Polyclonal antibody raised in rabbit against TAF1 (Transcription Initiation Factor TFIID Subunit 1), using a KLH-conjugated synthetic peptide.
| Lot | 002 |
|---|---|
| Concentration | 1.1 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:10,000 - 1:50,000 | |
| Western Blotting | 1:100 - 1:2,000 | Fig 1 |
| Immunochemistry | 10 μg/ml | Fig 2 |
Figure 1. TAF1 antibody Western blot results
Western blot using the Diagenode TAF1 antibody to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3) and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1.
Figure 2. TAF1 antibody Immunohistochemistry results
Immunohistochemistry of TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: TAF1 at 10 μg/mL for 1h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 dilution for 45 min at RT. Staining: TAF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
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