日本語 | | | / 検索

MethylCap protein

Catalog Number

Format

Price

C02020012
(mbd-001-100)

100 µg

$565.00

メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。

資料
Datasheet MethylCap Protein DATASHEET
The MethylCap protein has been extensively validated for specific isolation of DNA fragments cont... Download

出版物

How to properly cite this product in your work

Diagenode strongly recommends using this: MethylCap protein (Diagenode Cat# C02020012). Click here to copy to clipboard.

Using our products in your publication? Let us know!

Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.
Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A
Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice st...

関連商品
- C02020011
  
  Auto MethylCap kit
- C03030020
  
  XL GenDNA Extraction Module
- B04000001
  
  DiaMag 0.2ml - マグネットラック
- C02020010
  
  MethylCap kit

Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap protein',
	'meta_title' => 'MethylCap protein',
	'product' => array(
		'Product' => array(
			'id' => '1889',
			'antibody_id' => null,
			'name' => 'MethylCap protein',
			'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C02020012',
			'old_catalog_number' => 'mbd-001-100',
			'sf_code' => 'C02020012-D001-000526',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '500',
			'price_USD' => '565',
			'price_GBP' => '460',
			'price_JPY' => '78325',
			'price_CNY' => '',
			'price_AUD' => '1412',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-protein-100-ug',
			'meta_title' => 'MethylCap protein',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap protein',
			'modified' => '2024-11-19 17:58:50',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'MethylCap protein'
$meta_title = 'MethylCap protein'
$product = array(
	'Product' => array(
		'id' => '1889',
		'antibody_id' => null,
		'name' => 'MethylCap protein',
		'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C02020012',
		'old_catalog_number' => 'mbd-001-100',
		'sf_code' => 'C02020012-D001-000526',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '500',
		'price_USD' => '565',
		'price_GBP' => '460',
		'price_JPY' => '78325',
		'price_CNY' => '',
		'price_AUD' => '1412',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'methylcap-protein-100-ug',
		'meta_title' => 'MethylCap protein',
		'meta_keywords' => '',
		'meta_description' => 'MethylCap protein',
		'modified' => '2024-11-19 17:58:50',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1922',
			'antibody_id' => null,
			'name' => 'XL GenDNA Extraction Module',
			'description' => '<p><span>This module has been designed for the extraction of <strong>60 &micro;g </strong>of<strong> genomic DNA</strong> from cultured cells. The protocol and reagents allow you to perform <strong>6 extractions</strong> of <strong>10 &micro;g</strong> of genomic DNA per sample, starting with&nbsp;1 to 1.5 million cells.</span></p>
<p><span>Please check our manual&nbsp;to scale&nbsp;your needs&nbsp;based on your starting material.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '6 rxns',
			'catalog_number' => 'C03030020',
			'old_catalog_number' => 'mc-magme-003',
			'sf_code' => 'C03030020-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '115',
			'price_USD' => '135',
			'price_GBP' => '105',
			'price_JPY' => '18015',
			'price_CNY' => '',
			'price_AUD' => '338',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'xl-gendna-extraction-module-6-rxns',
			'meta_title' => 'XL GenDNA Extraction Module',
			'meta_keywords' => '',
			'meta_description' => 'XL GenDNA Extraction Module',
			'modified' => '2019-04-11 14:22:10',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1819',
			'antibody_id' => null,
			'name' => 'DiaMag 0.2ml - magnetic rack',
			'description' => '<p>The DiaMag02 is a powerful magnet which has been designed for controlled and rapid isolation of your DNA bound to magnetic beads. It allows for processing 16 samples at a time.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '1 unit',
			'catalog_number' => 'B04000001',
			'old_catalog_number' => 'kch-816-001',
			'sf_code' => 'B04000001-',
			'type' => 'ACC',
			'search_order' => '04-undefined',
			'price_EUR' => '245',
			'price_USD' => '235',
			'price_GBP' => '215',
			'price_JPY' => '38380',
			'price_CNY' => '',
			'price_AUD' => '588',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'diamag02-magnetic-rack-1-unit',
			'meta_title' => 'DiaMag02 - magnetic rack',
			'meta_keywords' => '',
			'meta_description' => 'DiaMag02 - magnetic rack',
			'modified' => '2019-06-11 16:27:35',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1887',
			'antibody_id' => null,
			'name' => 'MethylCap kit',
			'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020010',
			'old_catalog_number' => 'AF-100-0048',
			'sf_code' => 'C02020010-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-kit-x48-48-rxns',
			'meta_title' => 'MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap kit x48',
			'modified' => '2024-11-21 06:38:46',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '53',
			'position' => '4',
			'parent_id' => '12',
			'name' => 'Methylbinding domain protein',
			'description' => '<p>The MBD technology used in our <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> is based on the very high affinity of&nbsp;the <a href="https://www.diagenode.com/en/p/methylcap-protein-100-ug">MethylCap protein</a>&nbsp;for methylated DNA. This protein consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag.&nbsp;</p>
<p>Diagenode&rsquo;s <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the <a href="https://www.diagenode.com/en/p/bioruptorpico2">Bioruptor&reg; sonication device</a>.</p>
<h2>How it works</h2>
<center><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/methyl_binding_domain_overview.jpg" /></center>
<h3 class="diacol">ADVANTAGES</h3>
<ul style="font-size: 19px;" class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> &amp; <strong>reproducible</strong> technique</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'methylbinding-domain-protein-assay',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Methylbinding domain protein,DNA methylation',
			'meta_description' => 'Diagenode's High affinity protein-based isolation of methylated DNA for Methylbinding domain protein assay',
			'meta_title' => 'Methylbinding domain protein assay for DNA methylation | Diagenode',
			'modified' => '2020-09-17 13:24:55',
			'created' => '2015-07-08 09:36:55',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '102',
			'name' => 'Datasheet MethylCap Protein',
			'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
			'slug' => 'datasheet-methylcap-protein',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-09-17 13:07:11',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '2867',
