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3-methylcytosine (3-mC) polyclonal antibody

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Catalog Number

Format

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C15410209

50 μg/28 μl

$260.00
Bulk order

Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.

Lot	A2111-0010
Concentration	1.80 µg/µl
Species reactivity	Human, mouse, other (wide range)
Type	Polyclonal
Purity	Protein G purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Dot Blotting	1:400	Fig 1

^* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.

Validation Data

Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC
To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.
Target description
3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.
実験手法
DB
Dot blotting Read more

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<td>Fig 1</td>
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<p><em>Looking for hMeDIP-seq protocol? <a href="https://go.diagenode.com/l/928883/2022-01-07/2m1ht" target="_blank" title="Contact us">Contact us</a></em></p>
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<li>hmeDNA and unmethylated DNA sequences and primer pairs</li>
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<p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p>
<h3><span>Features</span></h3>
<ul>
<li>Starting DNA amount: <strong>10 ng &ndash; 1 &micro;g</strong></li>
<li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li>
<li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li>
<li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li>
<li><strong>High reproducibility</strong> between replicates and repetitive experiments</li>
<li>Compatible with <strong>all species&nbsp;</strong></li>
</ul>
<p>&nbsp;</p>
<div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div>
<div class="small-12 medium-8 large-8 columns">
<h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3>
<h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3>
</div>
<p></p>
<p></p>
<p></p>
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<h3></h3>',
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			'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p>
<h3><span>How it works</span></h3>
<p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP&rsquo;d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p>
<center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center>
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			'label2' => 'MeDIP-qPCR',
			'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode&rsquo;s IP-Star Compact Automated System (please refer to the kit manual).</p>
<ul>
<li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li>
<li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li>
<li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li>
<li><strong>Highly validated protocol</strong></li>
<li>Automated protocol supplied</li>
</ul>
<center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center>
<p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p>
<p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 &micro;g or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p>
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			'label3' => 'MeDIP-seq',
			'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p>
<ul style="list-style-type: circle;">
<li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 &mu;g). It can be a home-made solution or a commercial one.</li>
<li>Choose the indexing system that fits your needs considering the following features:</li>
<ul>
<ul>
<ul>
<li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li>
<li>Number of barcodes</li>
<li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li>
<li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li>
</ul>
</ul>
</ul>
<li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li>
</ul>
<p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p>
<ul style="list-style-type: circle;">
<li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li>
</ul>
<ul style="list-style-type: circle;">
<li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li>
</ul>
<h3><span>MeDIP-seq workflow</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center>
<h3><span>Example of results</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1&mu;g &ndash; 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency &gt;98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 &mu;g (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p>
<p></p>
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			'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
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<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
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<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
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<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
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<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
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			'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p>
<p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p>
<p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p>
<p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p>
<p>Check out the list below to see all proposed antibodies for DNA modifications.</p>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody US en',
			'language' => 'en',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2024-01-17 17:09:15',
			'created' => '2024-01-17 17:09:15',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '3636',
			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-01-17 17:08:39',
			'created' => '2024-01-17 17:08:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3635',
			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody DE de',
			'language' => 'de',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-01-17 17:08:22',
			'created' => '2024-01-17 17:08:22',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3639',
			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-01-17 17:09:32',
			'created' => '2024-01-17 17:09:32',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3634',
			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody ES es',
			'language' => 'es',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-01-17 17:08:05',
			'created' => '2024-01-17 17:08:05',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3637',
			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-01-17 17:08:58',
			'created' => '2024-01-17 17:08:58',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3641',
			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-01-17 17:10:07',
			'created' => '2024-01-17 17:10:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
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	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
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$other_formats = array()
$edit = ''
$testimonials = ''
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                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
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                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
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                    '690',
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        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010031</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
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<form action="/jp/carts/add/1882" id="CartAdd/1882Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1882" id="CartProductId"/>
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>hMeDIP kit x16 (monoclonal mouse antibody)個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010031',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010031',
                    '690',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1882" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">hMeDIP kit x16 (monoclonal mouse antibody)</h6>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/magmedip-kit-x48-48-rxns"><img src="/img/product/kits/C02010021-magmedip-qpcr.jpg" alt="MagMeDIP qPCR Kit box" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010021</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1880" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1880" id="CartAdd/1880Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1880" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>MagMeDIP Kit個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('MagMeDIP Kit',
                    'C02010021',
                     '750',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
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                    'C02010021',
                    '750',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="magmedip-kit-x48-48-rxns" data-reveal-id="cartModal-1880" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MagMeDIP qPCR kit</h6>
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    </div>
</li>
<li>
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        <div class="small-12 columns">
                         <a href="/jp/p/methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020010</span>
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            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MethylCap kit</h6>
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    </div>
</li>
<li>
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                         <a href="/jp/p/premium-bisulfite-kit-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030030</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
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<form action="/jp/carts/add/1892" id="CartAdd/1892Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1892" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Premium Bisulfite kit個カートに追加。</p>

