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剪切技术

卓越的剪切和超声处理生物样本, 适用于任何研究领域 Diagenode致力于最先进的高质量生物和化学样本制备, 开发了业界最先进的水浴型超声仪和流体动力学装置。我们的仪器跨足了环境研究、毒理学、基因组学和表观基因组学、癌症研究、干细胞和发育、神经科学、临床应用、农业等多个研究领域。我们提供三种类型的超声/剪切装置，能确切满足您的应用需求： Bioruptor® 采用可高度可控的超声波能量及 ACT（自适式空化技术），这种非接触式能同时及恒温处理多个样本的技术，可实现剪切核酸片段大小到150 bp - 1 kb 之间，是染色质免疫沉淀 (ChIP) 染色质剪切、二代测序基因组 DNA和 RNA 剪切实验的最佳选择。同时，Bioruptor 也适用于细胞和组织破碎、质谱蛋白处理等实验。Bioruptor 有两种型号：Bioruptor Plus专门设计用于质谱蛋白处理、大体积细胞和组织样本剪切、ChIP-qPCR 及其他应用，而 Bioruptor Pico 适用于 ChIP-seq 和 NGS。 One® 是台独特的桌上型超声剪切装置，是处理少量样本, 小体积二代测序DNA 剪切（150bp - 1kb 范围内）的最佳选择。 Megaruptor® 是台能剪切 3 kb - 100 kb 核酸, 为目前片段分布最集中的长片段DNA流体动力学剪切装置，适用领域为长片段三代测序应用。 ... Learn more

Bioruptor^®

Bioruptor 超声破碎仪，采用自适应空化技术，具有最高剪切性能 Diagenode Bioruptor 是最先进的超声处理系统，在超过 4,000 篇出版物中受到好评。Bioruptor 可同时处理多达 16 份样品，可灵活选择 0.1 mL 至 50 mL 的管，从而准确实现不同片段范围的随机和无偏剪切。封闭管形式可防止样品交叉污染，从而实现无与伦比的重现性。Bioruptor 使用高度受控的超声波能量及 ACT（自适应空化技术，一种非接触式、并行处理和等温技术），是用于染色质免疫沉淀 (ChIP) 的染色质剪切、用于下一代测序的基因组 DNA 剪切、RNA 剪切、细胞和组织破碎等的理想选择。转到 Bi... Learn more

Megaruptor^®

您的长读测序合作伙伴测序技术已彻底改变了基因组学和生物学研究。长读测序使研究人员能够以更高的准确度获得更全面的基因组视图。然而，最佳基因库制备的最关键方面之一是待测序的 DNA 的质量。首先，必须将 DNA 有效且一致地剪切成适当的片段大小。Megaruptor 具备最先进的剪切性能，可使用 PacBio® 和 Oxford NanoporeTM 技术提供最佳长读测序。推荐人：PacBio 点击此处了解我们的最新创新 Learn more

One^®

The One is the desktop solution for DNA shearing. Learn more

Maintenance Plans

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Automation

Sales of the IP-Star Compact instrument have been discontinued. Supporting materials, repairs, and consumables will still be available Supporting Materials Documents Antibody ChIPSeq QC using the IPStar Compact POSTERChromatin immunoprecipitation (ChIP) is the most widely used method to study protein-DNA interact... Download High Resolution ChIPSeq Profiles with IPStar Automated Platform POSTERChromatin immunoprecipitation followed by next generation sequencing (ChIP-Seq) is a powerful tec... Download Automated method for RNAseq libraries APPLICATION NOTEAn automated method for efficient, accura... Learn more

IP-Star accessories

The plastics have been selected and validated together with the IP-Star Compact® automated system for an optimal performance. Learn more

Chromatin studies

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci. ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites. The ChIP workflow Read more Step by step workflow Optimal so... Learn more

Chromatin shearing

The first step of ChIP - chromatin preparation - plays a key role in a succesful ChIP experiment. Each step of chromatin preparation (starting amount of material, cross-linking, cell lysis and chromatin shearing) has to be optimized, leading to a chromatin sample suitable for ChIP. A high quality chromatin sample ... Learn more

