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D-Plex mRNA-seq Kit for Illumina

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Catalog Number
Format
Price
C05030033
24 rxns
$1,575.00

Product Features

  • An innovative technology with template switching and UMIs
  • Ultra-sensitive methodology with low input capability down to 10 ng
  • High efficiency for greater gene detection and biomarker identification
  • Consistent gene expression profiling, optimal for gene signature definition
  • Precise measurement of strand orientation for anti-sense transcription detection

Download the manual

The D-Plex mRNA-seq Library Preparation Kit is a tool designed for gene expression analysis. The kit utilizes the D-Plex technology to generate directional libraries for Illumina sequencing directly from total RNAs.

The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from low input samples, down to 10 ng of total RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for gene expression analysis and discovery applications in the coding transcriptome.

The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been developped to capture  an increased number of genes for great discovery efficiency.

The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:

Greater gene discovery efficiency at low inputs

total RNA kit

D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.

High mappability to the coding transcriptome

total RNA diversity

D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.

Consistent, sensitive gene expression profiling

total RNA

Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.

Exceptional data concordance at low inputs

total RNA

D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.

  • Indexes

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

  • Data analysis

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  •  Documents
    D-Plex mRNA-seq Kit MANUAL
    		 Download
    D-Plex RNA-seq library preparation solutions FLYER
    		 Download
  •  Publications

    How to properly cite our product/service in your work

    We strongly recommend using this: D-Plex mRNA-seq Kit for Illumina (Hologic Diagenode Cat# C05030033). Click here to copy to clipboard.

    Using our products or services in your publication? Let us know!

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    Zúñiga-Hernández JM et al.
    Nutrient scarcity is a frequent adverse condition that organisms face during their development. This condition may lead to long-lasting effects on the metabolism and behaviour of adults due to developmental epigenetic modifications. Here, we show that reducing nutrient availability during larval development affects ...

    Nickel and Cobalt Induced Toxicity in Paramecium: TheCellular View to Environmental Toxicants
    Garza A. et al.
    Cobalt and Nickel show increasing pollution in our environment. We analyzed their combined toxicity and uptake mechanisms in the early food chain by investigation of bacteria and the ciliate Paramecium as a primary consumer. Exposing Paramecium to metals, we can dissect between phagocytotic uptake and phagocytosis i...

    Manganese-Induced Toxicity in : What Can We Learn from theTranscriptome?
    Nicolai Merle M et al.
    Manganese (Mn) is an essential ubiquitous transition metal and, when occupationally or environmentally overexposed, a well-known risk factor for several neurological pathologies. However, the molecular mechanisms underlying Mn-induced neurotoxicity are largely unknown. In this study, addressing RNA-Seq analysis, bio...

    A Chromosomal Duplication Encompassing Interleukin-33 Causes aNovel Hyper IgE Phenotype Characterized by EosinophilicEsophagitis and Generalized Autoimmunity.
    Marwaha A.K. et al.
    Interleukin (IL)-33 is a member of the IL-1 cytokine family and is secreted by fibroblasts, endothelial cells, and epithelial cells in tissues (lung, skin, and gastrointestinal) that are exposed to the environment. 1 IL-33 is stored in the nucleus and environmental allergens trigger RIPK1-caspase 8 ripoptosome activ...

    Functional microRNA targetome undergoes degeneration-induced shift inthe retina.
    Chu-Tan Joshua A et al.
    BACKGROUND: MicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases including age-related macular degeneration (AMD), acting as post-transcriptional gene suppressors through their association with argonaute 2 (AGO2) - a key member of the RNA Induced Silencing Complex (RISC)...

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