic-25-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, IHC, IP and IF. ', 'modified' => '2022-01-05 15:05:11', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '99', 'product_id' => '2359', 'group_id' => '92' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2360', 'antibody_id' => '424', 'name' => 'SMYD3 Antibody', 'description' => '<p><strong>Other names:</strong> ZMYND1, ZNFN3A1, KMT3E, BA74P14.1</p> <p>Polyclonal antibody raised in rabbit against <strong>SMYD3 (SET and MYND domain containing 3)</strong>, using a recombinant protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-CHIP.jpg" alt="SMYD3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3</strong><br /> ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-WB.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410253-wb-2.jpg" alt="SMYD3 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3</strong><br /> Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IP.jpg" alt="SMYD3 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410253-IF.jpg" alt="SMYD3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33</strong><br /> HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410253-100', 'old_catalog_number' => '', 'sf_code' => 'C15410253-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 9, 2017', 'slug' => 'smyd3-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMYD3 Antibody - ChIP Grade (C15410253) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'SMYD3 (SET and MYND domain containing 3) Polyclonal Antibody validated in ChIP-qPCR, ELISA, IHC, IP, WB and IF. Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1', 'modified' => '2022-01-05 15:04:59', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4164', 'product_id' => '2359', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1951', 'product_id' => '2359', 'document_id' => '38' ) ) $publication = array( 'id' => '4839', 'name' => 'SMYD3: a new regulator of the early steps of adipocyte differentiation', 'authors' => 'Gilardi F. et al.', 'description' => '<p>In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tissue expansion, as opposed to the development of inflammation and detrimental metabolic consequences. However, the molecular mechanisms underlying this fine-tuned regulation are far from being understood. Methods We analyzed by mass spectrometry-based proteomics visceral white adipose tissue (vWAT) samples collected from C57BL6 mice fed with a HFD for 8 weeks. A subset of these mice, called low inflammation (Low-INFL), showed a low susceptibility to the onset of adipose tissue inflammation, as opposed to those developing the expected inflammatory response (Hi-INFL). We identified the discriminants between Low-INFL and Hi-INFL vWAT samples and explored their function in Adipose Derived human Mesenchymal Stem Cells (AD-hMSCs) differentiated to adipocytes. Results We quantified 6051 proteins. Among the candidates that most differentiate Low-INFL from Hi-INFL vWAT, we found proteins involved in adipocyte function, including adiponectin and hormone sensitive lipase, suggesting that adipocyte differentiation is enhanced in Low-INFL, as compared to Hi-INFL. The chromatin modifier SET and MYND Domain Containing 3 (SMYD3), whose function in adipose tissue was so far unknown, was another top-scored hit. SMYD3 expression was significantly higher in Low-INFL vWAT, as confirmed by western blot analysis. In vitro, we found that SMYD3 mRNA and protein levels decrease rapidly along the differentiation process of AD-hMSCs. Moreover, SMYD3 knock-down at the beginning of adipocyte differentiation resulted in reduced cell proliferation and, at longer term, reduced lipid accumulation in adipocytes. Conclusions Our study describes an important role for SMYD3 as a newly discovered regulator of adipocyte proliferation during the early steps of adipogenesis.</p>', 'date' => '2023-06-01', 'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1', 'doi' => '10.21203/rs.3.rs-3126142/v1', 'modified' => '2023-08-01 14:00:06', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( 'id' => '7055', 'product_id' => '2359', 'publication_id' => '4839' ) ) $externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-3126142%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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