Alternative name: KAISO-L1
Polyclonal antibody raised in rabbit against human ZBTB4 (zinc finger and BTB domain containing 4), using four different KLH-conjugated synthetic peptides.
| Lot | A180-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:1,000 - 1:5,000 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ZBTB4 (Cat. No. CS-142-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:50,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against ZBTB4
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ZBTB4 (Cat. No. CS-142-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 105 kDa); the marker (in kDa) is shown on the left.
 How to properly cite our product/service in your workWe strongly recommend using this: ZBTB4 Antibody (Hologic Diagenode Cat# C15310142 Lot# A180-001 ). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