			'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
			'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
			'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
			'date' => '2015-08-05',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
			'doi' => '10.1371/journal.pone.0132136',
			'modified' => '2016-03-23 09:56:34',
			'created' => '2016-03-23 09:56:34',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '3865',
			'name' => 'SDS C02020012 MethylCap Protein US en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2024-11-19 18:08:07',
			'created' => '2024-11-19 18:08:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3863',
			'name' => 'SDS C02020012 MethylCap Protein GB en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2024-11-19 18:06:33',
			'created' => '2024-11-19 18:06:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '3858',
			'name' => 'SDS C02020012 MethylCap Protein BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 17:59:07',
			'created' => '2024-11-19 17:59:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3862',
			'name' => 'SDS C02020012 MethylCap Protein FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-11-19 18:05:47',
			'created' => '2024-11-19 18:05:47',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3861',
			'name' => 'SDS C02020012 MethylCap Protein ES es',
			'language' => 'es',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-11-19 18:03:27',
			'created' => '2024-11-19 18:03:27',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3860',
			'name' => 'SDS C02020012 MethylCap Protein DE de',
			'language' => 'de',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-11-19 18:02:09',
			'created' => '2024-11-19 18:01:50',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3864',
			'name' => 'SDS C02020012 MethylCap Protein JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-11-19 18:07:21',
			'created' => '2024-11-19 18:07:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3859',
			'name' => 'SDS C02020012 MethylCap Protein BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 18:00:25',
			'created' => '2024-11-19 18:00:25',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto MethylCap kit個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/xl-gendna-extraction-module-6-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03030020</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1922" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1922" id="CartAdd/1922Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1922" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>XL GenDNA Extraction Module個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                     '135',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                    '135',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="xl-gendna-extraction-module-6-rxns" data-reveal-id="cartModal-1922" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">XL GenDNA Extraction Module</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/diamag02-magnetic-rack-1-unit"><img src="/img/product/reagents/diamag02.png" alt="some alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">B04000001</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1819" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1819" id="CartAdd/1819Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1819" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>DiaMag 0.2ml - magnetic rack個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                     '235',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                    '235',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="diamag02-magnetic-rack-1-unit" data-reveal-id="cartModal-1819" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">DiaMag 0.2ml - マグネットラック</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MethylCap kit</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1887',
	'antibody_id' => null,
	'name' => 'MethylCap kit',
	'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
	'label1' => 'Characteristics',
	'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '48 rxns',
	'catalog_number' => 'C02020010',
	'old_catalog_number' => 'AF-100-0048',
	'sf_code' => 'C02020010-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '740',
	'price_USD' => '695',
	'price_GBP' => '675',
	'price_JPY' => '115920',
	'price_CNY' => '',
	'price_AUD' => '1738',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'methylcap-kit-x48-48-rxns',
	'meta_title' => 'MethylCap kit x48',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap kit x48',
	'modified' => '2024-11-21 06:38:46',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '4379',
		'product_id' => '1889',
		'related_id' => '1887'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mbd-001-100)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '102',
	'name' => 'Datasheet MethylCap Protein',
	'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
	'slug' => 'datasheet-methylcap-protein',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-09-17 13:07:11',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '713',
		'product_id' => '1889',
		'document_id' => '102'
	)
)
$sds = array(
	'id' => '3859',
	'name' => 'SDS C02020012 MethylCap Protein BE nl',
	'language' => 'nl',
	'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
	'countries' => 'BE',
	'modified' => '2024-11-19 18:00:25',
	'created' => '2024-11-19 18:00:25',
	'ProductsSafetySheet' => array(
		'id' => '6351',
		'product_id' => '1889',
		'safety_sheet_id' => '3859'
	)
)
$publication = array(
	'id' => '2867',
	'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
	'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
	'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
	'date' => '2015-08-05',
	'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
	'doi' => '10.1371/journal.pone.0132136',
	'modified' => '2016-03-23 09:56:34',
	'created' => '2016-03-23 09:56:34',
	'ProductsPublication' => array(
		'id' => '580',
		'product_id' => '1889',
		'publication_id' => '2867'
	)
)
$externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26244509" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap protein',
	'meta_title' => 'MethylCap protein',
	'product' => array(
		'Product' => array(
			'id' => '1889',
			'antibody_id' => null,
			'name' => 'MethylCap protein',
			'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C02020012',
			'old_catalog_number' => 'mbd-001-100',
			'sf_code' => 'C02020012-D001-000526',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '500',
			'price_USD' => '565',
			'price_GBP' => '460',
			'price_JPY' => '78325',
			'price_CNY' => '',
			'price_AUD' => '1412',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-protein-100-ug',
