Diagenode

H3K27ac monoclonal antibody

Catalog Number
Format
Price
C15210016
100 µg
$440.00
  Bulk order



Monoclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.

Lot006
Concentration1 μg/μl
Species reactivityHuman, wide range expected.
TypeMonoclonal ChIP grade/ChIP-seq grade
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 50% glycerol, 1% BSA and 0.09% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 0.5 - 1 µg per IP Fig 1
Dot Blotting 1:2,000 Fig 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:1,000 Fig 4

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.

  • Validation data
    H3K27ac Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K27ac
    ChIP was performed on sheared chromatin from 500000 HeLa cells using 0.5 µg of the Diagenode antibody against H3K4me3 (cat. No. C15410003) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of the human X-chromosome (figure 2A and B) and in two regions surrounding the GAPDH and EIF4A2 positive control genes, respectively (figure 2C and D).

    A. H3K4me2 Antibody Cut &

    B. H3K4me2 Antibody for ChIP-seq

    C. H3K4me2 Antibody for ChIP-seq assay

    D. H3K4me2 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H3K27ac
    ChIP was performed on HeLa cells using 0.5 µg of the Diagenode antibody against H3K4me2 (cat. No. C15410035). The IP'd DNA was analysed on an Illumina Hiseq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figure 2A and 2B) and in 2 chromosomal regions surrounding the ACTB and GAPDH positive control genes (figure 2C and D, respectively).

    H3K27ac Antibody validated in  Dot Blot

    Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K27ac
    To test the cross reactivity of the Diagenode antibody against H3K27ac (cat. No. C15210016), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

    H3K27ac Antibody validated in Western blot

    Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K27ac
    Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K27ac (cat. No. C15210016). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.

    H3K27ac Antibody validated in Immunofluorescence

    Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K27ac
    HeLa cells were stained with the Diagenode antibody against H3K27ac (cat. No. C15210016, red) diluted 1:1,000. Actin was stained with fluorescein phalladoin (green).

  •  実験手法
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
    ChIP-seq (ab)
    Read more
  •  資料
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet H3K27ac monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against the region of histone H3 containing the acetylated l...
    Download
  •  Safety sheets
    H3K27ac Antibody SDS GB en Download
    H3K27ac Antibody SDS US en Download
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  •  出版物

    How to properly cite this product in your work

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