Request a quote for a bulk order for CBX2 Antibody. Please fill out the form here below. Your local sales account manager will get in touch with you shortly and send you a quotation based on your requirements.
Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein1.
| Lot | A302-524A4 |
|---|---|
| Concentration | 100 μl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. |
| Host | Rabbit |
| Storage Conditions | Store at 4°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 1 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:1,000 | Fig 3 |
| IP | 6 μg per IP | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
1Manufactured by Bethyl Laboratories, Inc., Texas, USA
Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2
ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2
ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).
Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2
Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.
CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| CBX2 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide ... | Download |
| SDS C15410339 CBX2 Antibody US en | Download |
| SDS C15410339 CBX2 Antibody GB en | Download |
| SDS C15410339 CBX2 Antibody DE de | Download |
| SDS C15410339 CBX2 Antibody JP ja | Download |
| SDS C15410339 CBX2 Antibody BE nl | Download |
| SDS C15410339 CBX2 Antibody FR fr | Download |
| SDS C15410339 CBX2 Antibody BE fr | Download |
| SDS C15410339 CBX2 Antibody ES es | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: CBX2 Antibody (Diagenode Cat# C15410339 Lot# A302-524A4). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.' $meta_title = 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode' $product = array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ) ), 'Group' => array( 'Group' => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ), 'Master' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3765', 'name' => 'SDS C15410339 CBX2 Antibody US en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-17 19:07:53', 'created' => '2024-01-17 19:07:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3763', 'name' => 'SDS C15410339 CBX2 Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-17 19:07:20', 'created' => '2024-01-17 19:07:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3768', 'name' => 'SDS C15410339 CBX2 Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-17 19:08:43', 'created' => '2024-01-17 19:08:43', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3764', 'name' => 'SDS C15410339 CBX2 Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-17 19:07:36', 'created' => '2024-01-17 19:07:36', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3767', 'name' => 'SDS C15410339 CBX2 Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:26', 'created' => '2024-01-17 19:08:26', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3762', 'name' => 'SDS C15410339 CBX2 Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-17 19:07:03', 'created' => '2024-01-17 19:07:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3766', 'name' => 'SDS C15410339 CBX2 Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:11', 'created' => '2024-01-17 19:08:11', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) ) $pro = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4743', 'product_id' => '2918', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( 'id' => '2476', 'product_id' => '2918', 'document_id' => '976' ) ) $sds = array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( 'id' => '6157', 'product_id' => '2918', 'safety_sheet_id' => '3769' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.' $meta_title = 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode' $product = array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ) ), 'Group' => array( 'Group' => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ), 'Master' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3765', 'name' => 'SDS C15410339 CBX2 Antibody US en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-17 19:07:53', 'created' => '2024-01-17 19:07:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3763', 'name' => 'SDS C15410339 CBX2 Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-17 19:07:20', 'created' => '2024-01-17 19:07:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3768', 'name' => 'SDS C15410339 CBX2 Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-17 19:08:43', 'created' => '2024-01-17 19:08:43', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3764', 'name' => 'SDS C15410339 CBX2 Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-17 19:07:36', 'created' => '2024-01-17 19:07:36', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3767', 'name' => 'SDS C15410339 CBX2 Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:26', 'created' => '2024-01-17 19:08:26', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3762', 'name' => 'SDS C15410339 CBX2 Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-17 19:07:03', 'created' => '2024-01-17 19:07:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3766', 'name' => 'SDS C15410339 CBX2 Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:11', 'created' => '2024-01-17 19:08:11', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) ) $pro = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4743', 'product_id' => '2918', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( 'id' => '2476', 'product_id' => '2918', 'document_id' => '976' ) ) $sds = array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( 'id' => '6157', 'product_id' => '2918', 'safety_sheet_id' => '3769' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.' $meta_title = 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode' $product = array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ) ), 'Group' => array( 'Group' => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ), 'Master' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3765', 'name' => 'SDS