			'meta_title' => 'MethylCap protein',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap protein',
			'modified' => '2024-11-19 17:58:50',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'MethylCap protein'
$meta_title = 'MethylCap protein'
$product = array(
	'Product' => array(
		'id' => '1889',
		'antibody_id' => null,
		'name' => 'MethylCap protein',
		'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C02020012',
		'old_catalog_number' => 'mbd-001-100',
		'sf_code' => 'C02020012-D001-000526',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '500',
		'price_USD' => '565',
		'price_GBP' => '460',
		'price_JPY' => '78325',
		'price_CNY' => '',
		'price_AUD' => '1412',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'methylcap-protein-100-ug',
		'meta_title' => 'MethylCap protein',
		'meta_keywords' => '',
		'meta_description' => 'MethylCap protein',
		'modified' => '2024-11-19 17:58:50',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1922',
			'antibody_id' => null,
			'name' => 'XL GenDNA Extraction Module',
			'description' => '<p><span>This module has been designed for the extraction of <strong>60 &micro;g </strong>of<strong> genomic DNA</strong> from cultured cells. The protocol and reagents allow you to perform <strong>6 extractions</strong> of <strong>10 &micro;g</strong> of genomic DNA per sample, starting with&nbsp;1 to 1.5 million cells.</span></p>
<p><span>Please check our manual&nbsp;to scale&nbsp;your needs&nbsp;based on your starting material.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '6 rxns',
			'catalog_number' => 'C03030020',
			'old_catalog_number' => 'mc-magme-003',
			'sf_code' => 'C03030020-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '115',
			'price_USD' => '135',
			'price_GBP' => '105',
			'price_JPY' => '18015',
			'price_CNY' => '',
			'price_AUD' => '338',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'xl-gendna-extraction-module-6-rxns',
			'meta_title' => 'XL GenDNA Extraction Module',
			'meta_keywords' => '',
			'meta_description' => 'XL GenDNA Extraction Module',
			'modified' => '2019-04-11 14:22:10',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1819',
			'antibody_id' => null,
			'name' => 'DiaMag 0.2ml - magnetic rack',
			'description' => '<p>The DiaMag02 is a powerful magnet which has been designed for controlled and rapid isolation of your DNA bound to magnetic beads. It allows for processing 16 samples at a time.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '1 unit',
			'catalog_number' => 'B04000001',
			'old_catalog_number' => 'kch-816-001',
			'sf_code' => 'B04000001-',
			'type' => 'ACC',
			'search_order' => '04-undefined',
			'price_EUR' => '245',
			'price_USD' => '235',
			'price_GBP' => '215',
			'price_JPY' => '38380',
			'price_CNY' => '',
			'price_AUD' => '588',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'diamag02-magnetic-rack-1-unit',
			'meta_title' => 'DiaMag02 - magnetic rack',
			'meta_keywords' => '',
			'meta_description' => 'DiaMag02 - magnetic rack',
			'modified' => '2019-06-11 16:27:35',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1887',
			'antibody_id' => null,
			'name' => 'MethylCap kit',
			'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020010',
			'old_catalog_number' => 'AF-100-0048',
			'sf_code' => 'C02020010-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-kit-x48-48-rxns',
			'meta_title' => 'MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap kit x48',
			'modified' => '2024-11-21 06:38:46',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '53',
			'position' => '4',
			'parent_id' => '12',
			'name' => 'Methylbinding domain protein',
			'description' => '<p>The MBD technology used in our <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> is based on the very high affinity of&nbsp;the <a href="https://www.diagenode.com/en/p/methylcap-protein-100-ug">MethylCap protein</a>&nbsp;for methylated DNA. This protein consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag.&nbsp;</p>
<p>Diagenode&rsquo;s <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the <a href="https://www.diagenode.com/en/p/bioruptorpico2">Bioruptor&reg; sonication device</a>.</p>
<h2>How it works</h2>
<center><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/methyl_binding_domain_overview.jpg" /></center>
<h3 class="diacol">ADVANTAGES</h3>
<ul style="font-size: 19px;" class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> &amp; <strong>reproducible</strong> technique</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'methylbinding-domain-protein-assay',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Methylbinding domain protein,DNA methylation',
			'meta_description' => 'Diagenode's High affinity protein-based isolation of methylated DNA for Methylbinding domain protein assay',
			'meta_title' => 'Methylbinding domain protein assay for DNA methylation | Diagenode',
			'modified' => '2020-09-17 13:24:55',
			'created' => '2015-07-08 09:36:55',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '102',
			'name' => 'Datasheet MethylCap Protein',
			'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
			'slug' => 'datasheet-methylcap-protein',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-09-17 13:07:11',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '2867',
			'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
			'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
			'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
			'date' => '2015-08-05',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
			'doi' => '10.1371/journal.pone.0132136',
			'modified' => '2016-03-23 09:56:34',
			'created' => '2016-03-23 09:56:34',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '3865',
			'name' => 'SDS C02020012 MethylCap Protein US en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2024-11-19 18:08:07',
			'created' => '2024-11-19 18:08:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3863',
			'name' => 'SDS C02020012 MethylCap Protein GB en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2024-11-19 18:06:33',
			'created' => '2024-11-19 18:06:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '3858',
			'name' => 'SDS C02020012 MethylCap Protein BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 17:59:07',
			'created' => '2024-11-19 17:59:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3862',
			'name' => 'SDS C02020012 MethylCap Protein FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-11-19 18:05:47',
			'created' => '2024-11-19 18:05:47',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3861',
			'name' => 'SDS C02020012 MethylCap Protein ES es',
			'language' => 'es',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-11-19 18:03:27',
			'created' => '2024-11-19 18:03:27',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3860',