Chromatin immunoprecipitation

Diagenode offers a wide range of kits for chromatin immunoprecipitation, a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. ChIP offers insight into the regulation of gene expression. This technique is now used in a variety of life science disciplines inc... Learn more

ChIPmentation™

ChIPmentation for high-quality and fast ChIP-seq library preparation Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that ... Learn more

CUT&Tag

CUT&Tag (Cleavage Under Targets and Tagmentation) is a technique for efficient chromatin profiling described in Kaya-Okur HS et al., Nature Commun. 2019. In CUT&Tag, proteins are first bound to chromatin by a specific antibody. This complex is then fused to a protein A-Tn5 transposase, loaded with sequenci... Learn more

ATAC-seq

ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the transposase Tn5 which cuts exposed open chromatin and simultaneously ligates adapters for subsequent amplificati... Learn more

DNA methylation

Understanding DNA methylation is critical for learning about control of transcription, genomic imprinting, embryonic development, and carcinogenesis. Diagenode proposes two main approaches to studying DNA methylation - bisulfite conversion with downstream sequencing or qPCR and immunoprecipitation (IP). Diagenode Product Solutions Study methylation with single base-resolution Does sequencing cost play a role? NO YES Premium WGBS Kit Premium RRBS Kit Generate bisulfite converted gDNA libraries ready for gDNA single or paired-end sequencing of the whole genome Generate bisulfite co... Learn more

Bisulfite conversion

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine... Learn more

Methylated DNA Immunoprecipitation

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prep... Learn more

Methylbinding domain protein

The MBD technology used in our MethylCap Kit is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fus... Learn more

DNA methylation standard

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PCR enzymes and nucleotides

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Hydroxymethylated DNA Immunoprecipitation

The hydroxymethylated DNA IP (hMeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-hmC). How it works In brief, hydroxymethyl DNA IP is performed as follows: starting from sheared genomic DNA from cultured cells or tissues, the immunoselect... Learn more

Semi-automation

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Cell culture

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Genome editing (CRISPR/Cas9)

CRISPR/Cas9 The CRISPR/Cas genome editing system uses a RNA-guided endonuclease to introduce site-directed double-strand breaks (DSBs) in DNA. The endonuclease Cas9 can be used for DNA cleavage at specific sites defined by guide RNAs and has been adopted as a technology of choice for genome engineering. Cas9-mediated cleavage relies on the presence of a protospacer adjacent motif (PAM) in the target DNA. The Non Homologous End Joining (NHEJ) DNA repair pathway plays a role in repairing Cas9-induced DSBs. As the repair is prone to errors, insertions and deletions (indels) may occur that subsequently disrupt gene function. DSBs m... Learn more

Selection of guide RNA by ChIP

While a number of computational tools help design sgRNAs to target specific loci, the accurate prediction of whether Cas9 and the sgRNA of interest will bind specifically to the genome is still a challenge. In order to ensure specific targeting of CRISPR/Cas9, the verification of the specific binding of the sgRNA a... Learn more

Kits

Diagenode’s epigenetic reagents include: DNA methylation kits and antibodies - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3. ChIP and ChIP-seq kits for industry-leading specificity and sensitivity - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification r... Learn more

Antibodies

Since 2004 Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We understand that high quality is crucial for the success of your ChIP and ChIP-seq experiments, so we emphasize strict quality standards and actual validation for all of our antibodies. Our partnerships with leading epigenetics experts and epigenetics consortiums such as BLUEPRINT have allowed us to validate our antibodies to the highest degree, guaranteeing specificity, reproducibility, and success for each and every ChIP assay. In addition, unlike competing suppliers, we are fully transparent with our validation and provide all batch-s... Learn more

All antibodies

All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (requires less antibody per reaction) Batch-specific data is av... Learn more

Sample size antibodies

Validated epigenetics antibodies – care for a sample? Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-spe... Learn more

ChIP-seq grade antibodies

Unparalleled ChIP-Seq results with the most rigorously validated antibodies Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, ... Learn more

