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Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.0 μg/μl |
| Species reactivity | |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:10,000 - 1:60,000 | |
| Immunohistochemistry | 1:500 - 1:3,000 | |
| Western Blotting | 1:5,000 - 1:10,000 | Fig 3 |
Figure 1. V5 Epitope tag antibody Western blot results
The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS- PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| IHC Immunohistochemistry Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet V5 Epitope tag C15410270 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: V5 Epitope tag Antibody (Diagenode Cat# C15410270 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Release of Immunomodulatory Ebola Virus Glycoprotein-Containing Microvesicles Is Suppressed by Tetherin in a Species-Specific Manner. |
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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. ', 'meta_title' => 'V5 Epitope tag Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2389', 'antibody_id' => '435', 'name' => 'V5 Epitope tag Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410270-WB.jpg" alt="Western blot results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. V5 Epitope tag antibody Western blot results</strong><br /> The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. 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After blocking the membrane was probed with the primary antibody overnight at 4° C. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410270', 'old_catalog_number' => '', 'sf_code' => 'C15410270-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'v5-epitope-tag-polyclonal-antibody-classic-100-mg', 'meta_title' => 'V5 Epitope tag Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. 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However, determinants of GP-virosome release and their functionality are poorly understood. We characterized GP-mediated virosome formation and delineated the role of the antiviral factor tetherin (BST2, CD317) in this process. Residues in the EBOV-GP receptor-binding domain (RBD) promote GP-virosome secretion, while tetherin suppresses GP-virosomes by interactions involving the GP-transmembrane domain. Tetherin from multiple species interfered with GP-virosome release, and tetherin from the natural fruit bat reservoir showed the highest inhibitory activity. Moreover, analyses of GP from various ebolavirus strains, including the EBOV responsible for the West African epidemic, revealed the most efficient GP-virosome formation by highly pathogenic ebolaviruses. Finally, EBOV-GP-virosomes were immunomodulatory and acted as decoys for EBOV-neutralizing antibodies. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. ', 'meta_title' => 'V5 Epitope tag Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2389', 'antibody_id' => '435', 'name' => 'V5 Epitope tag Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410270-WB.jpg" alt="Western blot results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. V5 Epitope tag antibody Western blot results</strong><br /> The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS- PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410270', 'old_catalog_number' => '', 'sf_code' => 'C15410270-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'v5-epitope-tag-polyclonal-antibody-classic-100-mg', 'meta_title' => 'V5 Epitope tag Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-12 14:54:29', 'created' => '2015-07-28 15:05:06', 'select_label' => '435 - V5 Epitope tag polyclonal antibody - Classic (001 - 1.0 μg/μl - - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. ' $meta_title = 'V5 Epitope tag Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2389', 'antibody_id' => '435', 'name' => 'V5 Epitope tag Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410270-WB.jpg" alt="Western blot results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. V5 Epitope tag antibody Western blot results</strong><br /> The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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However, determinants of GP-virosome release and their functionality are poorly understood. We characterized GP-mediated virosome formation and delineated the role of the antiviral factor tetherin (BST2, CD317) in this process. Residues in the EBOV-GP receptor-binding domain (RBD) promote GP-virosome secretion, while tetherin suppresses GP-virosomes by interactions involving the GP-transmembrane domain. Tetherin from multiple species interfered with GP-virosome release, and tetherin from the natural fruit bat reservoir showed the highest inhibitory activity. Moreover, analyses of GP from various ebolavirus strains, including the EBOV responsible for the West African epidemic, revealed the most efficient GP-virosome formation by highly pathogenic ebolaviruses. Finally, EBOV-GP-virosomes were immunomodulatory and acted as decoys for EBOV-neutralizing antibodies. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'eng' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>' $name = 'WB' $document = array( 'id' => '558', 'name' => 'Datasheet V5 Epitope tag C15410270', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_V5_Epitope_tag_C15410270.pdf', 'slug' => 'datasheet-v5-epitope-tag-c15410270', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '990', 'product_id' => '2389', 'document_id' => '558' ) ) $publication = array( 'id' => '3679', 'name' => 'Release of Immunomodulatory Ebola Virus Glycoprotein-Containing Microvesicles Is Suppressed by Tetherin in a Species-Specific Manner.', 'authors' => 'Nehls J, Businger R, Hoffmann M, Brinkmann C, Fehrenbacher B, Schaller M, Maurer B, Schönfeld C, Kramer D, Hailfinger S, Pöhlmann S, Schindler M', 'description' => '<p>The Ebola virus glycoprotein (EBOV-GP) forms GP-containing microvesicles, so-called virosomes, which are secreted from GP-expressing cells. However, determinants of GP-virosome release and their functionality are poorly understood. We characterized GP-mediated virosome formation and delineated the role of the antiviral factor tetherin (BST2, CD317) in this process. Residues in the EBOV-GP receptor-binding domain (RBD) promote GP-virosome secretion, while tetherin suppresses GP-virosomes by interactions involving the GP-transmembrane domain. Tetherin from multiple species interfered with GP-virosome release, and tetherin from the natural fruit bat reservoir showed the highest inhibitory activity. Moreover, analyses of GP from various ebolavirus strains, including the EBOV responsible for the West African epidemic, revealed the most efficient GP-virosome formation by highly pathogenic ebolaviruses. Finally, EBOV-GP-virosomes were immunomodulatory and acted as decoys for EBOV-neutralizing antibodies. Our results indicate that GP-virosome formation might be a determinant of EBOV immune evasion and pathogenicity, which is suppressed by tetherin.</p>', 'date' => '2019-02-12', 'pmid' => 'http://www.pubmed.gov/30759394', 'doi' => '10.1016/j.celrep.2019.01.065', 'modified' => '2019-07-01 11:19:19', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( 'id' => '3771', 'product_id' => '2389', 'publication_id' => '3679' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30759394" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. ', 'meta_title' => 'V5 Epitope tag Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2389', 'antibody_id' => '435', 'name' => 'V5 Epitope tag Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410270-WB.jpg" alt="Western blot results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. V5 Epitope tag antibody Western blot results</strong><br /> The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS- PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410270', 'old_catalog_number' => '', 'sf_code' => 'C15410270-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'v5-epitope-tag-polyclonal-antibody-classic-100-mg', 'meta_title' => 'V5 Epitope tag Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'V5 Epitope tag Polyclonal Antibody validated in ELISA, IHC and WB. ', 'modified' => '2022-01-05 16:06:56', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '435', 'name' => 'V5 Epitope tag polyclonal antibody - Classic', 'description' => 'This antibody recognizes the V5 epitope tag. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.0 μg/μl', 'reactivity' => '', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:10,000 - 1:60,000</td> <td></td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:500 - 1:3,000</td> <td></td> </tr> <tr> <td>Western Blotting</td> <td>1:5,000 - 1:10,000</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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' $meta_title = 'V5 Epitope tag Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2389', 'antibody_id' => '435', 'name' => 'V5 Epitope tag Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410270-WB.jpg" alt="Western blot results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. V5 Epitope tag antibody Western blot results</strong><br /> The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS- PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. 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', 'modified' => '2022-01-05 16:06:56', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '435', 'name' => 'V5 Epitope tag polyclonal antibody - Classic', 'description' => 'This antibody recognizes the V5 epitope tag. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.0 μg/μl', 'reactivity' => '', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:10,000 - 1:60,000</td> <td></td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:500 - 1:3,000</td> <td></td> </tr> <tr> <td>Western Blotting</td> <td>1:5,000 - 1:10,000</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'eng' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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