			'name' => 'SDS C02020012 MethylCap Protein DE de',
			'language' => 'de',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-11-19 18:02:09',
			'created' => '2024-11-19 18:01:50',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3864',
			'name' => 'SDS C02020012 MethylCap Protein JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-11-19 18:07:21',
			'created' => '2024-11-19 18:07:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3859',
			'name' => 'SDS C02020012 MethylCap Protein BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 18:00:25',
			'created' => '2024-11-19 18:00:25',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto MethylCap kit個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/xl-gendna-extraction-module-6-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03030020</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1922" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1922" id="CartAdd/1922Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1922" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>XL GenDNA Extraction Module個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                     '135',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                    '135',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="xl-gendna-extraction-module-6-rxns" data-reveal-id="cartModal-1922" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">XL GenDNA Extraction Module</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/diamag02-magnetic-rack-1-unit"><img src="/img/product/reagents/diamag02.png" alt="some alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">B04000001</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1819" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1819" id="CartAdd/1819Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1819" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>DiaMag 0.2ml - magnetic rack個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                     '235',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                    '235',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="diamag02-magnetic-rack-1-unit" data-reveal-id="cartModal-1819" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">DiaMag 0.2ml - マグネットラック</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MethylCap kit</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1887',
	'antibody_id' => null,
	'name' => 'MethylCap kit',
	'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
	'label1' => 'Characteristics',
	'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '48 rxns',
	'catalog_number' => 'C02020010',
	'old_catalog_number' => 'AF-100-0048',
	'sf_code' => 'C02020010-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '740',
	'price_USD' => '695',
	'price_GBP' => '675',
	'price_JPY' => '115920',
	'price_CNY' => '',
	'price_AUD' => '1738',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'methylcap-kit-x48-48-rxns',
	'meta_title' => 'MethylCap kit x48',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap kit x48',
	'modified' => '2024-11-21 06:38:46',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '4379',
		'product_id' => '1889',
		'related_id' => '1887'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mbd-001-100)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '102',
	'name' => 'Datasheet MethylCap Protein',
	'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
	'slug' => 'datasheet-methylcap-protein',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-09-17 13:07:11',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '713',
		'product_id' => '1889',
		'document_id' => '102'
	)
)
$sds = array(
	'id' => '3859',
	'name' => 'SDS C02020012 MethylCap Protein BE nl',
	'language' => 'nl',
	'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
	'countries' => 'BE',
	'modified' => '2024-11-19 18:00:25',
	'created' => '2024-11-19 18:00:25',
	'ProductsSafetySheet' => array(
		'id' => '6351',
		'product_id' => '1889',
		'safety_sheet_id' => '3859'
	)
)
$publication = array(
	'id' => '2867',
	'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
	'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
	'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
	'date' => '2015-08-05',
	'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
	'doi' => '10.1371/journal.pone.0132136',
	'modified' => '2016-03-23 09:56:34',
	'created' => '2016-03-23 09:56:34',
	'ProductsPublication' => array(
		'id' => '580',
		'product_id' => '1889',
		'publication_id' => '2867'
	)
)
$externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26244509" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap protein',
	'meta_title' => 'MethylCap protein',
	'product' => array(
		'Product' => array(
			'id' => '1889',
			'antibody_id' => null,
			'name' => 'MethylCap protein',
			'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C02020012',
			'old_catalog_number' => 'mbd-001-100',
			'sf_code' => 'C02020012-D001-000526',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '500',
			'price_USD' => '565',
			'price_GBP' => '460',
			'price_JPY' => '78325',
			'price_CNY' => '',
			'price_AUD' => '1412',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-protein-100-ug',
			'meta_title' => 'MethylCap protein',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap protein',
			'modified' => '2024-11-19 17:58:50',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'MethylCap protein'
$meta_title = 'MethylCap protein'
$product = array(
	'Product' => array(
		'id' => '1889',
		'antibody_id' => null,
		'name' => 'MethylCap protein',
		'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C02020012',
		'old_catalog_number' => 'mbd-001-100',
		'sf_code' => 'C02020012-D001-000526',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '500',
		'price_USD' => '565',
		'price_GBP' => '460',
		'price_JPY' => '78325',
		'price_CNY' => '',
		'price_AUD' => '1412',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'methylcap-protein-100-ug',
		'meta_title' => 'MethylCap protein',
		'meta_keywords' => '',
		'meta_description' => 'MethylCap protein',
		'modified' => '2024-11-19 17:58:50',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1922',
			'antibody_id' => null,
			'name' => 'XL GenDNA Extraction Module',
			'description' => '<p><span>This module has been designed for the extraction of <strong>60 &micro;g </strong>of<strong> genomic DNA</strong> from cultured cells. The protocol and reagents allow you to perform <strong>6 extractions</strong> of <strong>10 &micro;g</strong> of genomic DNA per sample, starting with&nbsp;1 to 1.5 million cells.</span></p>
<p><span>Please check our manual&nbsp;to scale&nbsp;your needs&nbsp;based on your starting material.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '6 rxns',
			'catalog_number' => 'C03030020',
