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Other names: MLLT3, YEATS3
Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.
| Lot | A1474-001 |
|---|---|
| Concentration | Not determined |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal ChIP grade, ChIP-seq grade |
| Purity | Whole antiserum from rabbit containing 0.05% azide. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution/amount | References |
|---|---|---|
| ChIP/ChIP-seq * | 2 µl/ChIP | Fig 1, 2 |
| ELISA | 1:100 - 1:500 | Fig 3 |
*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9
ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9
ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).
Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.
AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.
| AF9 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a... | Download |
| SDS C15310266 AF9 Antibody GB en | Download |
| SDS C15310266 AF9 Antibody US en | Download |
| SDS C15310266 AF9 Antibody BE nl | Download |
| SDS C15310266 AF9 Antibody BE fr | Download |
| SDS C15310266 AF9 Antibody FR fr | Download |
| SDS C15310266 AF9 Antibody ES es | Download |
| SDS C15310266 AF9 Antibody DE de | Download |
| SDS C15310266 AF9 Antibody JP ja | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: AF9 Antibody (Diagenode Cat# C15310266 Lot# A1474-001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Sample size available.', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. 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ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. 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ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. 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Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '508', 'name' => 'AF9 polyclonal antibody', 'description' => 'AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1474-001', 'concentration' => 'Not determined', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution/amount</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>2 µl/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 14:30:49', 'created' => '2017-05-08 13:56:13', 'select_label' => '508 - AF9 polyclonal antibody (A1474-001 - Not determined - Human: positive. Other species: not tested. - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/af9-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.' $meta_title = 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode' $product = array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '508', 'name' => 'AF9 polyclonal antibody', 'description' => 'AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1474-001', 'concentration' => 'Not determined', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution/amount</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>2 µl/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 14:30:49', 'created' => '2017-05-08 13:56:13', 'select_label' => '508 - AF9 polyclonal antibody (A1474-001 - Not determined - Human: positive. Other species: not tested. - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '219', 'name' => 'C15310266', 'product_id' => '2907', 'modified' => '2017-10-12 15:55:58', 'created' => '2017-10-12 15:55:58' ) ), 'Group' => array( 'Group' => array( 'id' => '219', 'name' => 'C15310266', 'product_id' => '2907', 'modified' => '2017-10-12 15:55:58', 'created' => '2017-10-12 15:55:58' ), 'Master' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '508', 'name' => 'AF9 polyclonal antibody', 'description' => 'AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1474-001', 'concentration' => 'Not determined', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution/amount</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>2 µl/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.' $meta_title = 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode' $product = array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '508', 'name' => 'AF9 polyclonal antibody', 'description' => 'AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1474-001', 'concentration' => 'Not determined', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution/amount</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>2 µl/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 14:30:49', 'created' => '2017-05-08 13:56:13', 'select_label' => '508 - AF9 polyclonal antibody (A1474-001 - Not determined - Human: positive. Other species: not tested. - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '219', 'name' => 'C15310266', 'product_id' => '2907', 'modified' => '2017-10-12 15:55:58', 'created' => '2017-10-12 15:55:58' ) ), 'Group' => array( 'Group' => array( 'id' => '219', 'name' => 'C15310266', 'product_id' => '2907', 'modified' => '2017-10-12 15:55:58', 'created' => '2017-10-12 15:55:58' ), 'Master' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '969', 'name' => 'AF9 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLHconjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet-af9-C15310266.pdf', 'slug' => 'datasheet_heralpha_C15100066.pdf', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-10-12 16:16:12', 'created' => '2017-10-12 14:52:53', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3447', 'name' => 'SDS C15310266 AF9 Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/AF9/SDS-C15310266-AF9_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-16 13:11:59', 'created' => '2024-01-16 13:11:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3441', 'name' => 'SDS C15310266 AF9 Antibody US en', 'language' => 'en', 'url' => 'files/SDS/AF9/SDS-C15310266-AF9_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-16 13:09:58', 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. 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Other species: not tested.', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution/amount</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>2 µl/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.' $meta_title = 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode' $product = array( 'Product' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. 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ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. 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By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '508', 'name' => 'AF9 polyclonal antibody', 'description' => 'AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1474-001', 'concentration' => 'Not determined', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution/amount</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>2 µl/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 14:30:49', 'created' => '2017-05-08 13:56:13', 'select_label' => '508 - AF9 polyclonal antibody (A1474-001 - Not determined - Human: positive. Other species: not tested. - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '219', 'name' => 'C15310266', 'product_id' => '2907', 'modified' => '2017-10-12 15:55:58', 'created' => '2017-10-12 15:55:58' ) ), 'Group' => array( 'Group' => array( 'id' => '219', 'name' => 'C15310266', 'product_id' => '2907', 'modified' => '2017-10-12 15:55:58', 'created' => '2017-10-12 15:55:58' ), 'Master' => array( 'id' => '2907', 'antibody_id' => '508', 'name' => 'AF9 Antibody', 'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p> <p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310266', 'old_catalog_number' => '', 'sf_code' => 'C15310266-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-100-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR and ELISA. 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Sample size available.', 'modified' => '2024-01-16 13:12:49', 'created' => '2017-10-12 14:51:18' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. 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Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Sample size available.', 'modified' => '2024-01-16 13:13:18', 'created' => '2017-10-12 16:10:59', 'ProductsGroup' => array( 'id' => '244', 'product_id' => '2909', 'group_id' => '219' ) ) ) $pro = array( 'id' => '2909', 'antibody_id' => '508', 'name' => 'AF9 Antibody (sample size)', 'description' => '', 'label1' => 'Validation Data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310266-20', 'old_catalog_number' => '', 'sf_code' => 'C15310266-361', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '9500', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-20-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ELISA, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:13:18', 'created' => '2017-10-12 16:10:59', 'ProductsGroup' => array( 'id' => '244', 'product_id' => '2909', 'group_id' => '219' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '2909', 'antibody_id' => '508', 'name' => 'AF9 Antibody (sample size)', 'description' => '', 'label1' => 'Validation Data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310266-20', 'old_catalog_number' => '', 'sf_code' => 'C15310266-361', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '9500', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => 'May 4, 2017', 'slug' => 'af9-polyclonal-antibody-20-ul', 'meta_title' => 'AF9 Antibody - ChIP-seq Grade (C15310266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ELISA, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.', 'modified' => '2024-01-16 13:13:18', 'created' => '2017-10-12 16:10:59', 'ProductsGroup' => array( 'id' => '244', 'product_id' => '2909', 'group_id' => '219' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4692', 'product_id' => '2907', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '969', 'name' => 'AF9 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLHconjugated synthetic peptide containing a sequence from the central region of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet-af9-C15310266.pdf', 'slug' => 'datasheet_heralpha_C15100066.pdf', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-10-12 16:16:12', 'created' => '2017-10-12 14:52:53', 'ProductsDocument' => array( 'id' => '2431', 'product_id' => '2907', 'document_id' => '969' ) ) $sds = array( 'id' => '3448', 'name' => 'SDS C15310266 AF9 Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/AF9/SDS-C15310266-AF9_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 13:12:19', 'created' => '2024-01-16 13:12:19', 'ProductsSafetySheet' => array( 'id' => '5586', 'product_id' => '2907', 'safety_sheet_id' => '3448' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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