Alternative name: Taspase1
Polyclonal antibody raised in rabbit against mouse human TASP1 (Threonine aspartase 1), using three KLH-conjugated synthetic peptides: two containing an amino acid sequence from the N-terminal part of the protein and one containing an amino acid sequence from the central part of the protein.
| Lot | A175-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:1,000 | Fig 1 |
| Western Blotting | 1:500 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500.
Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.
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