			'old_catalog_number' => 'mc-magme-003',
			'sf_code' => 'C03030020-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '115',
			'price_USD' => '135',
			'price_GBP' => '105',
			'price_JPY' => '18015',
			'price_CNY' => '',
			'price_AUD' => '338',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'xl-gendna-extraction-module-6-rxns',
			'meta_title' => 'XL GenDNA Extraction Module',
			'meta_keywords' => '',
			'meta_description' => 'XL GenDNA Extraction Module',
			'modified' => '2019-04-11 14:22:10',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1819',
			'antibody_id' => null,
			'name' => 'DiaMag 0.2ml - magnetic rack',
			'description' => '<p>The DiaMag02 is a powerful magnet which has been designed for controlled and rapid isolation of your DNA bound to magnetic beads. It allows for processing 16 samples at a time.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '1 unit',
			'catalog_number' => 'B04000001',
			'old_catalog_number' => 'kch-816-001',
			'sf_code' => 'B04000001-',
			'type' => 'ACC',
			'search_order' => '04-undefined',
			'price_EUR' => '245',
			'price_USD' => '235',
			'price_GBP' => '215',
			'price_JPY' => '38380',
			'price_CNY' => '',
			'price_AUD' => '588',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'diamag02-magnetic-rack-1-unit',
			'meta_title' => 'DiaMag02 - magnetic rack',
			'meta_keywords' => '',
			'meta_description' => 'DiaMag02 - magnetic rack',
			'modified' => '2019-06-11 16:27:35',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1887',
			'antibody_id' => null,
			'name' => 'MethylCap kit',
			'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020010',
			'old_catalog_number' => 'AF-100-0048',
			'sf_code' => 'C02020010-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-kit-x48-48-rxns',
			'meta_title' => 'MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap kit x48',
			'modified' => '2024-11-21 06:38:46',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '53',
			'position' => '4',
			'parent_id' => '12',
			'name' => 'Methylbinding domain protein',
			'description' => '<p>The MBD technology used in our <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> is based on the very high affinity of&nbsp;the <a href="https://www.diagenode.com/en/p/methylcap-protein-100-ug">MethylCap protein</a>&nbsp;for methylated DNA. This protein consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag.&nbsp;</p>
<p>Diagenode&rsquo;s <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the <a href="https://www.diagenode.com/en/p/bioruptorpico2">Bioruptor&reg; sonication device</a>.</p>
<h2>How it works</h2>
<center><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/methyl_binding_domain_overview.jpg" /></center>
<h3 class="diacol">ADVANTAGES</h3>
<ul style="font-size: 19px;" class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> &amp; <strong>reproducible</strong> technique</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'methylbinding-domain-protein-assay',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Methylbinding domain protein,DNA methylation',
			'meta_description' => 'Diagenode's High affinity protein-based isolation of methylated DNA for Methylbinding domain protein assay',
			'meta_title' => 'Methylbinding domain protein assay for DNA methylation | Diagenode',
			'modified' => '2020-09-17 13:24:55',
			'created' => '2015-07-08 09:36:55',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '102',
			'name' => 'Datasheet MethylCap Protein',
			'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
			'slug' => 'datasheet-methylcap-protein',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-09-17 13:07:11',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '2867',
			'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
			'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
			'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
			'date' => '2015-08-05',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
			'doi' => '10.1371/journal.pone.0132136',
			'modified' => '2016-03-23 09:56:34',
			'created' => '2016-03-23 09:56:34',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '3865',
			'name' => 'SDS C02020012 MethylCap Protein US en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2024-11-19 18:08:07',
			'created' => '2024-11-19 18:08:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3863',
			'name' => 'SDS C02020012 MethylCap Protein GB en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2024-11-19 18:06:33',
			'created' => '2024-11-19 18:06:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '3858',
			'name' => 'SDS C02020012 MethylCap Protein BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 17:59:07',
			'created' => '2024-11-19 17:59:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3862',
			'name' => 'SDS C02020012 MethylCap Protein FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-11-19 18:05:47',
			'created' => '2024-11-19 18:05:47',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3861',
			'name' => 'SDS C02020012 MethylCap Protein ES es',
			'language' => 'es',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-11-19 18:03:27',
			'created' => '2024-11-19 18:03:27',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3860',
			'name' => 'SDS C02020012 MethylCap Protein DE de',
			'language' => 'de',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-11-19 18:02:09',
			'created' => '2024-11-19 18:01:50',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3864',
			'name' => 'SDS C02020012 MethylCap Protein JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-11-19 18:07:21',
			'created' => '2024-11-19 18:07:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3859',
			'name' => 'SDS C02020012 MethylCap Protein BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 18:00:25',
			'created' => '2024-11-19 18:00:25',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto MethylCap kit個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/xl-gendna-extraction-module-6-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03030020</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1922" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1922" id="CartAdd/1922Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1922" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>XL GenDNA Extraction Module個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                     '135',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                    '135',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="xl-gendna-extraction-module-6-rxns" data-reveal-id="cartModal-1922" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">XL GenDNA Extraction Module</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/diamag02-magnetic-rack-1-unit"><img src="/img/product/reagents/diamag02.png" alt="some alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">B04000001</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1819" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1819" id="CartAdd/1819Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1819" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>DiaMag 0.2ml - magnetic rack個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                     '235',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                    '235',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="diamag02-magnetic-rack-1-unit" data-reveal-id="cartModal-1819" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">DiaMag 0.2ml - マグネットラック</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MethylCap kit</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1887',