ChIP-grade antibodies

Chromatin immunoprecipitation (ChIP) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies us... Learn more

Western Blot Antibodies

We have what you need for a successful Western blot!Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly.For a successful Western blot we offer:Loading c... Learn more

Chromatin associated proteins

Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chro... Learn more

Histone modifying enzymes

Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (re... Learn more

Isotype controls

Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse. These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively. Diagenode’s highly validated antibodies: H... Learn more

DNA modifications

The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylat... Learn more

RNA associated proteins

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RNA modifications

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Histone antibodies

Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are l... Learn more

CRISPR/Cas9 antibodies

The CRISPR/Cas genome editing system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or ... Learn more

CUT&Tag Antibodies

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Transcription

The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets. Diagenode’s highly validated antibodies: Highly sen... Learn more

Epitope tags

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Other antibodies

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NGS Library preparation

Diagenode provides a complete portfolio of library preparation kits for Next-Generation Sequencing, covering RNA-seq, ChIP-seq, and DNA sequencing. Our library preparation solutions can be used for low inputs, from as little as 10 pg of starting material. Our kits offer simple and fast workflows, high yields, and ready-to-sequence DNA. Our solutions incorporate user-friendly protocols that even beginners can easily master upon the first use. Learn more

Library preparation for ChIP-seq

Library preparation following ChIP can be challenging due to the limited amount of DNA recovered after ChIP. Diagenode has developed the optimal solutions for ChIP-seq using two different approaches: the ligation-based library preparation on purified DNA or the tagmentation-based ChIPmentation. ... Learn more

Library preparation for RNA sequencing

Diagenode technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method to produce DNA libraries for next generation sequencing from low input RNA. This solution is designed for an advanced researcher who has extensive experience with RNA sequencing. While simp... Learn more

Library preparation for DNA sequencing

Diagenode’s new DNA library preparation utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of DNA. Learn more

Epigenomics Profiling Services

Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis. Chromatin analysis ChIP-seq analysis Histone modification Promoter analysis Enhancer analysis ChIP-seq analysis Transcription factor (sc)ATAC-s... Learn more

Real time PCR master mix

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Reagents

Diagenode offers full and validated solutions for every step of your epigenetics sample preparation, making epigenetics research easy, accessible, and reliable. Beside our complete kit solutions please find here below an exhaustive list of all high quality reagents realated to your epigenetics research. Learn more

Beads, magnetic racks and accessories

DiaMag beads and magnetic racks are intended for isolation of immune complexes in ChIP experiments or MeDIP experiments and have been extensively validated for these assays. The beads are compatible for immunoprecipitation of a number of IgGs and polyclonal antibodies across a number of species. Learn more

DNA/RNA purification

Diagenode offers a variety of optimized DNA purification methods allowing for recovery of high quality DNA after chromatin immunoprecipitation or methylated DNA immunoprecipitation, essential for ideal results in downstream applications such as qPCR or next generation sequencing. Our purification technology in o... Learn more

Fixation reagents

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Enzymes

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Inhibitors

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Plasmids

Our yeast low copy BirA expression plasmids are designed for in vivo yeast biotinylation systems for sensitive detection of protein-DNA complexes using chromatin immunoprecipitaion. The constructs include a nuclear localisation signal. We also offer biotinylation tagging cassettes for C- and N- terminal tagging. Learn more

Primer indexes for tagmented libraries

The Primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by tagmentation. Each primer index is baring a unique index in order to identify each library before pooling them for sequencing in the same lane. They are compatible with an... Learn more

Primer pairs

Our primer pairs have been carefully designed for high specificity to specific genomic regions. Primers have been tested and optimized to be used in a number of applications including DNA amplification after ChIP, ChIP-seq library validation, qPCR, and RT-qPCR. Learn more

Protein Extraction

Diagenode offers optimized protein extraction kits for use with the Bioruptor® sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor®. Learn more

Proteins

Diagenode now offers a broad range of tools to study nucleosomes and histones, the structural and functional units of chromatin. We provide solutions for any architectural l... Learn more

Exosomes

The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that exosomes, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including ... Learn more

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