C15410339 CBX2 Antibody US en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-17 19:07:53', 'created' => '2024-01-17 19:07:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3763', 'name' => 'SDS C15410339 CBX2 Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-17 19:07:20', 'created' => '2024-01-17 19:07:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3768', 'name' => 'SDS C15410339 CBX2 Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-17 19:08:43', 'created' => '2024-01-17 19:08:43', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3764', 'name' => 'SDS C15410339 CBX2 Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-17 19:07:36', 'created' => '2024-01-17 19:07:36', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3767', 'name' => 'SDS C15410339 CBX2 Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:26', 'created' => '2024-01-17 19:08:26', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3762', 'name' => 'SDS C15410339 CBX2 Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-17 19:07:03', 'created' => '2024-01-17 19:07:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3766', 'name' => 'SDS C15410339 CBX2 Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:11', 'created' => '2024-01-17 19:08:11', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) ) $pro = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4743', 'product_id' => '2918', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( 'id' => '2476', 'product_id' => '2918', 'document_id' => '976' ) ) $sds = array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( 'id' => '6157', 'product_id' => '2918', 'safety_sheet_id' => '3769' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.' $meta_title = 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode' $product = array( 'Product' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '513', 'name' => 'CBX2 polyclonal antibody', 'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.', 'clonality' => '', 'isotype' => '', 'lot' => 'A302-524A4', 'concentration' => '100 μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-22 12:00:12', 'created' => '2017-12-21 10:03:19', 'select_label' => '513 - CBX2 polyclonal antibody (A302-524A4 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ) ), 'Group' => array( 'Group' => array( 'id' => '340', 'name' => 'C15410339', 'product_id' => '2918', 'modified' => '2020-12-30 13:53:05', 'created' => '2020-12-30 13:50:01' ), 'Master' => array( 'id' => '2918', 'antibody_id' => '513', 'name' => 'CBX2 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410339', 'old_catalog_number' => '', 'sf_code' => 'C15410339-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'June 20, 2017', 'slug' => 'cbx2-polyclonal-antibody-100-ul', 'meta_title' => 'CBX2 Antibody - ChIP-seq Grade (C15410339) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.', 'modified' => '2024-01-17 19:09:34', 'created' => '2017-12-21 10:01:25' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-proteins-histone-transferase', 'cookies_tag_id' => null, 'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes', 'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.', 'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:15', 'created' => '2014-06-26 13:18:41', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3765', 'name' => 'SDS C15410339 CBX2 Antibody US en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-17 19:07:53', 'created' => '2024-01-17 19:07:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3763', 'name' => 'SDS C15410339 CBX2 Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-17 19:07:20', 'created' => '2024-01-17 19:07:20', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3768', 'name' => 'SDS C15410339 CBX2 Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-17 19:08:43', 'created' => '2024-01-17 19:08:43', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3764', 'name' => 'SDS C15410339 CBX2 Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-17 19:07:36', 'created' => '2024-01-17 19:07:36', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3767', 'name' => 'SDS C15410339 CBX2 Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:26', 'created' => '2024-01-17 19:08:26', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3762', 'name' => 'SDS C15410339 CBX2 Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-17 19:07:03', 'created' => '2024-01-17 19:07:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3766', 'name' => 'SDS C15410339 CBX2 Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 19:08:11', 'created' => '2024-01-17 19:08:11', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/cbx2-polyclonal-antibody-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) ) $pro = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3177', 'antibody_id' => '638', 'name' => 'CBX2 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410339-20', 'old_catalog_number' => '', 'sf_code' => 'C15410339-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'cbx2-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-17 19:09:57', 'created' => '2020-12-30 13:49:36', 'ProductsGroup' => array( 'id' => '381', 'product_id' => '3177', 'group_id' => '340' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4743', 'product_id' => '2918', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'eng' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '976', 'name' => 'CBX2 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf', 'slug' => 'cbx2-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 10:23:27', 'created' => '2018-01-03 10:23:27', 'ProductsDocument' => array( 'id' => '2476', 'product_id' => '2918', 'document_id' => '976' ) ) $sds = array( 'id' => '3769', 'name' => 'SDS C15410339 CBX2 Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-17 19:09:03', 'created' => '2024-01-17 19:09:03', 'ProductsSafetySheet' => array( 'id' => '6157', 'product_id' => '2918', 'safety_sheet_id' => '3769' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.
