3-methylcytosine (3-mC) polyclonal antibody

Lot	A2111-0010
Concentration	1.80 µg/µl
Species reactivity	Human, mouse, other (wide range)
Type	Polyclonal
Purity	Protein G purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Dot Blotting	1:400	Fig 1

Dot blot

Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC
To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

Target description

3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.

应用

DB
Dot blotting Read more

文档

Datasheet 3-mC C15410209 DATASHEET Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

Safety sheets

SDS C15410209 3-methylcytosine 3-mC Antibody GB en	Download
SDS C15410209 3-methylcytosine 3-mC Antibody US en	Download
SDS C15410209 3-methylcytosine 3-mC Antibody BE nl	Download
SDS C15410209 3-methylcytosine 3-mC Antibody DE de	Download
SDS C15410209 3-methylcytosine 3-mC Antibody JP ja	Download
SDS C15410209 3-methylcytosine 3-mC Antibody ES es	Download
SDS C15410209 3-methylcytosine 3-mC Antibody BE fr	Download
SDS C15410209 3-methylcytosine 3-mC Antibody FR fr	Download

出版物

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m3C is a mitochondrial mRNA modification which promotes tumor progression
Audrey Penning et al.
Mitochondrial mRNA modifications are suggested to play a role in fine-tuning mitochondrial gene expression and function. However, the epitranscriptomic landscape of mitochondrial mRNA (mt-mRNA) remains poorly explored. Here, we uncover N3-methylcytosine (m3C) as a novel mt-mRNA that is catalyzed by METTL8, an enzyme...

3-methylcytosine (3-mC) polyclonal antibody

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