Diagenode

Steroid receptor-assisted loading modulates transcriptional responses in prostate cancer cells


Johannes Hiltunen et al.

Steroid receptors are involved in a wide array of crosstalk mechanisms that regulate diverse biological processes, with significant implications in diseases, particularly in cancers. In prostate cancer, indirect crosstalk between androgen receptor (AR) and glucocorticoid receptor (GR) is well-documented, where GR replaces antiandrogen-inactivated AR becoming the disease driver. However, the existence and impact of direct chromatin crosstalk between AR and GR in prostate cancer have remained elusive. Our genome-wide investigations reveal that AR activation significantly expands GR chromatin binding. Mechanistically, AR induces remodeling of closed chromatin sites, facilitating GR binding to inaccessible sites. Importantly, coactivation of AR and GR results in distinct transcriptional responses at both the cell population and single-cell levels. Intriguingly, pathways affected by these transcriptional changes are generally associated with improved patient survival. Thus, the direct crosstalk between AR and GR yields markedly different outcomes from the known role of GR in circumventing AR blockade by antiandrogens.

Tags
Tagmentase

Share this article

Published
November, 2024

Source

Products used in this publication

  • Tubes
    C01070012-30
    Tagmentase (Tn5 transposase) - loaded

Events

  • Pacbio PRISM
    Danang, Vietnam
    Apr 14-Apr 16, 2025
  • American Association of Cancer Research (AACR)
    Chicago (IL), USA
    Apr 25-Apr 30, 2025
 See all events

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy