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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<div class="row">
<div class="small-4 columns">
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
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<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
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</div>',
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'id' => '2012',
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'name' => 'Blue ladder - HRP Antibody',
'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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'meta_description' => 'Blue ladder - HRP Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
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'name' => 'Blue ladder - HRP Antibody',
'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<h6 style="height:60px">Protein Extraction beads</h6>
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<div class="small-4 columns">
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
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</div>
</div>
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<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
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'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
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'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
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<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="section">
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
</div>
</div>
</div>',
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'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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</div>
<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
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<li>
<div class="row">
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<a href="/cn/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
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<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction beads</strong> 添加至我的购物车。</p>
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<h6 style="height:60px">Protein Extraction beads</h6>
</div>
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<li>
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<a href="/cn/p/protein-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
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<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction kit for Bioruptor<sup>®</sup> Plus</strong> 添加至我的购物车。</p>
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<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
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<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
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<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
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'
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'name' => 'Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s </span>Bioruptor<sup>®</sup> Plus<span> used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.</span></p>',
'label1' => '',
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'old_catalog_number' => 'AL-002-0050',
'sf_code' => 'C20000020-',
'type' => 'REF',
'search_order' => '01-Accessory',
'price_EUR' => '325',
'price_USD' => '435',
'price_GBP' => '290',
'price_JPY' => '50910',
'price_CNY' => '',
'price_AUD' => '1088',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'protein-extraction-kit-50-rxns',
'meta_title' => 'Protein Extraction kit',
'meta_keywords' => '',
'meta_description' => 'Protein Extraction kit',
'modified' => '2018-01-26 10:56:35',
'created' => '2015-06-29 14:08:20',
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'id' => '2408',
'product_id' => '2013',
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'modified' => '2019-04-09 12:06:37',
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(int) 0 => (int) 1894,
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(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
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$other_format = array(
'id' => '2012',
'antibody_id' => '320',
'name' => 'Blue ladder - HRP Antibody',
'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_2.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_fig3.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_fig3.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
</div>
</div>',
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<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
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'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
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'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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<div class="small-12 columns" >
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<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
</div>
</div>
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'name' => 'Blue ladder - HRP Antibody',
'description' => '<p>The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_1.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> 5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200202_WB_fig3.png" alt="" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong><br /> Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></p>
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'type' => 'FRE',
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'meta_title' => 'Blue ladder - HRP Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'Blue ladder - HRP Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:19:16',
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'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
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'position' => '10',
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'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'zho'
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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'name' => 'Blue ladder - HRP Monoclonal Antibody',
'description' => '<div class="page" title="Page 1">
<div class="section">
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<p><span>Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB. For easy detection, the antibody has been coupled to HRP omitting the need to use a secondary antibody. </span></p>
</div>
</div>
</div>
</div>',
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'type' => 'Datasheet',
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'slug' => 'datasheet-blue-ladder-hrp',
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'modified' => '2015-08-03 14:37:35',
'created' => '2015-07-07 11:47:44',
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'document_id' => '685'
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'name' => 'Blue ladder HRP antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Blue_ladder/SDS-C15200202-Blue_ladder_-_HRP_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 11:01:23',
'created' => '2021-01-26 11:01:23',
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'id' => '3035',
'name' => 'Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis',
'authors' => 'Schüchner S et al.',
'description' => '<p>Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the unsatisfying situation that the signal for the protein of interest and the signal for the markers are not captured simultaneously and have to be merged in an error-prone step. To allow the simultaneous detection of marker proteins we generated monoclonal antibodies specific for the protein dyes. To elicit a dye rather than protein specific immune response we immunized mice sequentially with dye-carrier protein complexes, in which a new carrier protein was used for each subsequent immunization. Moreover, by sequentially immunizing with dye-carrier protein complexes, in which different but structurally related dyes were used, we could also generate an antibody, termed anti-RAINBOW, that cross-reacted even with structurally related dyes not used in the immunizations. Our novel antibodies represent convenient tools for the simultaneous Western blot detection of commercially available prestained marker proteins in combination with the detection of any specific protein of interest. These antibodies will render obsolete the anachronistic tradition of manually charting marker bands on film.</p>',
'date' => '2016-08-17',
'pmid' => 'http://www.nature.com/articles/srep31363',
'doi' => '',
'modified' => '2016-09-23 16:40:33',
'created' => '2016-09-23 16:40:33',
'ProductsPublication' => array(
'id' => '1612',
'product_id' => '2013',
'publication_id' => '3035'
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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