Alternative names: STAT113, P113, ISGF-3
Monoclonal antibody raised in mouse against STAT2, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.0 μg/μl |
| Species reactivity | Human, mouse |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ELISA | 1:5,000 - 1:10,000 | |
| Western Blotting | 1:1,000 | Fig 1 |
| Immunohistochemistry | 1:2.5 μg/mL | Fig 2 |
* Optimal dilutions/concentrations should be determined by the researcher.
Figure 1. STAT2 western blot results
Western blot was performed on 1 μg HeLa whole cell lysate with the STAT2 monoclonal antibody (Cat. No. C15200210). The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with the Diagenode STAT2 antibody diluted 1:1,000 in 3% BSA/TBST overnight at 4C.
Figure 2. STAT2 antibody immunohistochemistry results
The Diagenode STAT2 monoclonal antibody was used at a 2.5 μg/mL to detect STAT2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (figure 2). Expression of STAT2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
 How to properly cite our product/service in your workWe strongly recommend using this: STAT2 Antibody (Hologic Diagenode Cat# C15200210 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
