Diagenode

Low affinity CTCF binding drives transcriptional regulation whereashigh affinity binding encompasses architectural functions


Marina-Zárate E. et al.

CTCF is a DNA-binding protein which plays critical roles in chromatin structure organization and transcriptional regulation; however, little is known about the functional determinants of different CTCF-binding sites (CBS). Using a conditional mouse model, we have identified one set of CBSs that are lost upon CTCF depletion (lost CBSs) and another set that persists (retained CBSs). Retained CBSs are more similar to the consensus CTCF-binding sequence and usually span tandem CTCF peaks. Lost CBSs are enriched at enhancers and promoters and associate with active chromatin marks and higher transcriptional activity. In contrast, retained CBSs are enriched at TAD and loop boundaries. Integration of ChIP-seq and RNA-seq data has revealed that retained CBSs are located at the boundaries between distinct chromatin states, acting as chromatin barriers. Our results provide evidence that transient, lost CBSs are involved in transcriptional regulation, whereas retained CBSs are critical for establishing higher-order chromatin architecture.

Tags
Antibody
iDeal ChIP-seq Kit for Transcription Factors

Share this article

Published
February, 2023

Source

Products used in this publication

  • ChIP kit icon
    C01010055
    iDeal ChIP-seq kit for Transcription Factors
  • cut and tag antibody icon
    C15410210-50
    CTCF Antibody

Events

 See all events

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy