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TASP1 polyclonal antibody

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Catalog Number

Format

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C15310115
(CS-115-100)

100 µl

$260.00
Bulk order

Alternative name: Taspase1

Polyclonal antibody raised in rabbit against mouse human TASP1 (Threonine aspartase 1), using three KLH-conjugated synthetic peptides: two containing an amino acid sequence from the N-terminal part of the protein and one containing an amino acid sequence from the central part of the protein.

Lot	A175-001
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:1,000	Fig 1
Western Blotting	1:500	Fig 2

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500.

Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.

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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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<p><span>Polyclonal antibody raised in rabbit against mouse human TASP1 (Threonine aspartase 1), using three KLH-conjugated synthetic peptides: two containing an amino acid sequence from the N-terminal part of the protein and one containing an amino acid sequence from the central part of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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<div class="row">
<div class="small-3 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310115_fig2.jpg" alt="Western blot" height="130" width="109" /></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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<div class="small-3 columns">
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Datasheet TASP1 CS-115-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

TASP1 polyclonal antibody

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