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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human TASP1 (Cat. No. CS-115-100). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:43,500. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against TASP1</strong><br /> Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against TASP1 (Cat. No. CS-115-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 44.5 kDa) is indicated on the left; the marker (in kDa) is shown on the right.</small></p>
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Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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