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Blue ladder - HRP monoclonal antibody

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Catalog Number

Format

Price

C15200202-100

100 µl

$190.00
Bulk order

Other format

C15200202-10

10 µl

The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.

Lot	003D
Concentration	not determined
Species reactivity	Specific for blue colour used in protein MW markers
Type	Monoclonal
Purity	Protein A purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:1,000 - 1:5,000	Fig 1, 2, 3

Validation data

Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol.

Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis.

Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested.

資料
Blue ladder - HRP Monoclonal Antibody DATASHEET
Monoclonal antibody raised in mouse against the blue colour used in many prestained prote... Download

出版物

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Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis
Schüchner S et al.
Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the uns...

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<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p><span></span></p>
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</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => 'View accessories & consumables for Bioruptor<sup>&reg;</sup> Plus',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
			'format' => '1 unit',
			'catalog_number' => 'B01020001',
			'old_catalog_number' => 'UCD-300 TM',
			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
			'price_EUR' => '22500',
			'price_USD' => '29500',
			'price_GBP' => '19500',
			'price_JPY' => '3524625',
			'price_CNY' => '/',
			'price_AUD' => '73750',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => true,
			'in_stock' => false,
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			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bioruptor-plus-sonication-device',
			'meta_title' => 'Bioruptor® Plus sonication device',
			'meta_keywords' => '',
			'meta_description' => 'Bioruptor® Plus sonication device',
			'modified' => '2024-08-22 15:29:44',
			'created' => '2015-06-29 14:08:20',
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			'Image' => array(
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		),
		(int) 1 => array(
			'id' => '2618',
			'antibody_id' => null,
			'name' => 'Protein Extraction beads',
			'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode&rsquo;s Bioruptors&reg; used in combination with the Protein Extraction beads (diameter &lt; 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<p><span>The Bioruptor&reg; Standard, Plus or Pico used in combinaton with the Protein Extraction&nbsp;</span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p>
<ul>
<li>Fast and simple</li>
<li>No contamination between samples</li>
<li>Efficient</li>
<li>Gentle processing</li>
<li>Reproducible</li>
<li>Temperature controlled</li>
<li>Multiplexing capability</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '20 g',
			'catalog_number' => 'C20000021',
			'old_catalog_number' => 'AL-003-0020',
			'sf_code' => 'C20000021-',
			'type' => 'REF',
			'search_order' => '01-Accessory',
			'price_EUR' => '95',
			'price_USD' => '100',
			'price_GBP' => '90',
			'price_JPY' => '14880',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'protein-extraction-beads-20-g',
			'meta_title' => 'Protein Extraction beads',
			'meta_keywords' => '',
			'meta_description' => 'Protein Extraction beads',
			'modified' => '2020-05-27 14:42:40',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
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			'Image' => array(
				[maximum depth reached]
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		),
		(int) 2 => array(
			'id' => '2617',
			'antibody_id' => null,
			'name' => 'Protein Extraction kit for Bioruptor<sup>&reg;</sup> Plus',
			'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode&rsquo;s&nbsp;</span>Bioruptor<sup>&reg;</sup> Plus<span>&nbsp;used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C20000020',
			'old_catalog_number' => 'AL-002-0050',
			'sf_code' => 'C20000020-',
			'type' => 'REF',
			'search_order' => '01-Accessory',
			'price_EUR' => '325',
			'price_USD' => '435',
			'price_GBP' => '290',
			'price_JPY' => '50910',
			'price_CNY' => '',
			'price_AUD' => '1088',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'protein-extraction-kit-50-rxns',
			'meta_title' => 'Protein Extraction kit',
			'meta_keywords' => '',
			'meta_description' => 'Protein Extraction kit',
			'modified' => '2018-01-26 10:56:35',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '33',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Other antibodies',
			'description' => '',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'other-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-04 16:02:57',
			'created' => '2014-12-25 12:45:50',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'slug' => 'all-antibodies',
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			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
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	),
	'Document' => array(
		(int) 0 => array(
			'id' => '685',
			'name' => 'Blue ladder - HRP Monoclonal Antibody',
			'description' => '<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_Blue_ladder_HRP.pdf',
			'slug' => 'datasheet-blue-ladder-hrp',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-08-03 14:37:35',
			'created' => '2015-07-07 11:47:44',
			'ProductsDocument' => array(
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		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
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	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3035',
			'name' => 'Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis',
			'authors' => 'Schüchner S et al.',
			'description' => '<p>Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the unsatisfying situation that the signal for the protein of interest and the signal for the markers are not captured simultaneously and have to be merged in an error-prone step. To allow the simultaneous detection of marker proteins we generated monoclonal antibodies specific for the protein dyes. To elicit a dye rather than protein specific immune response we immunized mice sequentially with dye-carrier protein complexes, in which a new carrier protein was used for each subsequent immunization. Moreover, by sequentially immunizing with dye-carrier protein complexes, in which different but structurally related dyes were used, we could also generate an antibody, termed anti-RAINBOW, that cross-reacted even with structurally related dyes not used in the immunizations. Our novel antibodies represent convenient tools for the simultaneous Western blot detection of commercially available prestained marker proteins in combination with the detection of any specific protein of interest. These antibodies will render obsolete the anachronistic tradition of manually charting marker bands on film.</p>',
			'date' => '2016-08-17',
			'pmid' => 'http://www.nature.com/articles/srep31363',
			'doi' => '',
			'modified' => '2016-09-23 16:40:33',
			'created' => '2016-09-23 16:40:33',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1038',
			'name' => 'Blue ladder HRP antibody SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/Blue_ladder/SDS-C15200202-Blue_ladder_-_HRP_Antibody-GB-en-GHS_3_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-01-26 11:02:20',
			'created' => '2021-01-26 11:02:20',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
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		'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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	'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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		'modified' => '2022-01-05 15:19:03',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '320',
		'name' => 'Blue ladder - HRP monoclonal antibody',
		'description' => 'Most prestained protein MW markers used in PAGE contain fragments that are labelled with a blue dye. This antibody specifically reacts with this blue dye, enabling to directly visualize the different marker fragments on the blot. This makes the positioning of the marker on the blot significantly more easy and accurate.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '003D',
		'concentration' => 'not determined',
		'reactivity' => 'Specific for blue colour used in protein MW markers',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:5,000</td>
<td>Fig 1, 2, 3</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2016-08-03 15:22:22',
		'created' => '0000-00-00 00:00:00',
		'select_label' => '320 - Blue ladder - HRP monoclonal antibody (003D - not determined - Specific for blue colour used in protein MW markers - Protein A purified - Mouse)'
	),
	'Slave' => array(
		(int) 0 => array(
			'id' => '6',
			'name' => 'C15200202',
			'product_id' => '2013',
			'modified' => '2016-02-17 17:43:22',
			'created' => '2016-02-17 17:43:22'
		)
	),
	'Group' => array(
		'Group' => array(
			'id' => '6',
			'name' => 'C15200202',
			'product_id' => '2013',
			'modified' => '2016-02-17 17:43:22',
			'created' => '2016-02-17 17:43:22'
		),
		'Master' => array(
			'id' => '2013',
			'antibody_id' => '320',
			'name' => 'Blue ladder - HRP Antibody',
			'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_2.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_fig3.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
</div>
</div>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15200202-100',
			'old_catalog_number' => '',
			'sf_code' => 'C15200202-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '205',
			'price_USD' => '190',
			'price_GBP' => '185',
			'price_JPY' => '32115',
			'price_CNY' => '',
			'price_AUD' => '475',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'blue-ladder-hrp-monoclonal-antibody-100-ul',
			'meta_title' => 'Blue ladder - HRP Monoclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'Blue ladder - HRP Monoclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available.',
			'modified' => '2022-01-05 15:19:03',
			'created' => '2015-06-29 14:08:20'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(
		(int) 0 => array(
			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p>
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</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => 'View accessories & consumables for Bioruptor<sup>&reg;</sup> Plus',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
			'format' => '1 unit',
			'catalog_number' => 'B01020001',
			'old_catalog_number' => 'UCD-300 TM',
			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
			'price_EUR' => '22500',
			'price_USD' => '29500',
			'price_GBP' => '19500',
			'price_JPY' => '3524625',
			'price_CNY' => '/',
			'price_AUD' => '73750',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => true,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bioruptor-plus-sonication-device',
			'meta_title' => 'Bioruptor® Plus sonication device',
			'meta_keywords' => '',
			'meta_description' => 'Bioruptor® Plus sonication device',
			'modified' => '2024-08-22 15:29:44',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2618',
			'antibody_id' => null,
			'name' => 'Protein Extraction beads',
			'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode&rsquo;s Bioruptors&reg; used in combination with the Protein Extraction beads (diameter &lt; 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<p><span>The Bioruptor&reg; Standard, Plus or Pico used in combinaton with the Protein Extraction&nbsp;</span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p>
<ul>
<li>Fast and simple</li>
<li>No contamination between samples</li>
<li>Efficient</li>
<li>Gentle processing</li>
<li>Reproducible</li>
<li>Temperature controlled</li>
<li>Multiplexing capability</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '20 g',
			'catalog_number' => 'C20000021',
			'old_catalog_number' => 'AL-003-0020',
			'sf_code' => 'C20000021-',
			'type' => 'REF',
			'search_order' => '01-Accessory',
			'price_EUR' => '95',
			'price_USD' => '100',
			'price_GBP' => '90',
			'price_JPY' => '14880',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'protein-extraction-beads-20-g',
			'meta_title' => 'Protein Extraction beads',
			'meta_keywords' => '',
			'meta_description' => 'Protein Extraction beads',
			'modified' => '2020-05-27 14:42:40',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2617',
			'antibody_id' => null,
			'name' => 'Protein Extraction kit for Bioruptor<sup>&reg;</sup> Plus',
			'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode&rsquo;s&nbsp;</span>Bioruptor<sup>&reg;</sup> Plus<span>&nbsp;used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C20000020',
			'old_catalog_number' => 'AL-002-0050',
			'sf_code' => 'C20000020-',
			'type' => 'REF',
			'search_order' => '01-Accessory',
			'price_EUR' => '325',
			'price_USD' => '435',
			'price_GBP' => '290',
			'price_JPY' => '50910',
			'price_CNY' => '',
			'price_AUD' => '1088',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'protein-extraction-kit-50-rxns',
			'meta_title' => 'Protein Extraction kit',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Phone number</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">City</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-1784">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d&#039;Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People&#039;s Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People&#039;s Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
    $('#country_selector_quote-1784').selectize();
</script><br />
    </div>
</div>
<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">State</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][state]" id="state-1784" maxlength="3" type="text"/><br />
    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/>    </div>
</div>
<div class="row collapse" id="email_v">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/>    </div>
</div>


<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Comment</span>
    </div>
    <div class="small-9 large-10 columns">
        <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea>    </div>
</div>

<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <div class="recaptcha"><div id="recaptcha6766f7ae1b95c"></div></div>    </div>
</div>
<br />

<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <button id="submit_btn-1784" class="alert button expand" form="Quote-1784" type="submit">Contact me</button>    </div>
</div>
</form><script>
    var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';

    function postToPardot(formAction, id) {
        $('#pardot-form-handler').load(function(){
            $('#Quote-' + id).attr('action', formAction);
            $('#Quote-' + id).submit();
        });
        $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
        
    }
    $(document).ready(function() {
        $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
		$('#Quote-1784').attr('action','javascript:postToPardot(\'' + $('#Quote-1784').attr('action') + '\', 1784)');
    });

    $("#Quote-1784 #submit_btn-1784").click(function (e) {
            
        if($(this).closest('form')[0].checkValidity()){
            e.preventDefault();
            
            //disable the submit button
            $("#Quote-1784 #submit_btn-1784").attr("disabled", true);
            $("#Quote-1784 #submit_btn-1784").html("Processing...");

            //submit the form
            $("#Quote-1784").submit();
        } 
    })
</script>
<script>
    if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'US') {
        var val = $("#state-1784").val();
        $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
        if (val.length == 2) {
            $("#state-1784").val(val);
        }
        $("#state-1784").parent().parent().show();
    } else if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'CA') {
        var val = $("#state-1784").val();
        $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
        if (val.length == 2) {
            $("#state-1784").val(val);
        }
        $("#state-1784").parent().parent().show();
    } else if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'DE') {
        var val = $("#state-1784").val();
        $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
        if (val.length == 2) {
            $("#state-1784").val(val);
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        $("#state-1784").parent().parent().show();
    } else {
        $("#Quote-1784 #state-1784").parent().parent().hide();
        $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">');
    }

    $("#Quote-1784 #country_selector_quote-1784.selectized").change(function() {
        if (this.value == 'US') {
            $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
            $("#Quote-1784 #state-1784").parent().parent().show();
        } else if (this.value == 'CA') {
            $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
            $("#Quote-1784 #state-1784").parent().parent().show();
        } else if (this.value == 'DE') {
            $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
            $("#Quote-1784 #state-1784").parent().parent().show();
        } else {
            $("#Quote-1784 #state-1784").parent().parent().hide();
            $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">');
        }

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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div class="small-4 columns">
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_2.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => 'View accessories & consumables for Bioruptor<sup>&reg;</sup> Plus',
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<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
</div>
</div>
</div>',
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			'description' => '<p>Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the unsatisfying situation that the signal for the protein of interest and the signal for the markers are not captured simultaneously and have to be merged in an error-prone step. To allow the simultaneous detection of marker proteins we generated monoclonal antibodies specific for the protein dyes. To elicit a dye rather than protein specific immune response we immunized mice sequentially with dye-carrier protein complexes, in which a new carrier protein was used for each subsequent immunization. Moreover, by sequentially immunizing with dye-carrier protein complexes, in which different but structurally related dyes were used, we could also generate an antibody, termed anti-RAINBOW, that cross-reacted even with structurally related dyes not used in the immunizations. Our novel antibodies represent convenient tools for the simultaneous Western blot detection of commercially available prestained marker proteins in combination with the detection of any specific protein of interest. These antibodies will render obsolete the anachronistic tradition of manually charting marker bands on film.</p>',
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		'id' => '2012',
		'antibody_id' => '320',
		'name' => 'Blue ladder - HRP Antibody',
		'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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		'meta_title' => 'Blue ladder - HRP Monoclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'Blue ladder - HRP Monoclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available.',
		'modified' => '2022-01-05 15:19:16',
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$pro = array(
	'id' => '2012',
	'antibody_id' => '320',
	'name' => 'Blue ladder - HRP Antibody',
	'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
	'label1' => 'Validation data',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<div class="small-4 columns">
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<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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	'format' => '10 µl',
	'catalog_number' => 'C15200202-10',
	'old_catalog_number' => '',
	'sf_code' => 'C15200202-19650',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '55',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => 'View accessories & consumables for Bioruptor<sup>&reg;</sup> Plus',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
			'format' => '1 unit',
			'catalog_number' => 'B01020001',
			'old_catalog_number' => 'UCD-300 TM',
			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
			'price_EUR' => '22500',
			'price_USD' => '29500',
			'price_GBP' => '19500',
			'price_JPY' => '3524625',
			'price_CNY' => '/',
			'price_AUD' => '73750',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => true,
			'in_stock' => false,
			'featured' => true,
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			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bioruptor-plus-sonication-device',
			'meta_title' => 'Bioruptor® Plus sonication device',
			'meta_keywords' => '',
			'meta_description' => 'Bioruptor® Plus sonication device',
			'modified' => '2024-08-22 15:29:44',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
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			'Image' => array(
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		),
		(int) 1 => array(
			'id' => '2618',
			'antibody_id' => null,
			'name' => 'Protein Extraction beads',
			'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode&rsquo;s Bioruptors&reg; used in combination with the Protein Extraction beads (diameter &lt; 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<p><span>The Bioruptor&reg; Standard, Plus or Pico used in combinaton with the Protein Extraction&nbsp;</span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p>
<ul>
<li>Fast and simple</li>
<li>No contamination between samples</li>
<li>Efficient</li>
<li>Gentle processing</li>
<li>Reproducible</li>
<li>Temperature controlled</li>
<li>Multiplexing capability</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '20 g',
			'catalog_number' => 'C20000021',
			'old_catalog_number' => 'AL-003-0020',
			'sf_code' => 'C20000021-',
			'type' => 'REF',
			'search_order' => '01-Accessory',
			'price_EUR' => '95',
			'price_USD' => '100',
			'price_GBP' => '90',
			'price_JPY' => '14880',
			'price_CNY' => '',
			'price_AUD' => '250',
			'country' => 'ALL',
			'except_countries' => 'None',
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			'slug' => 'protein-extraction-beads-20-g',
			'meta_title' => 'Protein Extraction beads',
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			'meta_description' => 'Protein Extraction beads',
			'modified' => '2020-05-27 14:42:40',
			'created' => '2015-06-29 14:08:20',
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			'Image' => array(
				[maximum depth reached]
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		(int) 2 => array(
			'id' => '2617',
			'antibody_id' => null,
			'name' => 'Protein Extraction kit for Bioruptor<sup>&reg;</sup> Plus',
			'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode&rsquo;s&nbsp;</span>Bioruptor<sup>&reg;</sup> Plus<span>&nbsp;used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C20000020',
			'old_catalog_number' => 'AL-002-0050',
			'sf_code' => 'C20000020-',
			'type' => 'REF',
			'search_order' => '01-Accessory',
			'price_EUR' => '325',
			'price_USD' => '435',
			'price_GBP' => '290',
			'price_JPY' => '50910',
			'price_CNY' => '',
			'price_AUD' => '1088',
			'country' => 'ALL',
			'except_countries' => 'Japan',
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			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'protein-extraction-kit-50-rxns',
			'meta_title' => 'Protein Extraction kit',
			'meta_keywords' => '',
			'meta_description' => 'Protein Extraction kit',
			'modified' => '2018-01-26 10:56:35',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
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	'Application' => array(
		(int) 0 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