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            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                     '240',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                    '240',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-bisulfite-kit-50-rxns" data-reveal-id="cartModal-1892" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
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'
$related = array(
	'id' => '1892',
	'antibody_id' => null,
	'name' => 'Premium Bisulfite kit',
	'description' => '<p style="text-align: center;"><a href="https://www.diagenode.com/files/products/kits/Premium_Bisulfite_kit_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '50 rxns',
	'catalog_number' => 'C02030030',
	'old_catalog_number' => '',
	'sf_code' => 'C02030030-',
	'type' => 'REF',
	'search_order' => '04-undefined',
	'price_EUR' => '255',
	'price_USD' => '240',
	'price_GBP' => '230',
	'price_JPY' => '39945',
	'price_CNY' => '',
	'price_AUD' => '600',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-bisulfite-kit-50-rxns',
	'meta_title' => 'Premium Bisulfite kit',
	'meta_keywords' => '',
	'meta_description' => 'Premium Bisulfite kit',
	'modified' => '2023-04-20 16:13:50',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '180',
		'product_id' => '2287',
		'related_id' => '1892'
	),
	'Image' => array()
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '28',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'DB',
	'description' => '<p>Dot blotting</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'dot-blotting',
	'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
	'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
	'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 14:40:49',
	'created' => '2015-07-08 13:45:05',
	'ProductsApplication' => array(
		'id' => '1887',
		'product_id' => '2287',
		'application_id' => '28'
	)
)
$slugs = array(
	(int) 0 => 'dot-blotting'
)
$applications = array(
	'id' => '28',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'DB',
	'description' => '<p>Dot blotting</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'dot-blotting',
	'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
	'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
	'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 14:40:49',
	'created' => '2015-07-08 13:45:05',
	'locale' => 'jpn'
)
$description = '<p>Dot blotting</p>'
$name = 'DB'
$document = array(
	'id' => '11',
	'name' => 'Antibodies you can trust',
	'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
	'image_id' => null,
	'type' => 'Poster',
	'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
	'slug' => 'antibodies-you-can-trust-poster',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-10-01 20:18:31',
	'created' => '2015-07-03 16:05:15',
	'ProductsDocument' => array(
		'id' => '2144',
		'product_id' => '2287',
		'document_id' => '11'
	)
)
$sds = array(
	'id' => '3641',
	'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-FR-fr-GHS_2_0.pdf',
	'countries' => 'FR',
	'modified' => '2024-01-17 17:10:07',
	'created' => '2024-01-17 17:10:07',
	'ProductsSafetySheet' => array(
		'id' => '5941',
		'product_id' => '2287',
		'safety_sheet_id' => '3641'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '3-methylcytosine (3-mC),polyclonal antibody',
	'meta_description' => 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane',
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<div class="small-5 columns">
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<p><small><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.</small></p>
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<td>Dot Blotting</td>
<td>1:400</td>
<td>Fig 1</td>
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<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
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<div class="small-5 columns">
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<p><small><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.</small></p>
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<th>References</th>
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<td>1:400</td>
<td>Fig 1</td>
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<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
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			'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
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<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
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			'description' => '<p><a href="https://www.diagenode.com/files/products/kits/hMeDIP_kit_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><span>The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA<span><span>&nbsp;</span>samples for use in genome-wide methylation analysis. It features</span></span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA<span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</p>
<p><em>Looking for hMeDIP-seq protocol? <a href="https://go.diagenode.com/l/928883/2022-01-07/2m1ht" target="_blank" title="Contact us">Contact us</a></em></p>
<p><span></span></p>
<p><span></span></p>',
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<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>hmeDNA and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
<li>Improved single-tube, magnetic bead-based protocol</li>
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<p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p>
<h3><span>Features</span></h3>
<ul>
<li>Starting DNA amount: <strong>10 ng &ndash; 1 &micro;g</strong></li>
<li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li>
<li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li>
<li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li>
<li><strong>High reproducibility</strong> between replicates and repetitive experiments</li>
<li>Compatible with <strong>all species&nbsp;</strong></li>
</ul>
<p>&nbsp;</p>
<div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div>
<div class="small-12 medium-8 large-8 columns">
<h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3>
<h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3>
</div>
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<h3></h3>',
			'label1' => 'MagMeDIP workflow',
			'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p>
<h3><span>How it works</span></h3>
<p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP&rsquo;d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p>
<center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center>
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			'label2' => 'MeDIP-qPCR',
			'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode&rsquo;s IP-Star Compact Automated System (please refer to the kit manual).</p>
<ul>
<li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li>
<li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li>
<li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li>
<li><strong>Highly validated protocol</strong></li>
<li>Automated protocol supplied</li>
</ul>
<center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center>
<p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p>
<p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 &micro;g or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p>
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			'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p>
<ul style="list-style-type: circle;">
<li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 &mu;g). It can be a home-made solution or a commercial one.</li>
<li>Choose the indexing system that fits your needs considering the following features:</li>
<ul>
<ul>
<ul>
<li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li>
<li>Number of barcodes</li>
<li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li>
<li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li>
</ul>
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<li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li>
</ul>
<p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p>
<ul style="list-style-type: circle;">
<li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li>
</ul>
<ul style="list-style-type: circle;">
<li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li>
</ul>
<h3><span>MeDIP-seq workflow</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center>
<h3><span>Example of results</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1&mu;g &ndash; 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency &gt;98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 &mu;g (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p>
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<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
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<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
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<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
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			'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p>
<p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p>
<p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p>
<p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p>
<p>Check out the list below to see all proposed antibodies for DNA modifications.</p>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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			'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-FR-fr-GHS_2_0.pdf',
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	(int) 7 => 'FI',
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	(int) 9 => 'BE',
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	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
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        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
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<li>
    <div class="row">
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                         <a href="/jp/p/hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>hMeDIP kit x16 (monoclonal mouse antibody)個カートに追加。</p>

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                    'C02010031',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
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                <button class="alert small button expand" onclick="$(this).addToCart('hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010031',
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    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1882" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">hMeDIP kit x16 (monoclonal mouse antibody)</h6>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/magmedip-kit-x48-48-rxns"><img src="/img/product/kits/C02010021-magmedip-qpcr.jpg" alt="MagMeDIP qPCR Kit box" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010021</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1880" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>MagMeDIP Kit個カートに追加。</p>

        <div class="row">
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                <button class="alert small button expand" onclick="$(this).addToCart('MagMeDIP Kit',
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                     '750',
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                    '750',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="magmedip-kit-x48-48-rxns" data-reveal-id="cartModal-1880" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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<li>
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            <h6 style="height:60px">MethylCap kit</h6>
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                         <a href="/jp/p/premium-bisulfite-kit-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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                <span class="success label" style="">C02030030</span>
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            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
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<form action="/jp/carts/add/1892" id="CartAdd/1892Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1892" id="CartProductId"/>
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                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
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                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
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                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-bisulfite-kit-50-rxns" data-reveal-id="cartModal-1892" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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'
$related = array(
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	'antibody_id' => null,
	'name' => 'Premium Bisulfite kit',
	'description' => '<p style="text-align: center;"><a href="https://www.diagenode.com/files/products/kits/Premium_Bisulfite_kit_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '50 rxns',
	'catalog_number' => 'C02030030',
	'old_catalog_number' => '',
	'sf_code' => 'C02030030-',
	'type' => 'REF',
	'search_order' => '04-undefined',
	'price_EUR' => '255',
	'price_USD' => '240',
	'price_GBP' => '230',
	'price_JPY' => '39945',
	'price_CNY' => '',
	'price_AUD' => '600',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-bisulfite-kit-50-rxns',
	'meta_title' => 'Premium Bisulfite kit',
	'meta_keywords' => '',
	'meta_description' => 'Premium Bisulfite kit',
	'modified' => '2023-04-20 16:13:50',
	'created' => '2015-06-29 14:08:20',
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		'product_id' => '2287',
		'related_id' => '1892'
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	'Image' => array()
)
$rrbs_service = array(
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	(int) 1 => (int) 1895
)
$chipseq_service = array(
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	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '28',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'DB',
	'description' => '<p>Dot blotting</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'dot-blotting',
	'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
	'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
	'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 14:40:49',
	'created' => '2015-07-08 13:45:05',
	'ProductsApplication' => array(
		'id' => '1887',
		'product_id' => '2287',
		'application_id' => '28'
	)
)
$slugs = array(
	(int) 0 => 'dot-blotting'
)
$applications = array(
	'id' => '28',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'DB',
	'description' => '<p>Dot blotting</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'dot-blotting',
	'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
	'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
	'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 14:40:49',
	'created' => '2015-07-08 13:45:05',
	'locale' => 'jpn'
)
$description = '<p>Dot blotting</p>'
$name = 'DB'
$document = array(
	'id' => '11',
	'name' => 'Antibodies you can trust',
	'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
	'image_id' => null,
	'type' => 'Poster',
	'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
	'slug' => 'antibodies-you-can-trust-poster',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-10-01 20:18:31',
	'created' => '2015-07-03 16:05:15',
	'ProductsDocument' => array(
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		'product_id' => '2287',
		'document_id' => '11'
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)
$sds = array(
	'id' => '3641',
	'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-FR-fr-GHS_2_0.pdf',
	'countries' => 'FR',
	'modified' => '2024-01-17 17:10:07',
	'created' => '2024-01-17 17:10:07',
	'ProductsSafetySheet' => array(
		'id' => '5941',
		'product_id' => '2287',
		'safety_sheet_id' => '3641'
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)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '3-methylcytosine (3-mC),polyclonal antibody',
	'meta_description' => 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane',
	'meta_title' => '3-methylcytosine (3-mC) polyclonal antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2287',
			'antibody_id' => '301',
			'name' => '3-methylcytosine (3-mC) polyclonal antibody ',
			'description' => '<p><span>Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410209-Fig1-DotBlot.jpg" alt="Dot blot" height="145" width="400" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.</small></p>
</div>
</div>',
			'label2' => 'Target description',
			'info2' => '<p>3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '50 μg/28 μl',
			'catalog_number' => 'C15410209',
			'old_catalog_number' => '',
			'sf_code' => 'C15410209-D001-000581',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '260',
			'price_USD' => '260',
			'price_GBP' => '245',
			'price_JPY' => '40730',
			'price_CNY' => '',
			'price_AUD' => '650',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
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			'meta_description' => 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane',
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			'clonality' => '',
			'isotype' => '',
			'lot' => 'A2111-0010',
			'concentration' => '1.80 &micro;g/&micro;l',
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			'type' => 'Polyclonal',
			'purity' => 'Protein G purified',
			'classification' => 'Pioneer',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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</thead>
<tbody>
<tr>
<td>Dot Blotting</td>
<td>1:400</td>
<td>Fig 1</td>
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<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
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$meta_title = '3-methylcytosine (3-mC) polyclonal antibody | Diagenode'
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		'name' => '3-methylcytosine (3-mC) polyclonal antibody ',
		'description' => '<p><span>Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.</span></p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410209-Fig1-DotBlot.jpg" alt="Dot blot" height="145" width="400" /></p>
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<p><small><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.</small></p>
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		'slug' => '3-mc-polyclonal-antibody-pioneer-50-mg-28-ml',
		'meta_title' => '3-methylcytosine (3-mC) polyclonal antibody | Diagenode',
		'meta_keywords' => '3-methylcytosine (3-mC),polyclonal antibody',
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<th>Suggested dilution</th>
<th>References</th>
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</thead>
<tbody>
<tr>
<td>Dot Blotting</td>
<td>1:400</td>
<td>Fig 1</td>
</tr>
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<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
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			'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
</ul>
<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
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			'price_EUR' => '630',
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			'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'meta_keywords' => '',
			'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'modified' => '2021-01-18 10:37:19',
			'created' => '2015-06-29 14:08:20',
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			'id' => '1882',
			'antibody_id' => null,
			'name' => 'hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><a href="https://www.diagenode.com/files/products/kits/hMeDIP_kit_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><span>The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA<span><span>&nbsp;</span>samples for use in genome-wide methylation analysis. It features</span></span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA<span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</p>
<p><em>Looking for hMeDIP-seq protocol? <a href="https://go.diagenode.com/l/928883/2022-01-07/2m1ht" target="_blank" title="Contact us">Contact us</a></em></p>
<p><span></span></p>
<p><span></span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>hmeDNA and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
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			'name' => 'MagMeDIP Kit',
			'description' => '<p><a href="https://www.diagenode.com/files/products/kits/magmedip-kit-manual-C02010020-21.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p>
<h3><span>Features</span></h3>
<ul>
<li>Starting DNA amount: <strong>10 ng &ndash; 1 &micro;g</strong></li>
<li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li>
<li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li>
<li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li>
<li><strong>High reproducibility</strong> between replicates and repetitive experiments</li>
<li>Compatible with <strong>all species&nbsp;</strong></li>
</ul>
<p>&nbsp;</p>
<div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div>
<div class="small-12 medium-8 large-8 columns">
<h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3>
<h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3>
</div>
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<p></p>
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<h3></h3>',
			'label1' => 'MagMeDIP workflow',
			'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p>
<h3><span>How it works</span></h3>
<p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP&rsquo;d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p>
<center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center>
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			'label2' => 'MeDIP-qPCR',
			'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode&rsquo;s IP-Star Compact Automated System (please refer to the kit manual).</p>
<ul>
<li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li>
<li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li>
<li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li>
<li><strong>Highly validated protocol</strong></li>
<li>Automated protocol supplied</li>
</ul>
<center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center>
<p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p>
<p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 &micro;g or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p>
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			'label3' => 'MeDIP-seq',
			'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p>
<ul style="list-style-type: circle;">
<li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 &mu;g). It can be a home-made solution or a commercial one.</li>
<li>Choose the indexing system that fits your needs considering the following features:</li>
<ul>
<ul>
<ul>
<li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li>
<li>Number of barcodes</li>
<li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li>
<li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li>
</ul>
</ul>
</ul>
<li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li>
</ul>
<p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p>
<ul style="list-style-type: circle;">
<li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li>
</ul>
<ul style="list-style-type: circle;">
<li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li>
</ul>
<h3><span>MeDIP-seq workflow</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center>
<h3><span>Example of results</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1&mu;g &ndash; 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center>
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<p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency &gt;98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 &mu;g (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p>
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<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
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<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
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			'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p>
<p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p>
<p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p>
<p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p>
<p>Check out the list below to see all proposed antibodies for DNA modifications.</p>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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                         <a href="/jp/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



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                     '690',
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            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)',
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                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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        <div class="small-12 columns" >
            <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010031</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>hMeDIP kit x16 (monoclonal mouse antibody)個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010031',
                     '690',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('hMeDIP kit x16 (monoclonal mouse antibody)',
                    'C02010031',
                    '690',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1882" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">hMeDIP kit x16 (monoclonal mouse antibody)</h6>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/magmedip-kit-x48-48-rxns"><img src="/img/product/kits/C02010021-magmedip-qpcr.jpg" alt="MagMeDIP qPCR Kit box" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02010021</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1880" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1880" id="CartAdd/1880Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1880" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>MagMeDIP Kit個カートに追加。</p>

        <div class="row">
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                <button class="alert small button expand" onclick="$(this).addToCart('MagMeDIP Kit',
                    'C02010021',
                     '750',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('MagMeDIP Kit',
                    'C02010021',
                    '750',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="magmedip-kit-x48-48-rxns" data-reveal-id="cartModal-1880" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MagMeDIP qPCR kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/premium-bisulfite-kit-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030030</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1892" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1892" id="CartAdd/1892Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1892" id="CartProductId"/>
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Premium Bisulfite kit個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                     '240',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                    '240',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-bisulfite-kit-50-rxns" data-reveal-id="cartModal-1892" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium Bisulfite kit</h6>
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    </div>
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'
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	'id' => '1892',
	'antibody_id' => null,
	'name' => 'Premium Bisulfite kit',
	'description' => '<p style="text-align: center;"><a href="https://www.diagenode.com/files/products/kits/Premium_Bisulfite_kit_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '50 rxns',
	'catalog_number' => 'C02030030',
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	'type' => 'REF',
	'search_order' => '04-undefined',
	'price_EUR' => '255',
	'price_USD' => '240',
	'price_GBP' => '230',
	'price_JPY' => '39945',
	'price_CNY' => '',
	'price_AUD' => '600',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'premium-bisulfite-kit-50-rxns',
	'meta_title' => 'Premium Bisulfite kit',
	'meta_keywords' => '',
	'meta_description' => 'Premium Bisulfite kit',
	'modified' => '2023-04-20 16:13:50',
	'created' => '2015-06-29 14:08:20',
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		'product_id' => '2287',
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	'Image' => array()
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$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '28',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'DB',
	'description' => '<p>Dot blotting</p>',
	'in_footer' => false,
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	'online' => true,
	'tabular' => true,
	'slug' => 'dot-blotting',
	'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
	'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
	'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 14:40:49',
	'created' => '2015-07-08 13:45:05',
	'ProductsApplication' => array(
		'id' => '1887',
		'product_id' => '2287',
		'application_id' => '28'
	)
)
$slugs = array(
	(int) 0 => 'dot-blotting'
)
$applications = array(
	'id' => '28',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'DB',
	'description' => '<p>Dot blotting</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'dot-blotting',
	'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
	'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
	'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 14:40:49',
	'created' => '2015-07-08 13:45:05',
	'locale' => 'jpn'
)
$description = '<p>Dot blotting</p>'
$name = 'DB'
$document = array(
	'id' => '11',
	'name' => 'Antibodies you can trust',
	'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
	'image_id' => null,
	'type' => 'Poster',
	'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
	'slug' => 'antibodies-you-can-trust-poster',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-10-01 20:18:31',
	'created' => '2015-07-03 16:05:15',
	'ProductsDocument' => array(
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		'product_id' => '2287',
		'document_id' => '11'
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)
$sds = array(
	'id' => '3641',
	'name' => 'SDS C15410209 3-methylcytosine 3-mC Antibody FR fr',
	'language' => 'fr',
	'url' => 'files/SDS/3-mC/SDS-C15410209-3-methylcytosine_3-mC_Antibody-FR-fr-GHS_2_0.pdf',
	'countries' => 'FR',
	'modified' => '2024-01-17 17:10:07',
	'created' => '2024-01-17 17:10:07',
	'ProductsSafetySheet' => array(
		'id' => '5941',
		'product_id' => '2287',
		'safety_sheet_id' => '3641'
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)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '3-methylcytosine (3-mC),polyclonal antibody',
	'meta_description' => 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane',