	'antibody_id' => null,
	'name' => 'MethylCap kit',
	'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
	'label1' => 'Characteristics',
	'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '48 rxns',
	'catalog_number' => 'C02020010',
	'old_catalog_number' => 'AF-100-0048',
	'sf_code' => 'C02020010-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '740',
	'price_USD' => '695',
	'price_GBP' => '675',
	'price_JPY' => '115920',
	'price_CNY' => '',
	'price_AUD' => '1738',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'methylcap-kit-x48-48-rxns',
	'meta_title' => 'MethylCap kit x48',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap kit x48',
	'modified' => '2024-11-21 06:38:46',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '4379',
		'product_id' => '1889',
		'related_id' => '1887'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mbd-001-100)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '102',
	'name' => 'Datasheet MethylCap Protein',
	'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
	'slug' => 'datasheet-methylcap-protein',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-09-17 13:07:11',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '713',
		'product_id' => '1889',
		'document_id' => '102'
	)
)
$sds = array(
	'id' => '3859',
	'name' => 'SDS C02020012 MethylCap Protein BE nl',
	'language' => 'nl',
	'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
	'countries' => 'BE',
	'modified' => '2024-11-19 18:00:25',
	'created' => '2024-11-19 18:00:25',
	'ProductsSafetySheet' => array(
		'id' => '6351',
		'product_id' => '1889',
		'safety_sheet_id' => '3859'
	)
)
$publication = array(
	'id' => '2867',
	'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
	'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
	'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
	'date' => '2015-08-05',
	'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
	'doi' => '10.1371/journal.pone.0132136',
	'modified' => '2016-03-23 09:56:34',
	'created' => '2016-03-23 09:56:34',
	'ProductsPublication' => array(
		'id' => '580',
		'product_id' => '1889',
		'publication_id' => '2867'
	)
)
$externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26244509" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap protein',
	'meta_title' => 'MethylCap protein',
	'product' => array(
		'Product' => array(
			'id' => '1889',
			'antibody_id' => null,
			'name' => 'MethylCap protein',
			'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µg',
			'catalog_number' => 'C02020012',
			'old_catalog_number' => 'mbd-001-100',
			'sf_code' => 'C02020012-D001-000526',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '500',
			'price_USD' => '565',
			'price_GBP' => '460',
			'price_JPY' => '78325',
			'price_CNY' => '',
			'price_AUD' => '1412',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-protein-100-ug',
			'meta_title' => 'MethylCap protein',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap protein',
			'modified' => '2024-11-19 17:58:50',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'MethylCap protein'
$meta_title = 'MethylCap protein'
$product = array(
	'Product' => array(
		'id' => '1889',
		'antibody_id' => null,
		'name' => 'MethylCap protein',
		'description' => '<p>メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することができる細胞の数です。</p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '100 µg',
		'catalog_number' => 'C02020012',
		'old_catalog_number' => 'mbd-001-100',
		'sf_code' => 'C02020012-D001-000526',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '500',
		'price_USD' => '565',
		'price_GBP' => '460',
		'price_JPY' => '78325',
		'price_CNY' => '',
		'price_AUD' => '1412',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'methylcap-protein-100-ug',
		'meta_title' => 'MethylCap protein',
		'meta_keywords' => '',
		'meta_description' => 'MethylCap protein',
		'modified' => '2024-11-19 17:58:50',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1888',
			'antibody_id' => null,
			'name' => 'Auto MethylCap kit',
			'description' => '<p>The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. This procedure has been optimized to perform automated immunoprecipitation of chromatin using the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a> enabling highly reproducible results and allowing for high throughput.</p>',
			'label1' => ' Characteristics',
			'info1' => '<ul>
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>Automation compatibility</strong><strong></strong>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>F</strong><strong>igure 1.</strong><span>&nbsp;</span>Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).<br /><strong></strong></p>
</li>
</ul>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020011',
			'old_catalog_number' => 'AF-Auto01-0048',
			'sf_code' => 'C02020011-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-methylcap-kit-x48-48-rxns',
			'meta_title' => 'Auto MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'Auto MethylCap kit x48',
			'modified' => '2020-09-17 13:33:12',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1922',
			'antibody_id' => null,
			'name' => 'XL GenDNA Extraction Module',
			'description' => '<p><span>This module has been designed for the extraction of <strong>60 &micro;g </strong>of<strong> genomic DNA</strong> from cultured cells. The protocol and reagents allow you to perform <strong>6 extractions</strong> of <strong>10 &micro;g</strong> of genomic DNA per sample, starting with&nbsp;1 to 1.5 million cells.</span></p>
<p><span>Please check our manual&nbsp;to scale&nbsp;your needs&nbsp;based on your starting material.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '6 rxns',
			'catalog_number' => 'C03030020',
			'old_catalog_number' => 'mc-magme-003',
			'sf_code' => 'C03030020-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '115',
			'price_USD' => '135',
			'price_GBP' => '105',
			'price_JPY' => '18015',
			'price_CNY' => '',