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			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '33',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Other antibodies',
			'description' => '',
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			'slug' => 'other-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-04 16:02:57',
			'created' => '2014-12-25 12:45:50',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'slug' => 'all-antibodies',
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			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
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	'Document' => array(
		(int) 0 => array(
			'id' => '685',
			'name' => 'Blue ladder - HRP Monoclonal Antibody',
			'description' => '<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_Blue_ladder_HRP.pdf',
			'slug' => 'datasheet-blue-ladder-hrp',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-08-03 14:37:35',
			'created' => '2015-07-07 11:47:44',
			'ProductsDocument' => array(
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		)
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	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '250',
			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
			'modified' => '2020-11-27 07:00:09',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
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	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3035',
			'name' => 'Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis',
			'authors' => 'Schüchner S et al.',
			'description' => '<p>Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the unsatisfying situation that the signal for the protein of interest and the signal for the markers are not captured simultaneously and have to be merged in an error-prone step. To allow the simultaneous detection of marker proteins we generated monoclonal antibodies specific for the protein dyes. To elicit a dye rather than protein specific immune response we immunized mice sequentially with dye-carrier protein complexes, in which a new carrier protein was used for each subsequent immunization. Moreover, by sequentially immunizing with dye-carrier protein complexes, in which different but structurally related dyes were used, we could also generate an antibody, termed anti-RAINBOW, that cross-reacted even with structurally related dyes not used in the immunizations. Our novel antibodies represent convenient tools for the simultaneous Western blot detection of commercially available prestained marker proteins in combination with the detection of any specific protein of interest. These antibodies will render obsolete the anachronistic tradition of manually charting marker bands on film.</p>',
			'date' => '2016-08-17',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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	'id' => '2012',
	'antibody_id' => '320',
	'name' => 'Blue ladder - HRP Antibody',
	'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_2.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 &mu;l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30&rdquo; exposure, a typical exposure time for WB analysis. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_fig3.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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	'format' => '10 µl',
	'catalog_number' => 'C15200202-10',
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	'sf_code' => 'C15200202-19650',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '55',
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	'price_JPY' => '8615',
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	'meta_title' => 'Blue ladder - HRP Monoclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'Blue ladder - HRP Monoclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available.',
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	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
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	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
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	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'jpn'
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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	'name' => 'Blue ladder - HRP Monoclonal Antibody',
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
</div>
</div>
</div>',
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	'type' => 'Datasheet',
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	'slug' => 'datasheet-blue-ladder-hrp',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-08-03 14:37:35',
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	'url' => 'files/SDS/Blue_ladder/SDS-C15200202-Blue_ladder_-_HRP_Antibody-ES-es-GHS_3_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-01-26 11:01:23',
	'created' => '2021-01-26 11:01:23',
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	'id' => '3035',
	'name' => 'Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis',
	'authors' => 'Schüchner S et al.',
	'description' => '<p>Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the unsatisfying situation that the signal for the protein of interest and the signal for the markers are not captured simultaneously and have to be merged in an error-prone step. To allow the simultaneous detection of marker proteins we generated monoclonal antibodies specific for the protein dyes. To elicit a dye rather than protein specific immune response we immunized mice sequentially with dye-carrier protein complexes, in which a new carrier protein was used for each subsequent immunization. Moreover, by sequentially immunizing with dye-carrier protein complexes, in which different but structurally related dyes were used, we could also generate an antibody, termed anti-RAINBOW, that cross-reacted even with structurally related dyes not used in the immunizations. Our novel antibodies represent convenient tools for the simultaneous Western blot detection of commercially available prestained marker proteins in combination with the detection of any specific protein of interest. These antibodies will render obsolete the anachronistic tradition of manually charting marker bands on film.</p>',
	'date' => '2016-08-17',
	'pmid' => 'http://www.nature.com/articles/srep31363',
	'doi' => '',
	'modified' => '2016-09-23 16:40:33',
	'created' => '2016-09-23 16:40:33',
	'ProductsPublication' => array(
		'id' => '1612',
		'product_id' => '2013',
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$externalLink = ' <a href="http://www.nature.com/articles/srep31363" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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