	'meta_title' => '3-methylcytosine (3-mC) polyclonal antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2287',
			'antibody_id' => '301',
			'name' => '3-methylcytosine (3-mC) polyclonal antibody ',
			'description' => '<p><span>Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410209-Fig1-DotBlot.jpg" alt="Dot blot" height="145" width="400" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.</small></p>
</div>
</div>',
			'label2' => 'Target description',
			'info2' => '<p>3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '50 μg/28 μl',
			'catalog_number' => 'C15410209',
			'old_catalog_number' => '',
			'sf_code' => 'C15410209-D001-000581',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '260',
			'price_USD' => '260',
			'price_GBP' => '245',
			'price_JPY' => '40730',
			'price_CNY' => '',
			'price_AUD' => '650',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => '3-mc-polyclonal-antibody-pioneer-50-mg-28-ml',
			'meta_title' => '3-methylcytosine (3-mC) polyclonal antibody | Diagenode',
			'meta_keywords' => '3-methylcytosine (3-mC),polyclonal antibody',
			'meta_description' => 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane',
			'modified' => '2024-01-17 17:10:29',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '301',
			'name' => '3-mC polyclonal antibody',
			'description' => '3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A2111-0010',
			'concentration' => '1.80 &micro;g/&micro;l',
			'reactivity' => 'Human, mouse, other (wide range)',
			'type' => 'Polyclonal',
			'purity' => 'Protein G purified',
			'classification' => 'Pioneer',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Dot Blotting</td>
<td>1:400</td>
<td>Fig 1</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2016-08-03 15:14:19',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '301 - 3-mC polyclonal antibody (A2111-0010 - 1.80 &micro;g/&micro;l - Human, mouse, other (wide range) - Protein G purified - Rabbit)'
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
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		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
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$meta_keywords = '3-methylcytosine (3-mC),polyclonal antibody'
$meta_description = 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane'
$meta_title = '3-methylcytosine (3-mC) polyclonal antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2287',
		'antibody_id' => '301',
		'name' => '3-methylcytosine (3-mC) polyclonal antibody ',
		'description' => '<p><span>Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.</span></p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410209-Fig1-DotBlot.jpg" alt="Dot blot" height="145" width="400" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.</small></p>
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		'label2' => 'Target description',
		'info2' => '<p>3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.</p>',
		'label3' => '',
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		'format' => '50 μg/28 μl',
		'catalog_number' => 'C15410209',
		'old_catalog_number' => '',
		'sf_code' => 'C15410209-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '260',
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		'price_CNY' => '',
		'price_AUD' => '650',
		'country' => 'ALL',
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		'slug' => '3-mc-polyclonal-antibody-pioneer-50-mg-28-ml',
		'meta_title' => '3-methylcytosine (3-mC) polyclonal antibody | Diagenode',
		'meta_keywords' => '3-methylcytosine (3-mC),polyclonal antibody',
		'meta_description' => 'Using Diagenode's Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC), a Dot Blot analysis using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane',
		'modified' => '2024-01-17 17:10:29',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '301',
		'name' => '3-mC polyclonal antibody',
		'description' => '3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A2111-0010',
		'concentration' => '1.80 &micro;g/&micro;l',
		'reactivity' => 'Human, mouse, other (wide range)',
		'type' => 'Polyclonal',
		'purity' => 'Protein G purified',
		'classification' => 'Pioneer',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Dot Blotting</td>
<td>1:400</td>
<td>Fig 1</td>
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<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2016-08-03 15:14:19',
		'created' => '0000-00-00 00:00:00',
		'select_label' => '301 - 3-mC polyclonal antibody (A2111-0010 - 1.80 &micro;g/&micro;l - Human, mouse, other (wide range) - Protein G purified - Rabbit)'
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			'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA&nbsp;samples for use in genome-wide methylation analysis. It features</span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</span></p>',
			'label1' => ' Characteristics',
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<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
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<ul style="list-style-type: disc;">
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
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			'type' => 'RFR',
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			'price_EUR' => '630',
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			'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'meta_keywords' => '',
			'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)',
			'modified' => '2021-01-18 10:37:19',
			'created' => '2015-06-29 14:08:20',
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			'id' => '1882',
			'antibody_id' => null,
			'name' => 'hMeDIP kit x16 (monoclonal mouse antibody)',
			'description' => '<p><a href="https://www.diagenode.com/files/products/kits/hMeDIP_kit_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p><span>The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented&nbsp;genomic DNA<span><span>&nbsp;</span>samples for use in genome-wide methylation analysis. It features</span></span><span>&nbsp;a highly specific monoclonal antibody against&nbsp;</span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA<span>. It includes&nbsp;control DNA and primers to assess the effiency of the assay.&nbsp;</span>Performing hydroxymethylation profiling with the hMeDIP kit is fast,&nbsp;reliable and&nbsp;highly specific.</p>
<p><em>Looking for hMeDIP-seq protocol? <a href="https://go.diagenode.com/l/928883/2022-01-07/2m1ht" target="_blank" title="Contact us">Contact us</a></em></p>
<p><span></span></p>
<p><span></span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul style="list-style-type: disc;">
<li><span>Robust enrichment &amp; immunoprecipitation of hydroxymethylated DNA</span></li>
<li>Highly specific monoclonal antibody against 5-hmC<span>&nbsp;for reliable, reproducible results</span></li>
<li>Including&nbsp;control DNA and primers to&nbsp;<span>monitor the&nbsp;efficiency of the assay</span>
<ul style="list-style-type: circle;">
<li>hmeDNA and unmethylated DNA sequences and primer pairs</li>
<li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li>
</ul>
</li>
<li>Improved single-tube, magnetic bead-based protocol</li>
</ul>',
			'label2' => '',
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			'slug' => 'hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns',
			'meta_title' => 'hMeDIP kit x16 (monoclonal mouse antibody)',
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			'name' => 'MagMeDIP Kit',
			'description' => '<p><a href="https://www.diagenode.com/files/products/kits/magmedip-kit-manual-C02010020-21.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p>
<h3><span>Features</span></h3>
<ul>
<li>Starting DNA amount: <strong>10 ng &ndash; 1 &micro;g</strong></li>
<li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li>
<li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li>
<li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li>
<li><strong>High reproducibility</strong> between replicates and repetitive experiments</li>
<li>Compatible with <strong>all species&nbsp;</strong></li>
</ul>
<p>&nbsp;</p>
<div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div>
<div class="small-12 medium-8 large-8 columns">
<h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3>
<h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3>
</div>
<p></p>
<p></p>
<p></p>
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<h3></h3>',
			'label1' => 'MagMeDIP workflow',
			'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p>
<h3><span>How it works</span></h3>
<p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP&rsquo;d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p>
<center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center>
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			'label2' => 'MeDIP-qPCR',
			'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode&rsquo;s IP-Star Compact Automated System (please refer to the kit manual).</p>
<ul>
<li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li>
<li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li>
<li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li>
<li><strong>Highly validated protocol</strong></li>
<li>Automated protocol supplied</li>
</ul>
<center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center>
<p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p>
<p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 &micro;g or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p>
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			'label3' => 'MeDIP-seq',
			'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p>
<ul style="list-style-type: circle;">
<li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 &mu;g). It can be a home-made solution or a commercial one.</li>
<li>Choose the indexing system that fits your needs considering the following features:</li>
<ul>
<ul>
<ul>
<li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li>
<li>Number of barcodes</li>
<li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li>
<li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li>
</ul>
</ul>
</ul>
<li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li>
</ul>
<p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p>
<ul style="list-style-type: circle;">
<li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li>
</ul>
<ul style="list-style-type: circle;">
<li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li>
</ul>
<h3><span>MeDIP-seq workflow</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center>
<h3><span>Example of results</span></h3>
<center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1&mu;g &ndash; 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency &gt;98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p>
<p></p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center>
<p></p>
<p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 &mu;g (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p>
<p></p>
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<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
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<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
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<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
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			'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p>
<p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p>
<p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p>
<p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p>
<p>Check out the list below to see all proposed antibodies for DNA modifications.</p>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Datasheet 3-mC C15410209 DATASHEET Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

SDS C15410209 3-methylcytosine 3-mC Antibody GB en	Download
SDS C15410209 3-methylcytosine 3-mC Antibody US en	Download
SDS C15410209 3-methylcytosine 3-mC Antibody BE nl	Download
SDS C15410209 3-methylcytosine 3-mC Antibody DE de	Download
SDS C15410209 3-methylcytosine 3-mC Antibody JP ja	Download
SDS C15410209 3-methylcytosine 3-mC Antibody ES es	Download
SDS C15410209 3-methylcytosine 3-mC Antibody BE fr	Download
SDS C15410209 3-methylcytosine 3-mC Antibody FR fr	Download

3-methylcytosine (3-mC) polyclonal antibody

How to properly cite this product in your work

Auto hMeDIP kit x16 (monoclonal mouse antibody)

hMeDIP kit x16 (monoclonal mouse antibody)

MagMeDIP qPCR kit

MethylCap kit

Premium Bisulfite kit

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