			'price_AUD' => '338',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'xl-gendna-extraction-module-6-rxns',
			'meta_title' => 'XL GenDNA Extraction Module',
			'meta_keywords' => '',
			'meta_description' => 'XL GenDNA Extraction Module',
			'modified' => '2019-04-11 14:22:10',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1819',
			'antibody_id' => null,
			'name' => 'DiaMag 0.2ml - magnetic rack',
			'description' => '<p>The DiaMag02 is a powerful magnet which has been designed for controlled and rapid isolation of your DNA bound to magnetic beads. It allows for processing 16 samples at a time.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '1 unit',
			'catalog_number' => 'B04000001',
			'old_catalog_number' => 'kch-816-001',
			'sf_code' => 'B04000001-',
			'type' => 'ACC',
			'search_order' => '04-undefined',
			'price_EUR' => '245',
			'price_USD' => '235',
			'price_GBP' => '215',
			'price_JPY' => '38380',
			'price_CNY' => '',
			'price_AUD' => '588',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'diamag02-magnetic-rack-1-unit',
			'meta_title' => 'DiaMag02 - magnetic rack',
			'meta_keywords' => '',
			'meta_description' => 'DiaMag02 - magnetic rack',
			'modified' => '2019-06-11 16:27:35',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1887',
			'antibody_id' => null,
			'name' => 'MethylCap kit',
			'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C02020010',
			'old_catalog_number' => 'AF-100-0048',
			'sf_code' => 'C02020010-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '695',
			'price_GBP' => '675',
			'price_JPY' => '115920',
			'price_CNY' => '',
			'price_AUD' => '1738',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'methylcap-kit-x48-48-rxns',
			'meta_title' => 'MethylCap kit x48',
			'meta_keywords' => '',
			'meta_description' => 'MethylCap kit x48',
			'modified' => '2024-11-21 06:38:46',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '53',
			'position' => '4',
			'parent_id' => '12',
			'name' => 'Methylbinding domain protein',
			'description' => '<p>The MBD technology used in our <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> is based on the very high affinity of&nbsp;the <a href="https://www.diagenode.com/en/p/methylcap-protein-100-ug">MethylCap protein</a>&nbsp;for methylated DNA. This protein consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag.&nbsp;</p>
<p>Diagenode&rsquo;s <a href="https://www.diagenode.com/en/p/methylcap-kit-x48-48-rxns">MethylCap kit</a> enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the <a href="https://www.diagenode.com/en/p/bioruptorpico2">Bioruptor&reg; sonication device</a>.</p>
<h2>How it works</h2>
<center><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/methyl_binding_domain_overview.jpg" /></center>
<h3 class="diacol">ADVANTAGES</h3>
<ul style="font-size: 19px;" class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> &amp; <strong>reproducible</strong> technique</li>
<li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'methylbinding-domain-protein-assay',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Methylbinding domain protein,DNA methylation',
			'meta_description' => 'Diagenode's High affinity protein-based isolation of methylated DNA for Methylbinding domain protein assay',
			'meta_title' => 'Methylbinding domain protein assay for DNA methylation | Diagenode',
			'modified' => '2020-09-17 13:24:55',
			'created' => '2015-07-08 09:36:55',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '102',
			'name' => 'Datasheet MethylCap Protein',
			'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
			'slug' => 'datasheet-methylcap-protein',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2020-09-17 13:07:11',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '2867',
			'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
			'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
			'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
			'date' => '2015-08-05',
			'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
			'doi' => '10.1371/journal.pone.0132136',
			'modified' => '2016-03-23 09:56:34',
			'created' => '2016-03-23 09:56:34',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '3865',
			'name' => 'SDS C02020012 MethylCap Protein US en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2024-11-19 18:08:07',
			'created' => '2024-11-19 18:08:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3863',
			'name' => 'SDS C02020012 MethylCap Protein GB en',
			'language' => 'en',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2024-11-19 18:06:33',
			'created' => '2024-11-19 18:06:33',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '3858',
			'name' => 'SDS C02020012 MethylCap Protein BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 17:59:07',
			'created' => '2024-11-19 17:59:07',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3862',
			'name' => 'SDS C02020012 MethylCap Protein FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-11-19 18:05:47',
			'created' => '2024-11-19 18:05:47',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3861',
			'name' => 'SDS C02020012 MethylCap Protein ES es',
			'language' => 'es',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-11-19 18:03:27',
			'created' => '2024-11-19 18:03:27',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3860',
			'name' => 'SDS C02020012 MethylCap Protein DE de',
			'language' => 'de',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-11-19 18:02:09',
			'created' => '2024-11-19 18:01:50',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3864',
			'name' => 'SDS C02020012 MethylCap Protein JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-11-19 18:07:21',
			'created' => '2024-11-19 18:07:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3859',
			'name' => 'SDS C02020012 MethylCap Protein BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-11-19 18:00:25',
			'created' => '2024-11-19 18:00:25',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/auto-methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020011</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1888" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1888" id="CartAdd/1888Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1888" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto MethylCap kit個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                     '695',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto MethylCap kit',
                    'C02020011',
