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Chromatin Analysis

ChIP-seq

Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.
The ChIP-seq workflow


  1. Chromatin preparation: Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.
  2. Chromatin shearing: Fragment chromatin by sonication to desired fragment size (100-500 bp)
  3. Chromatin IP: Capture protein-DNA complexes with specific ChIP-seq grade antibodies against the histone or transcription factor of interest
  4. DNA purification: Reverse cross-links, elute, and purify 
  5. NGS Library Preparation: Ligate adapters and amplify IP'd material
  6. Bioinformatic analysis: Perform read filtering and trimming, read specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc.

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

针对 ChIP-seq 的推荐产品

Cat. No.ProductFormatPrice
C01010171 Auto iDeal ChIP-seq kit for Histones
This product must be used with the IP-Star Compact Automated System. Don’t risk wasting your precious sequencing samples. Diagenode’s validated&... 		100 rxns $2,340.00
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors
This product must be used with the IP-Star Compact Automated System. Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly sp... 		100 rxns $2,340.00
C01010153 Auto Universal Plant ChIP-seq kit
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma... 		24 rxns $575.00
B01080010 Bioruptor® Pico 非接触式超声波破碎仪
自 2004 年以来，Diagenode 不断积累细胞、核酸等生物样本剪切专业知识，用于设计 Bioruptor® Pico 并确保提供最佳的样本制备体验，使其广泛应用于各种研究领域（包括环境研究、毒理学、基因组学和表观基因组学、癌症研究、干细胞和发育、神经科学、临床应用、农业等）。 Bioruptor... 		1 unit
C01019027 ChIP Cross-link Gold
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective in cr... 		600 µl $160.00
C01010051 iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit for Histones has everything you need for a successf... 		4 chrom. prep./24 IPs $1,130.00
C01010055 iDeal ChIP-seq kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly validated solution for robust transcription factor and other non-histone proteins ... 		4 chrom. prep./24 IPs $1,130.00
C01010132 True MicroChIP Kit
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for chromatin preparation for downstream ChIP-seq and ChIP-qPC... 		20 rxns $680.00
C01010152 Universal Plant ChIP-seq kit
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, ... 		24 rxns $575.00
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker). 		24 rxns $280.00
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System. 		100 pc $45.00
C30080030 96 well microplates for IP-Star®
Standard 96 well transparent plates made by polyestyrene and compatible with automated systems. Solid U-bottom plates free of DNAase and RNAase and human DNA. 		10 pc $85.00
C01011000 Auto ChIPmentation Kit for Histones
Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ... 		24 rxns
C01010058 Auto iDeal ChIP-seq Kit for Transcription Factors
This product must be used with the IP-Star Compact Automated System. Diagenode’s Auto iDeal ChIP-seq Kit for Transcription Factors is a highly sp... 		24 rxns $1,130.00
C03010010 Auto IPure kit v2
This product must be used with the IP-Star Compact Automated System. The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applicat... 		100 rxns $410.00
C02020011 Auto MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa... 		48 rxns $695.00
C15410337 BRD4 Antibody - ChIP-seq Grade
Polyclonal antibody raised in rabbit against human BRD4 (Bromodomain Containing 4), using two KLH-conjugated synthetic peptides from the N-terminal and centr... 		50 µg $380.00
C01011010 ChIPmentation Kit for Histones
Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ... 		24 rxns
C15210021 H2A.Z monoclonal antibody
Polyclonal antibody raised in rabbit against histone variant H2A.Z, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part o... 		100 µg $440.00
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker). 		24 rxns $280.00
C03010015 IPure kit v2
High DNA recovery after ChIP or MeDIP is critical for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only D... 		100 rxns $445.00
C17040004-50 Maize B73 inner stem ZmB1-UTR primer pair
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste... 		50 µl $35.00
C17040005-50 Maize B73 inner stem ZmCopia primer pair
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ... 		50 µl $35.00
C17040011-50 Poplar xylem PtrCopia-orth primer pair
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually... 		50 µl $55.00
C17040010-50 Poplar xylem PtrMYBTF1 primer pair
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p... 		50 µl $55.00
C17040009-50 Rice seedlings OsMADS6 primer pair
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly... 		50 µl $35.00
C15410368 SUV39H1 polyclonal antibody
Other names: KMT1A, MG44Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptid... 		50 µg $380.00
C17040006-50 Tomato leaves SlChr2-reg8 primer pair
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ... 		50 µl $35.00
C17040007-50 Tomato leaves SlChr4-NC1 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ... 		50 µl $55.00

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