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D-Plex mRNA-seq Kit for Illumina

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Catalog Number
Format
Price
C05030033
24 rxns
$1,575.00

(Coming soon)

D-Plex mRNA-seq Library Preparation Kit is a tool designed for the study of the whole coding transcriptome. The kit is using the D-Plex technology to generate directionnal libraries for Illumina sequencing directly from total RNAs or mRNAs that has already been enriched by poly(A) selection.

The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low input amounts. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for low-abundance or rare transcripts.

D-Plex mRNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.

D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:

  • Product features

  • Indexes

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

  • Data analysis

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  •  資料
    D-Plex mRNA-seq Kit MANUAL
    		 Download
    D-Plex RNA-seq library preparation solutions FLYER
    		 Download
  •  出版物

    How to properly cite our product/service in your work

    We strongly recommend using this: D-Plex mRNA-seq Kit for Illumina (Hologic Diagenode Cat# C05030033). Click here to copy to clipboard.

    Using our products or services in your publication? Let us know!

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    Zúñiga-Hernández JM et al.
    Nutrient scarcity is a frequent adverse condition that organisms face during their development. This condition may lead to long-lasting effects on the metabolism and behaviour of adults due to developmental epigenetic modifications. Here, we show that reducing nutrient availability during larval development affects ...

    Nickel and Cobalt Induced Toxicity in Paramecium: TheCellular View to Environmental Toxicants
    Garza A. et al.
    Cobalt and Nickel show increasing pollution in our environment. We analyzed their combined toxicity and uptake mechanisms in the early food chain by investigation of bacteria and the ciliate Paramecium as a primary consumer. Exposing Paramecium to metals, we can dissect between phagocytotic uptake and phagocytosis i...

    Manganese-Induced Toxicity in : What Can We Learn from theTranscriptome?
    Nicolai Merle M et al.
    Manganese (Mn) is an essential ubiquitous transition metal and, when occupationally or environmentally overexposed, a well-known risk factor for several neurological pathologies. However, the molecular mechanisms underlying Mn-induced neurotoxicity are largely unknown. In this study, addressing RNA-Seq analysis, bio...

    A Chromosomal Duplication Encompassing Interleukin-33 Causes aNovel Hyper IgE Phenotype Characterized by EosinophilicEsophagitis and Generalized Autoimmunity.
    Marwaha A.K. et al.
    Interleukin (IL)-33 is a member of the IL-1 cytokine family and is secreted by fibroblasts, endothelial cells, and epithelial cells in tissues (lung, skin, and gastrointestinal) that are exposed to the environment. 1 IL-33 is stored in the nucleus and environmental allergens trigger RIPK1-caspase 8 ripoptosome activ...

    Functional microRNA targetome undergoes degeneration-induced shift inthe retina.
    Chu-Tan Joshua A et al.
    BACKGROUND: MicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases including age-related macular degeneration (AMD), acting as post-transcriptional gene suppressors through their association with argonaute 2 (AGO2) - a key member of the RNA Induced Silencing Complex (RISC)...

  •  関連商品
    • D-Plex Total RNA-seq Kit for Illumina
      C05030031
      D-Plex Total RNA-seq Kit for Illumina
    • Small RNA library preparation with UMI for Illumina
      C05030001
      D-Plex Small RNA-seq Library Prep Kit x24
    • Illumina index primers for D-Plex small RNA library preparation with UMI
      C05030021
      D-Plex Unique Dual Indexes for Illumina - Set A
    • Illumina index primers for D-Plex small RNA library preparation with UMI
      C05030022
      D-Plex Unique Dual Indexes for Illumina - Set B

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