                    '695',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-methylcap-kit-x48-48-rxns" data-reveal-id="cartModal-1888" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto MethylCap kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/xl-gendna-extraction-module-6-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03030020</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1922" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1922" id="CartAdd/1922Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1922" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>XL GenDNA Extraction Module個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                     '135',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('XL GenDNA Extraction Module',
                    'C03030020',
                    '135',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="xl-gendna-extraction-module-6-rxns" data-reveal-id="cartModal-1922" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">XL GenDNA Extraction Module</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/diamag02-magnetic-rack-1-unit"><img src="/img/product/reagents/diamag02.png" alt="some alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">B04000001</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1819" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1819" id="CartAdd/1819Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1819" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>DiaMag 0.2ml - magnetic rack個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                     '235',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('DiaMag 0.2ml - magnetic rack',
                    'B04000001',
                    '235',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="diamag02-magnetic-rack-1-unit" data-reveal-id="cartModal-1819" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">DiaMag 0.2ml - マグネットラック</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/methylcap-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02020010</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MethylCap kit</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '1887',
	'antibody_id' => null,
	'name' => 'MethylCap kit',
	'description' => '<p>The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA using methyl-CpG-binding domain (MBD) of human MeCP2 protein. The procedure has been adapted to both manual process or <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star&reg; Compact Automated System</a>. Libraries of captured methylated DNA can be prepared for next-generation sequencing (NGS) by combining MBD technology with the <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kit v3</a>.</p>',
	'label1' => 'Characteristics',
	'info1' => '<ul style="list-style-type: circle;">
<li><strong>Fast &amp; sensitive capture</strong> of methylated DNA</li>
<li><strong>High capture efficiency</strong></li>
<li><strong>Differential fractionation</strong> of methylated DNA by CpG density (3 eluted fractions)</li>
<li><strong>On-day protocol</strong></li>
<li><strong>NGS compatibility</strong></li>
</ul>
<h3>MBD-seq allows for detection of genomic regions with different CpG density</h3>
<p><img src="https://www.diagenode.com/img/product/kits/mbd_results1.png" alt="MBD-sequencing results have been validated by bisulfite sequencing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong></strong></p>
<p><strong></strong><strong>F</strong><strong>igure 1.</strong> Using the MBD approach, two methylated regions were detected in different elution fractions according to their methylated CpG density (A). Low, Medium and High refer to the sequenced DNA from different elution fractions with increasing salt concentration. Methylated patterns of these two different methylated regions were validated by bisulfite conversion assay (B).</p>',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '48 rxns',
	'catalog_number' => 'C02020010',
	'old_catalog_number' => 'AF-100-0048',
	'sf_code' => 'C02020010-',
	'type' => 'RFR',
	'search_order' => '04-undefined',
	'price_EUR' => '740',
	'price_USD' => '695',
	'price_GBP' => '675',
	'price_JPY' => '115920',
	'price_CNY' => '',
	'price_AUD' => '1738',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => true,
	'featured' => true,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'methylcap-kit-x48-48-rxns',
	'meta_title' => 'MethylCap kit x48',
	'meta_keywords' => '',
	'meta_description' => 'MethylCap kit x48',
	'modified' => '2024-11-21 06:38:46',
	'created' => '2015-06-29 14:08:20',
	'ProductsRelated' => array(
		'id' => '4379',
		'product_id' => '1889',
		'related_id' => '1887'
	),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	)
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mbd-001-100)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '102',
	'name' => 'Datasheet MethylCap Protein',
	'description' => '<p>The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. A single fully methylated CpG is sufficient for the interaction between the MethylCap protein and methylated DNA fragments.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/kits/Datasheet_MethylCap_Protein.pdf',
	'slug' => 'datasheet-methylcap-protein',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2020-09-17 13:07:11',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '713',
		'product_id' => '1889',
		'document_id' => '102'
	)
)
$sds = array(
	'id' => '3859',
	'name' => 'SDS C02020012 MethylCap Protein BE nl',
	'language' => 'nl',
	'url' => 'files/SDS/MethylCap_Protein/SDS-C02020012-MethylCap_protein-BE-nl-GHS_2_0.pdf',
	'countries' => 'BE',
	'modified' => '2024-11-19 18:00:25',
	'created' => '2024-11-19 18:00:25',
	'ProductsSafetySheet' => array(
		'id' => '6351',
		'product_id' => '1889',
		'safety_sheet_id' => '3859'
	)
)
$publication = array(
	'id' => '2867',
	'name' => 'Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.',
	'authors' => 'Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A',
	'description' => '<p>Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.</p>',
	'date' => '2015-08-05',
	'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26244509',
	'doi' => '10.1371/journal.pone.0132136',
	'modified' => '2016-03-23 09:56:34',
	'created' => '2016-03-23 09:56:34',
	'ProductsPublication' => array(
		'id' => '580',
		'product_id' => '1889',
		'publication_id' => '2867'
	)
)
$externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26244509" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Contact Information

First name

Last name

Company

Phone number

City

Country

State

Email verification^*

Comment

イベント

すべてのイベントを見る

Site map | Contact us | Conditions of sales | Conditions of purchase | Privacy policy

SDS C02020012 MethylCap Protein US en	Download
SDS C02020012 MethylCap Protein GB en	Download
SDS C02020012 MethylCap Protein BE fr	Download
SDS C02020012 MethylCap Protein FR fr	Download
SDS C02020012 MethylCap Protein ES es	Download
SDS C02020012 MethylCap Protein DE de	Download
SDS C02020012 MethylCap Protein JP ja	Download
SDS C02020012 MethylCap Protein BE nl	Download