ChIP-seq from human tumor tissue

Application of the Bioruptor^® Pico for shearing chromatin fragments for ChIP-seq from human tumor tissue.

Over the last several years, chromatin immunoprecipitation followed by massive parallel sequencing (ChIP-seq) has helped to identify transcription factor function and epigenetic modifications on a genome-wide scale. Most of these studies have classically been performed using cell line material. In this application note, we illustrate that the Bioruptor^® Pico (Diagenode) can process various human tumor tissue for ChIP-seq analyses of transcription factors as well as epigenetic marks.