Transposome Assembly Using Diagenode pA-Tn5 Transposase
pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.
